| 1. |
- Tian, Yupeng, et al.
(författare)
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Two novel two-photon polymerization initiators with extensive application prospects
- 2004
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Ingår i: Chemical Physics Letters. - : Elsevier. - 0009-2614 .- 1873-4448. ; 388:4-6, s. 325-329
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Tidskriftsartikel (refereegranskat)abstract
- Two novel two-photon polymerization initiators, 10-ethyl-3-E-(4-(N,N′-di-n-butylamino)styryl)phenothiazine 5 and 10-ethyl-3,7-E,E-bis(4-(N,N′-di-n-butyl amino)styryl)phenothiazine 6, have been efficiently synthesized with room temperature solid phase Wittig reaction. Two initiators were found to exhibit good single-photon fluorescence emission and their quantum yields, lifetimes and solvent effects have been studied in detail. Two-photon fluorescence spectra were investigated under 800 nm fs laser pulse and two-photon absorption (TPA) cross-sections of the initiators have been evaluated by theoretical calculation.Two-photon initiating polymerization microfabrication experiments have been carried out and artificial defects were made and polymerization mechanism was also discussed.
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| 2. |
- Zhang, Mingliang, et al.
(författare)
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Synthesis, crystal structure and two-photon property studies on a series of complexes derived from a novel Schiff base ligand
- 2004
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Ingår i: Transition metal chemistry (Weinheim). - : Kluwer Academic Publishers. - 0340-4285 .- 1572-901X. ; 29:6, s. 596-602
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Tidskriftsartikel (refereegranskat)abstract
- A new Schiff base ligand derived from S-benzyldithiocarbazate and 4-[N-hydroxy ethyl-N-(methyl)amino]benzaldehyde (HL, where H is a dissociable proton) and its NiII, CuII, ZnII and PdII complexes were prepared and fully characterized. The structures of HL and Ni(L)2 were determined by X-ray diffraction analysis, which revealed that the geometry of the NiII ion is square-planar with two equivalent Ni=N and Ni=S bonds, and that the two neighboring molecules in two layers have weak contact. The electronic spectra and solution fluorescence of the ligand and the complexes were studied, and the quantum yields of single-photon fluorescence for the compounds were determined. The compounds possess two-photon absorption (t.p.a.) character and the t.p.a. coefficient β and t.p.a. cross-section σ were determined by the Z-scan technique. Especially, the Zn(L)2complex and the HL ligand exhibit intensive two-photon fluorescence (t.p.f.) at 800 nm laser pulses in the femtosecond regime.
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| 3. |
- Zhang, Qiong, et al.
(författare)
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NIR-region two-photon fluorescent probes for Fe3+/Cu2+ ions based on pyrimidine derivatives with different flexible chain
- 2016
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Ingår i: Sensors and actuators. B, Chemical. - : ELSEVIER SCIENCE SA. - 0925-4005 .- 1873-3077. ; 222, s. 574-578
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Tidskriftsartikel (refereegranskat)abstract
- Two novel NIR-region two-photon fluorescent probes CCP and COP, show strong fluorescence quenching and good ratiometric responses toward Fe3+ and Cu2+, respectively; and their two-photon fluorescence are reversible by the subsequent addition of EDTA. CCP and COP are valuable candidates for two-photon imaging in the biological transparency window. (C) 2015 Elsevier B.V. All rights reserved.
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| 4. |
- Fei, Yiqiu, et al.
(författare)
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Role of prebiotics in enhancing the function of next-generation probiotics in gut microbiota
- 2023
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Ingår i: Critical reviews in food science and nutrition. - : TAYLOR & FRANCIS INC. - 1040-8398 .- 1549-7852. ; 63:8, s. 1037-1054
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Forskningsöversikt (refereegranskat)abstract
- With the development of high-throughput DNA sequencing and molecular analysis technologies, next-generation probiotics (NGPs) are increasingly gaining attention as live bacterial therapeutics for treatment of diseases. However, compared to traditional probiotics, NGPs are much more vulnerable to the harsh conditions in the human gastrointestinal tract, and their functional mechanisms in the gut are more complex. Prebiotics have been confirmed to play a critical role in improving the function and viability of traditional probiotics. Defined as substrates that are selectively utilized by host microorganisms conferring a health benefit, prebiotics are also important for NGPs. This review summarizes potential prebiotics for use with NGPs and clarifies their characteristics and functional mechanisms. Then we particularly focus on illustrating the protective effects of various prebiotics by enhancing the antioxidant capacity and their resistance to digestive fluids. We also elucidate the role of prebiotics in regulating anti-bacterial effects, intestinal barrier maintenance, and cross-feeding mechanisms of NPGs. With the expanding range of candidate NGPs and prebiotic substrates, more studies need to be conducted to comprehensively elucidate the interactions between prebiotics and NGPs outside and inside hosts, in order to boost their nutritional and healthcare applications.
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| 5. |
- Hao, Fuying, et al.
(författare)
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Design, crystal structures and enhanced frequency-upconverted lasing efficiencies of a new series of dyes from hybrid of inorganic polymers and organic chromophores
- 2009
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Ingår i: Journal of Materials Chemistry. - : RSC Publishing. - 0959-9428 .- 1364-5501. ; 19:48, s. 9163-9169
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Tidskriftsartikel (refereegranskat)abstract
- A new series of hybrid dyes, trans-4-(4′-N,N-dialkylaminostyryl)-N-methyl pyridinium [Cd(SCN)3−]n [alkyl = Me(1), Et(2), Pr(3) and n–Bu(4)] have been successfully synthesized from hybrids of inorganic polymers and organic chromophores. The structures of four new hybrid dyes were characterized by single-crystal X-ray diffraction first. The particular features of the hybrid dyes are (1) the inorganic anions form isolated polymeric one-dimensional zig-zag chains of [Cd(SCN)3−]n with an extended π-conjugation system within the polymeric –[M–S–C≡N]–M… chain and high polarizabilities; and (2) the organic cationic chromophores determine their optical properties. Experimental results revealed that the TPA cross-section values and overall energy conversion efficiencies of hybrid dyes with different alkyl pendant groups have obviously increased from 1 to 4, and in particular, have been substantially enhanced compared to their corresponding iodide analogues 1′–4′. Therefore, we speculate that such increases are attributable to arrangements of infinite anionic [Cd(SCN)3−]n chains and synergic effects with chromophore cations.
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| 6. |
- Yao, Mingfei, et al.
(författare)
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Improved functionality of Ligilactobacillus salivarius Li01 in alleviating colonic inflammation by layer-by-layer microencapsulation
- 2021
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Ingår i: npj Biofilms and Microbiomes. - : Nature Research. - 2055-5008. ; 7:1
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Tidskriftsartikel (refereegranskat)abstract
- The low viability during gastrointestinal transit and poor mucoadhesion considerably limits the effectiveness of Ligilactobacillus salivarius Li01 (Li01) in regulating gut microbiota and alleviating inflammatory bowel disease (IBD). In this study, a delivery system was designed through layer-by-layer (LbL) encapsulating a single Li01cell with chitosan and alginate. The layers were strengthened by cross-linking to form a firm and mucoadhesive shell (similar to 10 nm thickness) covering the bacterial cell. The LbL Li01 displayed improved viability under simulated gastrointestinal conditions and mucoadhesive function. Almost no cells could be detected among the free Li01 after 2 h incubation in digestive fluids, while for LbL Li01, the total reduction was around 3 log CFU/mL and the viable number of cells remained above 6 log CFU/mL. Besides, a 5-fold increase in the value of rupture length and a two-fold increase in the number of peaks were found in the (bacteria-mucin) adhesion curves of LbL Li01, compared to those of free Li01. Oral administration with LbL Li01 on colitis mice facilitated intestinal barrier recovery and restoration of the gut microbiota. The improved functionality of Li01 by LbL encapsulation could increase the potential for the probiotic to be used in clinical applications to treat IBD; this should be explored in future studies.
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| 7. |
- Fei, Yiqiu, et al.
(författare)
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The Role of Dihydroresveratrol in Enhancing the Synergistic Effect of Ligilactobacillus salivarius Li01 and Resveratrol in Ameliorating Colitis in Mice
- 2022
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Ingår i: Research. - : American Association for the Advancement of Science (AAAS). - 2096-5168 .- 2639-5274. ; 2022
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Tidskriftsartikel (refereegranskat)abstract
- Currently approved therapeutical strategies for inflammatory bowel diseases (IBD) suffer from variable efficacy and association with risk of serious side effects. Therefore, efforts have been made in searching for alternative therapeutics strategies utilizing gut microbiota manipulation. In this study, we show that the probiotic strain Ligilactobacillus salivarius Li01 (Li01) and the phytochemical prebiotic resveratrol (RSV) have synergistic effect in ameliorating colitis in mice. Oral coadministration of Li01 (10(9) CFU/d) and RSV (1.5 g/kg/d) promoted restoration of various inflammatory injuries and gut microbiota composition, exhibiting a favorable anti-inflammatory effect in DSS-induced colitis mice. The combination treatment was associated with reductions in the levels of proinflammatory cytokines IL-1 beta and IL-6 and increases in the levels of the anti-inflammatory cytokine IL-17A in mouse serum. Moreover, the combination treatment was found to alter the composition and metabolism of the gut microbiota, especially influencing the production of short chain fatty acids and anti-inflammatory related molecules. The mechanism underlying the improved anti-inflammatory effect from the RSV and Li01 combination treatment was found to be associated with the environmental sensor mammalian aryl hydrocarbon receptor (AHR) and tryptophan metabolism pathway. Administration of RSV in combination with Li01 in different mouse model led to enhanced conversion of RSV into metabolites, including dihydroresveratrol (DHR), resveratrol-sulfate, and resveratrol-glucuronide. DHR was found to be the dominant metabolite of RSV in conventional and colitis mice. An increased DHR/RSV ratio was confirmed to activate AHR and contribute to an enhanced anti-inflammatory effect. DHR is considered as a potential AHR ligand. The DHR/RSV ratio also affected the serotonin pathway by controlling the expression of Tph1, SERT, and 5-HT7R leading to amelioration of colitis in mice. Our data suggest that treatment with a combination of Li01 and RSV has potential as a therapeutic strategy for IBD; further investigation of this combination in clinical settings is warranted.
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| 8. |
- Lu, Shengyi, et al.
(författare)
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Effect of Experimental Periodontal Ligament Pain on Gingival Somatosensory Sensitivity
- 2017
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Ingår i: JOURNAL OF ORAL & FACIAL PAIN AND HEADACHE. - : Quintessence. - 2333-0384. ; 31:1, s. 72-79
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Tidskriftsartikel (refereegranskat)abstract
- Aims: To use a randomized, blinded, crossover design to evaluate the possible heterotopic effects of experimental periodontal ligament pain on adjacent gingival somatosensory sensitivity. Methods: A total of 12 healthy volunteers (8 female, 4 male; mean age standard error in means (SEM): 28 1 years) participated in two randomized experimental quantitative sensory testing (QST) sessions, one in which capsaicin (experimental) was injected into the periodontal ligament and one in which isotonic saline (control) was injected. A total of 13 standardized QST measures were obtained on the buccal attached gingiva of a maxillary central incisor before, immediately after, and 30 minutes after injection of 30 mu L of 5% capsaicin or isotonic saline into the periodontal ligament of the same incisor. The injection-evoked pain was evaluated on a 0-10 numeric rating scale (NRS). QST data were analyzed with two-way repeated measurement analysis of variance. Results: Capsaicin injected into the periodontal ligament evoked moderate levels of pain (mean peak NRS SEM: capsaicin: 5.5.7; control: 0.6 0.5 [P < .001]). Capsaicin injected into the periodontal ligament significantly modulated gingival somatosensory sensitivity: increased sensitivity to warmth and painful heat stimuli occurred immediately and 30 minutes after the injection (P < .025), whereas decreased sensitivity to both tactile and painful mechanical stimuli (P < .011) occurred immediately after the injection and to painful mechanical stimuli only after 30 minutes (P = .016). No somatosensory changes were detected following the injection of isotonic saline (P > .050). Conclusion: Capsaicin injected into the periodontal ligament caused gain of heterotopic somatosensory sensitivity toward warmth and painful heat stimuli as well as reduction in mechanical sensitivity of the gingiva adjacent to the injected tooth. These findings may have implications for interpretation of somatosensory functions in patients with chronic intraoral pain, where gingival somatosensory profiles similar to those detected after capsaicin injection in the present study may be interpreted as signs of nerve damage.
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| 9. |
- Lu, Shengyi, et al.
(författare)
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Somatosensory profiling of intra-oral capsaicin and menthol in healthy subjects
- 2013
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Ingår i: European Journal of Oral Sciences. - : John Wiley & Sons. - 0909-8836 .- 1600-0722. ; 121:1, s. 29-35
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Tidskriftsartikel (refereegranskat)abstract
- This study was designed to investigate the effect of surrogate orofacial pain models on the quantitative sensory testing (QST) profile in healthy participants. Capsaicin, menthol, or saline (control) were applied topically onto the gingiva of 15 healthy subjects for 15 min. During application, the subjects rated pain intensity on a score of 0-10, on an electronic visual analog scale (VAS). A standardized intra-oral QST protocol was performed before and immediately after application. Data obtained before and after application were compared using rank-sum tests, and QST profiles were made after Z-transformation. Application of capsaicin caused moderate levels of pain (VAS(peak) = 6.0 ± 0.7), and application of menthol produced mild levels of pain (VAS(peak) = 1.8 ± 0.6). Capsaicin induced hypersensitivity to warmth, heat pain and cold pain and hyposensitivity to mechanical stimuli. Menthol induced hypersensitivity to cold and warmth. Saline caused hypersensitivity to heat pain and hyposensitivity to mechanical stimuli. However, somatosensory profiles from Z-scores demonstrated sensory gains regarding warmth detection and heat pain only after application of capsaicin. In conclusion, a standardized battery of QST showed somatosensory changes after application of capsaicin, menthol and saline to the gingiva. However, the Z-score-based profiles may only reflect the most prominent somatosensory changes and thus represent a conservative approach for evaluation of data.
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| 10. |
- Lu, Yanmeng, et al.
(författare)
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The role of probiotic exopolysaccharides in adhesion to mucin in different gastrointestinal conditions
- 2022
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Ingår i: CURRENT RESEARCH IN FOOD SCIENCE. - : ELSEVIER. - 2665-9271. ; 5, s. 581-589
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Tidskriftsartikel (refereegranskat)abstract
- The presence of exopolysaccharides (EPS), a type of biomacromolecules, on the surface of probiotics play an important role in mucoadhesion, and it can be severely influenced by environments during gastrointestinal transit. In this study, the impact of gastrointestinal factors on surface properties of two probiotics (Lactobacillus rhamnosus GG and Pediococcus pentosaceus LI05) was investigated. Probiotic suspensions had relatively high viscosities and exhibited pronounced shear-thinning behavior due to the presence of EPS. The zeta-potential of both probiotics was relatively low and was not believed to play an important role in mucoadhesion. Compared to the control, the adhesive forces tended to decrease in the presence of gastric acids but increase in the presence of bile salts, since bile salts led to a thicker more open EPS layer compared to gastric acids. Although the functional groups of EPS in both probiotics are similar according to the study by FT-IR spectroscopy, the molecular weight of purified EPS in LI05 was much higher, ranging from 10,112 Da to 477,763 Da, which may contribute to higher rupture length in LI05 group. These results suggest that probiotic-mucin interactions are governed by the compositions and changes in the EPS of the probiotics in different gastrointestinal conditions, which contribute to a better understanding of the mucoadhesive behavior of the probiotics in the GIT.
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| 11. |
- Qiu, Bo, et al.
(författare)
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Enhanced gut microbiota delivery of a model probiotic (Faecalibacterium prausnitzii) : Layer-by-layer encapsulation using riboflavin-conjugated sodium alginate and glycol chitosan
- 2024
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Ingår i: Food Hydrocolloids. - : Elsevier. - 0268-005X .- 1873-7137. ; 154
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Tidskriftsartikel (refereegranskat)abstract
- Faecalibacterium prausnitzii (F. prausnitzii) exhibits a variety of biological functions that make it suitable for use as a next-generation probiotic. However, its high sensitivity to oxygen and digestive fluids currently limits its application. Riboflavin is known to support the growth of F. prausnitzii in oxygen environments, but it is important that it is in close proximity to the probiotics. Layer-by-layer assembly can be used to form protective coatings around probiotics, which can protect them from adverse environmental conditions. Moreover, riboflavin can be conjugated to these coatings, thereby increasing its efficacy by bringing it close to probiotic surfaces. In this study, we therefore evaluated the potential of electrostatic layer-by-layer assembly to protect F. prausnitzii by coating them with riboflavin-alginate and glycol-chitosan layers. Initially, we showed that riboflavin could be successfully conjugated to alginate, with a grafting ratio of around 4.35%. Then, the layer-by-layer method was used to coat F. prausnitzii using cationic glycol chitosan and anionic riboflavin-alginate. The coating formed was found to have a thickness of approximately 18.5 nm. Encapsulation did not adversely affect the growth of F. prausnitzii, but it significantly enhanced its resistance to oxygen and digestive fluids. The encapsulated probiotic was shown to have enhanced mucoadhesive properties using an in vitro intestinal monolayer model. Furthermore, the encapsulated probiotics colonized the colons of rats for longer than nonencapsulated ones. These results show that coating F. prausnitzii with riboflavin-rich biopolymer layers improves its resistance to oxygen and digestive fluids, and enhances its mucoadhesion and colonization properties, which should enhance its potential as an orally administered probiotic.
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| 12. |
- Qiu, Bo, et al.
(författare)
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Prevention of Loperamide-Induced Constipation in Mice and Alteration of 5-Hydroxytryotamine Signaling by Ligilactobacillus salivarius Li01
- 2022
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Ingår i: Nutrients. - : MDPI. - 2072-6643. ; 14:19
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Tidskriftsartikel (refereegranskat)abstract
- Although Ligilactobacillus salivarius Li01 (Li01) has shown much promise in preventing multiple gastrointestinal diseases, the potential of the probiotic in alleviating constipation and the related mechanisms remain unclear. In this study, the effects of Li01 were evaluated in a loperamide-induced constipation mouse model. The results demonstrated that Li01 intervention can relieve constipation symptoms by improving water content, quantity, and morphology of feces and act as an intestinal barrier structure protector. Furthermore, Li01 can modulate gut motility (gastrointestinal transit rate), the fluid transit-associated expression of aquaporins, and the serum parameters vasoactive intestinal peptide, substance P, and somatostatin. Constipation significantly increased the levels of 5-hydroxytryotamine (5-HT) in serum (p < 0.01) and decreased the levels in the intestine (p < 0.001). Due to its function of elevating the expression of tryptophan hydroxylase 1, this was reversed after Li01 treatment. Li01 also promoted the expression of 5-HT receptor 3 and 4, indicating that the 5-HT signaling pathway may play a critical role in the mechanism by which Li01 alleviate constipation symptoms. Additionally, Li01 significantly altered the gut microbiota composition by enhancing the ratio of Firmicutes/Bacteroidetes and increasing the abundance of Rikenellaceae_RC9 genera. Based on the above results, Li01 may have the potential to effectively alleviate constipation by regulating the 5-HT pathway and alteration of the gut microbiota.
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