| 1. |
- Zhou, Yuye, et al.
(författare)
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Thiol-ene-based microfluidic chips for glycopeptide enrichment and online digestion of inflammation-related proteins osteopontin and immunoglobulin G
- 2023
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Ingår i: Analytical and Bioanalytical Chemistry. - : Springer Science and Business Media LLC. - 1618-2642 .- 1618-2650. ; 415:6, s. 1173-1185
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Tidskriftsartikel (refereegranskat)abstract
- Proteins, and more specifically glycoproteins, have been widely used as biomarkers, e.g., to monitor disease states. Bottom-up approaches based on mass spectrometry (MS) are techniques commonly utilized in glycoproteomics, involving protein digestion and glycopeptide enrichment. Here, a dual function polymeric thiol-ene-based microfluidic chip (TE microchip) was applied for the analysis of the proteins osteopontin (OPN) and immunoglobulin G (IgG), which have important roles in autoimmune diseases, in inflammatory diseases, and in coronavirus disease 2019 (COVID-19). TE microchips with larger internal surface features immobilized with trypsin were successfully utilized for OPN digestion, providing rapid and efficient digestion with a residence time of a few seconds. Furthermore, TE microchips surface-modified with ascorbic acid linker (TEA microchip) have been successfully utilized for IgG glycopeptide enrichment. To illustrate the use of the chips for more complex samples, they were applied to enrich IgG glycopeptides from human serum samples with antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The dual functional TE microchips could provide high throughput for online protein digestion and glycopeptide enrichment, showing great promise for future extended applications in proteomics and the study of related diseases.
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| 2. |
- Jia, Xiaomin, et al.
(författare)
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Single crystal metal-organic framework constructed by vertically self-pillared nanosheets and its derivative for oriented lithium plating
- 2021
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Ingår i: Cuihuà xuébào. - : Elsevier BV. - 0253-9837 .- 1872-2067. ; 42:9, s. 1553-1560
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Tidskriftsartikel (refereegranskat)abstract
- This vertically self-pillared (VSP) structure extends the application range of traditional porous materials with facile mass/ion transport and enhanced reaction kinetics. Here, we prepare a single crystal metal-organic framework (MOF), employing the ZIF-67 structure as a proof of concept, which is constructed by vertically self-pillared nanosheets (VSP-MOF). We further converted VSP-MOF into VSP-cobalt sulfide (VSP-CoS2) through a sulfidation process. Catalysis plays an important role in almost all battery technologies; for metallic batteries, lithium anodes exhibit a high theoretical specific capacity, low density, and low redox potential. However, during the half-cell reaction (Li++e=Li), uncontrolled dendritic Li penetrates the separator and solid electrolyte interphase layer. When employed as a composite scaffold for lithium metal deposition, there are many advantage to using this framework: 1) the VSP-CoS2 substrate provides a high specific surface area to dissipate the ion flux and mass transfer and acts as a pre-catalyst, 2) the catalytic Co center favors the charge transfer process and preferentially binds the Li+ with the enhanced electrical fields, and 3) the VSP structure guides the metallic propagation along the nanosheet 2D orientation without the protrusive dendrites. All these features enable the VSP structure in metallic batteries with encouraging performances.
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| 3. |
- Lu, Yahua, et al.
(författare)
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Engineer Nanoscale Defects into Selective Channels : MOF-Enhanced Li+ Separation by Porous Layered Double Hydroxide Membrane
- 2023
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Ingår i: Nano-Micro Letters. - 2311-6706. ; 15:1
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Tidskriftsartikel (refereegranskat)abstract
- Two-dimensional (2D) membrane-based ion separation technology has been increasingly explored to address the problem of lithium resource shortage, yet it remains a sound challenge to design 2D membranes of high selectivity and permeability for ion separation applications. Zeolitic imidazolate framework functionalized modified layered double hydroxide (ZIF-8@MLDH) composite membranes with high lithium-ion (Li+) permeability and excellent operational stability were obtained in this work by in situ depositing functional ZIF-8 nanoparticles into the nanopores acting as framework defects in MLDH membranes. The defect-rich framework amplified the permeability of Li+, and the site-selective growth of ZIF-8 in the framework defects bettered its selectivity. Specifically speaking, the ZIF-8@MLDH membranes featured a high permeation rate of Li+ up to 1.73 mol m−2 h−1 and a desirable selectivity of Li+/Mg2+ up to 31.9. Simulations supported that the simultaneously enhanced selectivity and permeability of Li+ are attributed to changes in the type of mass transfer channels and the difference in the dehydration capacity of hydrated metal cations when they pass through nanochannels of ZIF-8. This study will inspire the ongoing research of high-performance 2D membranes through the engineering of defects.
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| 4. |
- Wang, Y., et al.
(författare)
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Inherent mass transfer engineering of a Co, N co-doped carbon material towards oxygen reduction reaction
- 2021
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Ingår i: Journal of Energy Challenges and Mechanics. - : Elsevier BV. - 2056-9386. ; 58, s. 391-396
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Tidskriftsartikel (refereegranskat)abstract
- Current concerns on material-design induced mass transfer processes during small molecule electrocatalysis are on the ones assisted by external forced convection generally via electrode rotating, demonstrating the intrinsic activity of catalysts. Of note is that, in practical battery configurations, there is no the forced convection around electrode micro-environments. Therefore, the establishment of effective strategies in tuning the inherent mass transfer process, the one with no assistance by external forced convection, is also greatly significant, but rarely reported, retarding further advances. Herein, a size-induced inherent mass-transfer strategy is scrupulously established through designed kinetic investigations and also controllable construction of uniform Co, N co-doped carbon materials with a wide range of tunable particle sizes from 10 nm to 2 μm. The catalysts are synthesized by a pyrolysis of zeolitic imidazolate framework (ZIF) 67@ZIF-8, in which the wrapped shell layer avoids evident metal aggregations, and also contributes to rich porous environments after carbonizations. It is unclosed that particle size has a considerable effect on inherent mass transfer processes, even for the porous carbon catalysts. A particle size at around 700 nm is revealed to be most favorable for the inherent mass transfer process within the probed range, revealed by the smallest difference of Tafel slopes obtained with no electrode rotation and with infinite rotation speed. The latter is achieved via extrapolating rotation speeds to infinity in the Koutecký-Levich plots, by which the external mass transfer limitation can be completely eliminated. Contributed by the great inherent mass transfer process, the catalyst with a particle size of around 700 nm exhibits an impressive ORR activity in both three-electrode systems and zinc-air batteries. This work not only establishes a novel strategy in tuning inherent mass transfer process for small molecule electrocatalysis, more importantly, it provides a new dimension in kinetic investigations and oriented design of advanced energy materials.
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| 5. |
- Wang, Yanzhi, et al.
(författare)
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Two-Dimensional Metal-Organic Frameworks with Unique Oriented Layers for Oxygen Reduction Reaction : Tailoring the Activity through Exposed Crystal Facets
- 2022
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Ingår i: CCS CHEMISTRY. - : Chinese Chemical Society. - 2096-5745. ; 4:5, s. 1633-1642
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Tidskriftsartikel (refereegranskat)abstract
- As one of the most important families of porous materials, metal-organic frameworks (MOFs) have well-defined atomic structures. This provides ideal models for investigating and understanding the relationships between structures and catalytic activities at the molecular level. However, the active sites on the edges of two-dimensional (2D) MOFs have rarely been studied, as they are less exposed to the surfaces. Here, for the first time, we synthesized and observed that the 2D layers could align perpendicular to the surface of a 2D zeolitic imidazolate framework L (ZIF-L) with a leaf-like morphology. Owing to this unique orientation, the active sites on the edges of the 2D crystal structure could mostly be exposed to the surfaces. Interestingly, when another layer of ZIF-L-Co was grown heteroepitaxially onto ZIF-L-Zn (ZIF-L-Zn@ZIF-L-Co), the two layers shared a common b axis but rotated by 90 degrees in the ac plane. This demonstrated that we could control exposed facets of the 2D MOFs. The ZIF-L-Co with more exposed edge active sites exhibited high electrocatalytic activity for oxygen reduction reaction. This work provides a new concept of designing unique oriented layers in 2D MOFs to expose more edge-active sites for efficient electrocatalysis. [GRAPHICS] .
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| 6. |
- Xu, Xiaoxiang, et al.
(författare)
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Nanocarriers for Inner Ear Disease Therapy
- 2021
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Ingår i: Frontiers in Cellular Neuroscience. - : Frontiers Media SA. - 1662-5102. ; 15
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Forskningsöversikt (refereegranskat)abstract
- Hearing loss is a common disease due to sensory loss caused by the diseases in the inner ear. The development of delivery systems for inner ear disease therapy is important to achieve high efficiency and reduce side effects. Currently, traditional drug delivery systems exhibit the potential to be used for inner ear disease therapy, but there are still some drawbacks. As nanotechnology is developing these years, one of the solutions is to develop nanoparticle-based delivery systems for inner ear disease therapy. Various nanoparticles, such as soft material and inorganic-based nanoparticles, have been designed, tested, and showed controlled delivery of drugs, improved targeting property to specific cells, and reduced systemic side effects. In this review, we summarized recent progress in nanocarriers for inner ear disease therapy. This review provides useful information on developing promising nanocarriers for the efficient treatment of inner ear diseases and for further clinical applications for inner ear disease therapy.
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| 7. |
- Xu, Xiaoxiang, et al.
(författare)
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Zeolitic imidazolate framework-90 loaded with methylprednisolone sodium succinate effectively reduces hypertrophic scar in vivo
- 2024
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Ingår i: Nanoscale. - : Royal Society of Chemistry (RSC). - 2040-3364 .- 2040-3372. ; 16:13, s. 6708-6719
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Tidskriftsartikel (refereegranskat)abstract
- Hypertrophic scar (HS) is characterized by an abnormal fibroblast-myofibroblast transformation; non-apoptosis of fibroblasts; and redundant expression of TGF-beta 1, VEGF, alpha-SMA, and collagen I/III. An HS affects patients' physical and psychological quality of life, leading to joint dysfunction and skin cancer. However, there is currently no satisfactory drug to treat this disorder. In this study, we constructed methylprednisolone sodium succinate (MPSS) encapsulated ZIF-90 (MPSS@ZIF-90) for the effective treatment of an HS. The encapsulation of MPSS in ZIF-90 can achieve the controllable drug release of MPSS and prolong its effective treatment time. MPSS@ZIF-90 enhanced the apoptosis of human hypertrophic scar fibroblasts and downregulated the overexpression of TGF-beta 1, VEGF, alpha-SMA, and collagen I/III both in vitro and in vivo. The instant injection of MPSS@ZIF-90 effectively intervened with the formation of the HS after 28 days. On the contrary, MPSS@ZIF-90 greatly reduced the HS with two injections and 14 days of treatment after the HS was formed. This work provides evidence of effective intervention in the formation of an HS and the therapeutic effectiveness of MPSS@ZIF-90 with short treatment periods in vivo. It suggests that MPSS@ZIF-90 can be used as a biomedical option in the treatment of skin wounds and may reveal the potential molecular basis for promising future antifibrotic agents against scarring.
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| 8. |
- Zhou, Yuye, et al.
(författare)
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Amino Acid-Functionalized Two-Dimensional Hollow Cobalt Sulfide Nanoleaves for the Highly Selective Enrichment of N-Linked Glycopeptides
- 2020
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Ingår i: Analytical Chemistry. - : AMER CHEMICAL SOC. - 0003-2700 .- 1520-6882. ; 92:2, s. 2151-2158
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Tidskriftsartikel (refereegranskat)abstract
- Leaf-like hollow cobalt sulfides with a sulfur-gold-cysteine (S-Au-Cys) structure on the surface have been synthesized for efficient N-glycopeptide enrichment. A two-dimensional (2D) zeolitic imidazolate framework with cobalt (ZIF-L-Co, L for leaf) was used as a self-sacrificed template. After sulfidation, the S-Au-Cys architecture was created on the surface of the leaf-like hollow cobalt sulfide to obtain a material denoted ZIF-L-Co-S-Au-Cys. Enrichment of glycopeptides from trypsin digests of immunoglobulin G (IgG) standard samples and of IgG isolated from real human plasma samples was accomplished via hydrophilic interaction liquid chromatography processes using ZIF-L-Co-S-Au-Cys. The good sensitivity and selectivity ensure the effectiveness and robustness of ZIF-L-Co-S-Au-Cys for sample preconcentration, which is comparable to a commercial HILIC product. This work provides an efficient way to produce transition metal sulfides with a low-dimensional morphology and provides a novel concept for material design for exploitation in sample preparation, especially in glycoproteomics.
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| 9. |
- Zhou, Yuye, et al.
(författare)
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An antibody-free sample pretreatment method for osteopontin combined with MALDI-TOF MS/MS analysis
- 2019
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Ingår i: PLOS ONE. - : Public Library of Science. - 1932-6203. ; 14:3
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Tidskriftsartikel (refereegranskat)abstract
- Osteopontin is an osteoblast-secreted protein with an aspartic acid-rich, highly phosphorylated, and glycosylated structure. Osteopontin can easily bind to integrins, tumor cells, extracellular matrix and calcium, and is related to bone diseases, various cancers, inflammation etc. Here, DEAE-Cibacron blue 3GA was used to extract recombinant osteopontin from human plasma, and to deplete abundant plasma proteins with an antibody-free method. Using selected buffer systems, osteopontin and human serum albumin could be bound to DEAE-Cibacron blue 3GA, while immunoglobulin G was excluded. The bound osteopontin could then be separated from albumin by using different sequential elution buffers. By this method, 1 μg/mL recombinant osteopontin could be separated from the major part of the most abundant proteins in human plasma. After trypsin digestion, the extracted osteopontin could be successfully detected and identified by MALDI-TOF MS/MS using the m/z 1854.898 peptide and its fragments.
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| 10. |
- Zhou, Yuye
(författare)
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Analysis of proteins related toautoimmune and inflammatorydiseases with focus on newenrichment methodologies and MALDI-TOF MS
- 2020
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Proteins have been widely studied in biological sample analysis due to the association with diseases. In the present project, method and technique development was carried out on the analysis of two proteins, immunoglobulin G (IgG) and osteopontin (OPN). The changes in IgG glycosylation, and elevated plasma OPN levels have been reported to be associated with chronic inflammation and autoimmune diseases.Lab synthesized zeolitic imidazolate framework (ZIF) nanocomposites (paper I) and environmentally friendly wood materials (paper II) were successfully utilized for IgG glycopeptide enrichment in order to eliminate the interference caused by non‑glycopeptides. The quantification and identification of glycopeptides by matrix assisted laser desorption/ionization - time of flight mass spectrometry (MALDI-TOF MS) was simplified using label-free internal standard, and intact glycopeptide identification without glycan release. The separation of enriched glycopeptides was further studied on capillary electrophoresis.Due to the low abundance of OPN in plasma, preconcentration of OPN is required prior to MS analysis. In paper III, a fast, cheap and antibody-free method was developed for recombinant human OPN (rhOPN) preconcentration from a complex mixture, human plasma, together with MALDI-TOF/TOF MS identification.In proteomics, enzymatic digestion of proteins is a very common step. In paper IV, the utilization of thiol-ene microchips (TE microchip) immobilized with trypsin provided fast digestion with residence time of only 10 s. Furthermore, the TE microchip linked with ascorbic acid could be used for IgG glycopeptide enrichment. These applications made TE microchips ready for multifunctional tasks in proteomics.Methods and techniques developed in the present project can be applied in the future for the study of biological samples, to investigate the possible relation between these proteins and autoimmune and inflammatory diseases, as well as other related diseases.
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| 11. |
- Zhou, Yuye
(författare)
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Enrichment of glycopeptides using environmentally friendly wood materials
- 2020
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Ingår i: Green Chemistry. - 1463-9262 .- 1463-9270. ; 22, s. 5666-5676
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Tidskriftsartikel (refereegranskat)abstract
- Glycoproteomics is one of the main routes to study protein post-translational modifications, and enrichment is one of the important steps. Many hydrophilic materials have been used for glycopeptide enrichment, however involving complex synthesis procedures, high consumption in cost, time, and chemicals. Here, balsa wood, a naturally available hydrophilic material with highly porous structure, was investigated for immunoglobulin G (IgG) glycopeptide enrichment in a micropipette set-up. Native balsa wood without any pretreatment provided a green alternative to a commercial HILIC product, exhibiting comparable, or even better, enrichment performance for IgG standard and human plasma IgG samples. After delignification, binding capacity and recovery could be improved due to increased hydrophilicity and porosity. The developed method utilizing wood materials introduced an environmentally friendly option for glycoproteomics, saving both costs and chemicals, while exhibiting high performance.
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| 12. |
- Zhou, Yuye
(författare)
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Multifunctional thiol-ene microfluidic chips applied inprotein digestion and glycopeptide enrichment
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Annan publikation (övrigt vetenskapligt/konstnärligt)abstract
- Proteins, and more specifically glycoproteins, have been widely used as biomarkers. Bottom-up approaches based on mass spectrometry are techniques commonly utilized in glycoproteomics, involving protein digestion and glycopeptide enrichment. In order to reduce the sample volume, and to increase the time-to-result efficiency, microfluidic devices have been developed and utilized in proteomics. Here, a polymeric thiol-ene-based microfluidic chip (TE microchip) was applied for the analysis of the proteins osteopontin (OPN) and immunoglobulin G (IgG), which have important roles in autoimmune diseases and inflammatory diseases. After immobilizing trypsin on a high surface “monolith” with ascorbic acid linkage inside the channels, TE microchips could be successfully utilized for protein digestion. Similar digestion efficiencies were obtained as compared to a conventional trypsin digestion method, albeit with residence times of only a few seconds using the TE microchips. Furthermore, the TE chip linked with ascorbic acid could be directly utilized for IgG glycopeptide enrichment, via hydrophilic interaction between glycopeptides and the hydrophilic surface of the TE microchips. Using enrichment, almost all non-glycopeptides could be eliminated, and 25 glycopeptides with 12 glycoforms could be detected from 2 μg IgG sample.
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| 13. |
- Zhou, Yuye, et al.
(författare)
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Rapid Profiling of the Glycosylation Effects on the Binding of SARS-CoV-2 Spike Protein to Angiotensin-Converting Enzyme 2 Using MALDI-MS with High Mass Detection
- 2024
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Ingår i: Analytical Chemistry. - : American Chemical Society (ACS). - 0003-2700 .- 1520-6882. ; 96:5, s. 1898-1905
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Tidskriftsartikel (refereegranskat)abstract
- The spike protein receptor-binding domain (RBD) of SARS-CoV-2 binds directly to angiotensin-converting enzyme 2 (ACE2), mediating the host cell entry of SARS-CoV-2. Both spike protein and ACE2 are highly glycosylated, which can regulate the binding. Here, we utilized high-mass MALDI-MS with chemical cross-linking for profiling the glycosylation effects on the binding between RBD and ACE2. Overall, it was found that ACE2 glycosylation affects the binding more strongly than does RBD glycosylation. The binding affinity was improved after desialylation or partial deglycosylation (N690) of ACE2, while it decreased after degalactosylation. ACE2 can form dimers in solution, which bind more tightly to the RBD than the ACE2 monomers. The ACE2 dimerization and the binding of RBD to dimeric ACE2 can also be improved by the desialylation or deglycosylation of ACE2. Partial deglycosylation of ACE2 increased the dimerization of ACE2 and the binding affinity of RBD and ACE2 by more than a factor of 2, suggesting its high potential for neutralizing SARS-CoV-2. The method described in the work provided a simple way to analyze the protein-protein interaction without sample purification. It can be widely used for rapid profiling of glycosylation effects on protein-protein interaction for glycosylation-related diseases and the study of multiple interactions between protein and protein aggregates in a single system.
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