SwePub - sökning: L773:1742 5468

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1.	Innocenti, Nicolas, 1986-, et al. (författare) Lognormality and oscillations in the coverage of high-throughput transcriptomic data towards gene ends 2013 Ingår i: Journal of Statistical Mechanics. - : Institute of Physics (IOP). - 1742-5468 .- 1742-5468. ; 2013:10, s. P10013- Tidskriftsartikel (refereegranskat)abstract High-throughput transcriptomics experiments have reached the stage where the count of the number of reads alignable to a given position can be treated as an almost-continuous signal. This allows us to ask questions of biophysical/biotechnical nature, but which may still have biological implications. Here we show that when sequencing RNA fragments from one end, as is the case on most platforms, an oscillation in the read count is observed at the other end. We further show that these oscillations can be well described by Kolmogorov's 1941 broken stick model. We investigate how the model can be used to improve predictions of gene ends (3' transcript ends), but conclude that with present data the improvement is only marginal. The results highlight subtle effects in high-throughput transcriptomics experiments which do not have a biological origin, but which may still be used to obtain biological information.

Innocenti, Nicolas, 1986-, et al. (författare)
Lognormality and oscillations in the coverage of high-throughput transcriptomic data towards gene ends
2013
Ingår i: Journal of Statistical Mechanics. - : Institute of Physics (IOP). - 1742-5468 .- 1742-5468. ; 2013:10, s. P10013-
Tidskriftsartikel (refereegranskat)abstract
- High-throughput transcriptomics experiments have reached the stage where the count of the number of reads alignable to a given position can be treated as an almost-continuous signal. This allows us to ask questions of biophysical/biotechnical nature, but which may still have biological implications. Here we show that when sequencing RNA fragments from one end, as is the case on most platforms, an oscillation in the read count is observed at the other end. We further show that these oscillations can be well described by Kolmogorov's 1941 broken stick model. We investigate how the model can be used to improve predictions of gene ends (3' transcript ends), but conclude that with present data the improvement is only marginal. The results highlight subtle effects in high-throughput transcriptomics experiments which do not have a biological origin, but which may still be used to obtain biological information.

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