| 1. |
|
|
| 2. |
|
|
| 3. |
|
|
| 4. |
- Callaway, EM, et al.
(författare)
-
A multimodal cell census and atlas of the mammalian primary motor cortex
- 2021
-
Ingår i: Nature. - : Springer Science and Business Media LLC. - 1476-4687 .- 0028-0836. ; 598:7879, s. 86-102
-
Tidskriftsartikel (refereegranskat)abstract
- Here we report the generation of a multimodal cell census and atlas of the mammalian primary motor cortex as the initial product of the BRAIN Initiative Cell Census Network (BICCN). This was achieved by coordinated large-scale analyses of single-cell transcriptomes, chromatin accessibility, DNA methylomes, spatially resolved single-cell transcriptomes, morphological and electrophysiological properties and cellular resolution input–output mapping, integrated through cross-modal computational analysis. Our results advance the collective knowledge and understanding of brain cell-type organization1–5. First, our study reveals a unified molecular genetic landscape of cortical cell types that integrates their transcriptome, open chromatin and DNA methylation maps. Second, cross-species analysis achieves a consensus taxonomy of transcriptomic types and their hierarchical organization that is conserved from mouse to marmoset and human. Third, in situ single-cell transcriptomics provides a spatially resolved cell-type atlas of the motor cortex. Fourth, cross-modal analysis provides compelling evidence for the transcriptomic, epigenomic and gene regulatory basis of neuronal phenotypes such as their physiological and anatomical properties, demonstrating the biological validity and genomic underpinning of neuron types. We further present an extensive genetic toolset for targeting glutamatergic neuron types towards linking their molecular and developmental identity to their circuit function. Together, our results establish a unifying and mechanistic framework of neuronal cell-type organization that integrates multi-layered molecular genetic and spatial information with multi-faceted phenotypic properties.
|
|
| 5. |
|
|
| 6. |
|
|
| 7. |
|
|
| 8. |
|
|
| 9. |
|
|
| 10. |
|
|
| 11. |
|
|
| 12. |
|
|
| 13. |
|
|
| 14. |
- Wang, K, et al.
(författare)
-
Long-term anti-inflammatory diet in relation to improved breast cancer prognosis: a prospective cohort study
- 2020
-
Ingår i: NPJ breast cancer. - : Springer Science and Business Media LLC. - 2374-4677. ; 6:1, s. 36-
-
Tidskriftsartikel (refereegranskat)abstract
- Inflammation-modulating nutrients and inflammatory markers are established cancer risk factors, however, evidence regarding the association between post-diagnosis diet-associated inflammation and breast cancer survival is relatively sparse. We aimed to examine the association between post-diagnosis dietary inflammatory index (DII®) and risks of all-cause and breast cancer-specific mortality. A total of 1064 female breast cancer survivors in the Prostate, Lung, Colorectal, and Ovarian Cancer Screening (PLCO) Trial prospective cohort, were included in this analysis if they had completed the diet history questionnaire (DHQ). Energy-adjusted DII (E-DIITM) scores were calculated based on food and supplement intake. Cox regression and competing risk models were used to estimate multivariable-adjusted hazards ratios (HRs) and 95% confidence intervals (95% CIs) by E-DII tertile (T) for all-cause and breast cancer-specific mortality. With median follow-up of 14.6 years, there were 296 (27.8%) deaths from all causes and 100 (9.4%) breast cancer-specific death. The E-DII was associated with all-cause mortality (HR T3 vs T1, 1.34; 95% CI, 1.01–1.81; Ptrend, 0.049, Table 2) and breast cancer mortality (HR T3 vs T1, 1.47; 95% CI, 0.89–2.43; Ptrend, 0.13; multivariable-adjusted HR for 1-unit increment: 1.10; 95% CI: 1.00–1.22). Non-linear positive dose–response associations with mortality from all causes were identified for E-DII scores (Pnon-linearity < 0.05). The post-diagnosis E-DII was statistically significantly associated with mortality risk among breast cancer survivors. Long-term anti-inflammatory diet might be a means of improving survival of breast cancer survivors.
|
|
| 15. |
|
|
| 16. |
|
|
| 17. |
- Zhang, JT, et al.
(författare)
-
Up-regulation of PINCH in the stroma of oral squamous cell carcinoma predicts nodal metastasis
- 2005
-
Ingår i: Oncology Reports. - 1021-335X .- 1791-2431. ; 14:6, s. 1519-1522
-
Tidskriftsartikel (refereegranskat)abstract
- Particularly interesting new cysteine-histidine rich protein (PINCH), an adapter protein involved in integrin and growth factor signalling, is up-regulated in the stroma of colorectal, breast, prostate, lung and skin cancer. Strong stromal immunostaining for PINCH is an independent prognostic indicator for reduced survival in colorectal cancer, suggesting that PINCH is involved in the signalling that promotes tumour progression. Since no study on PINCH has been carried out in oral squamous cell carcinoma (OSCC), this study aimed to determine PINCH expression in OSCC and its clinicopathological significance. PINCH protein expression was examined by immunohistochemistry in 20 normal oral mucosa and in 57 OSCC specimens, The frequency of strong PINCH immunostaining was higher in tumour-associated stroma of OSCC (37%) as compared to normal oral mucosa (10%) (p=0.02). Strong PINCH stromal immunostaining predicted nodal metastasis: 19/26 (73%) OSCC cases with nodal metastasis had strong PINCH immunostaining compared to 9/31 (29%) cases without nodal metastasis (p=0.02). The PINCH expression in OSCC was more intense in stroma at the invasive edge than in intratumoural stroma. In conclusion, the up-regulation of PINCH protein in stroma may be involved in promoting invasion and metastasis in OSCC.
|
|
| 18. |
- Zhang, QX, et al.
(författare)
-
Effects of limb elevation and increased intramuscular pressure on human tibialis anterior muscle blood flow
- 2001
-
Ingår i: European Journal of Applied Physiology. - : Springer Science and Business Media LLC. - 1439-6319 .- 1439-6327. ; 85:6, s. 567-571
-
Tidskriftsartikel (refereegranskat)abstract
- The effects of limb elevation and increased intramuscular pressure (IMP) on blood flow in the tibialis anterior muscle and leg neuromuscular function were studied in eight healthy subjects. Muscle blood flow (MBF) was measured by photoplethysmography using a custom-designed probe. IMP was elevated bilaterally by vein obstruction (60-65 mmHg) lasting 30 min induced by a thigh tourniquet of casted legs. Skin sensibility of the feet and the amplitude and area of the compound muscle action potentials from the extensor digitorum brevis muscle were evaluated. The subject kept one leg elevated 32 cm above heart level and the contralateral leg at heart level. All recordings were made before, during and after vein obstruction. IMP increased to 40 mmHg in the vein-obstructed casted legs. Perfusion pressure decreased from [mean (SD)] 42 (5.8) mmHg to 17 (6.4) mmHg in the elevated leg and from 65 (9.9) mmHg to 43 (8.4) mmHg in the non-elevated leg. MBF decreased by 50% in the elevated leg and by 42% in the non-elevated leg. Subjects experienced sensory dysfunction and muscular weakness in the elevated leg. In conclusion, increased IMP, induced by venous obstruction of a casted leg, reduced perfusion pressure and MBF, and resulted in a diminished amplitude and area of the compound muscle action potentials. Limb elevation above heart level combined with venous stasis of a casted leg further reduced perfusion pressure and MBF, and induced sensory dysfunction and muscular weakness.
|
|
| 19. |
|
|
