| 2. |
- Klionsky, Daniel J., et al.
(författare)
-
Guidelines for the use and interpretation of assays for monitoring autophagy
- 2012
-
Ingår i: Autophagy. - : Informa UK Limited. - 1554-8635 .- 1554-8627. ; 8:4, s. 445-544
-
Forskningsöversikt (refereegranskat)abstract
- In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
|
|
| 3. |
- Li, Yiyin, et al.
(författare)
-
Pollen production estimates (PPEs) and fall speeds for major tree taxa and relevant source areas of pollen (RSAP) in Changbai Mountain, northeastern China
- 2015
-
Ingår i: Review of Palaeobotany and Palynology. - 0034-6667. ; 216, s. 92-100
-
Tidskriftsartikel (refereegranskat)abstract
- For model-based quantitative reconstructions of past vegetation cover on the scale of landscapes, pollen productivity estimates (PPEs) are key input parameters. In this study, we employed a random sampling strategy to collect moss polsters at 20 sites in Changbai Mountain, northeastern China. A detailed vegetation survey within 1000-m radius around each sampling point was carried out and digitized vegetation maps were used for, vegetation data compilation. A forest map at the scale of 1:25,000 was used to extract information about vegetation for the area between 1000 and 5000 m from each sampling point. Using the ERV (Extended R-Value) model, pollen productivity was estimated for Larix, Pinus, Juglans, Ulmus, Tilia, Betula and Fraxinus relative to Quercus. Estimates of pollen fall speeds for the eight taxa as well as the relevant source area of pollen (RSAP) were also obtained. Three different ERV sub-models were tested against the data. The sub-model 3 produced the best goodness of fit and the PPE values calculated with this sub-model show that BMA (5.04), Pinus (3.11), Juglans (1.94) and Ulmus (1.40) are high pollen producers with higher PPEs than Quercus while Fraxinus (0.76), Larix (0.30), Tilia (0.16) are low pollen producers compared to Quercus. The high pollen producers are all anemophilous species, while low pollen producing plants include both entomophilous, such as Fraxinus, Tilia and anemophilous species such as Larix. The estimated RSAP for the eight tree pollen taxa is about 2000-2500 m. (C) 2015 Elsevier B.V. All rights reserved.
|
|
