| 1. |
- An, Jiabin, et al.
(författare)
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Inactivation of the CYLD Deubiquitinase by HPV E6 Mediates Hypoxia-Induced NF-kappa B Activation
- 2008
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Ingår i: Cancer Cell. - : Elsevier BV. - 1878-3686 .- 1535-6108. ; 14:5, s. 394-407
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Tidskriftsartikel (refereegranskat)abstract
- The biochemical mechanisms that underlie hypoxia-induced NF-kappa B activity have remained largely undefined. Here, we find that prolonged hypoxia-induced NF-kappa B activation is restricted to cancer cell lines infected with high-risk human papillomavirus (HPV) serotypes. The HPV-encoded E6 protein is necessary and sufficient for prolonged hypoxia-induced NF-kappa B activation in these systems. The molecular target of E6 in the NF-kappa B pathway is the CYLD lysine 63 (K63) deubiquitinase, a negative regulator of the NF-kappa B pathway. Specifically, hypoxia stimulates E6-mediated ubiquitination and proteasomal degradation of CYLD. Given the established role of NF-kappa B in human carcinogenesis, these findings provide a potential molecular/viral link between hypoxia and the adverse clinical outcomes observed in HPV-associated malignancies.
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| 2. |
- Chenouard, Nicolas, et al.
(författare)
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Objective comparison of particle tracking methods
- 2014
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Ingår i: Nature Methods. - : Springer Science and Business Media LLC. - 1548-7091 .- 1548-7105. ; 11:3, s. 281-U247
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Tidskriftsartikel (refereegranskat)abstract
- Particle tracking is of key importance for quantitative analysis of intracellular dynamic processes from time-lapse microscopy image data. Because manually detecting and following large numbers of individual particles is not feasible, automated computational methods have been developed for these tasks by many groups. Aiming to perform an objective comparison of methods, we gathered the community and organized an open competition in which participating teams applied their own methods independently to a commonly defined data set including diverse scenarios. Performance was assessed using commonly defined measures. Although no single method performed best across all scenarios, the results revealed clear differences between the various approaches, leading to notable practical conclusions for users and developers.
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| 3. |
- Huss-Marp, J, et al.
(författare)
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Can Immunoglobulin E-measurement replace challenge tests in allergic rhinoconjunctivits to grass pollen?
- 2011
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Ingår i: CLINICAL AND EXPERIMENTAL ALLERGY. - 0954-7894. ; 41:8, s. 1116-1124
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Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND: There is minimal data available concerning the dose-response relationship between allergen exposure and clinical reactivity for outdoor aeroallergens, such as timothy grass pollen. Timothy pollen-specific IgE (sIgE) determinations might assist in predicting the clinical reactivity in patients with allergic rhinoconjunctivitis (ARC). METHODS: Allergen-sIgE antibody levels of timothy grass pollen were correlated with individual threshold doses eliciting allergic reactions in skin prick test (SPT), conjunctival (CPT) and nasal (NPT) provocation tests in patients suffering from pollen-induced rhinoconjunctivitis and healthy controls. RESULTS: One hundred and four patients with ARC (median age: 27 years; range: 18-64; females: 58%) and 36 controls (25 years (22-77); females: 70%) were included in the study. Ninety-six percent of the patients showed a positive reaction in the nasal and 57% showed a positive reaction in the conjunctival provocation. With regarding to titrated SPT, 98% of the patients showed a positive skin test reaction; correlating with the level of sIgE for timothy (P < 0.001). For both provocation protocols, there was no correlation between the provocation concentration at the reaction and the level of sIgE for timothy. The ratio of sIgE/total IgE correlated with the dilution level of SPT (P < 0.002) and CPT (P < 0.01), respectively. CONCLUSION AND CLINICAL RELEVANCE: A dose-response relationship between the levels of sIgE and clinical outcome of timothy allergen exposure could not be established. Although IgE-determination remains an important key element in allergy diagnosis, provocation tests are procedures of choice if the clinical relevance of an allergen has to be confirmed.
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| 4. |
- Imran, Ali Shariq, et al.
(författare)
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An Analysis of Social Collaboration and Networking Tools in eLearning
- 2016
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Ingår i: Learning and Collaboration Technologies. LCT 2016. - Cham : Springer. - 9783319394824 ; , s. 332-343
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Konferensbidrag (refereegranskat)abstract
- Many online learning websites and learning management systems (LMS) provide social collaboration and networking tools to aid learning and to interact with peers for knowledge sharing. The benefit of collaborating with each other is certainly undeniable, such tools, however, can be a distraction from the actual tasks for learners. The paper presents a study on social media tools supported by various eLearning systems to understand the impact on students learning activities. A survey questionnaire is designed for this purpose. The data is collected from students who have had experience using different massive open online course (MOOC) eLearning platforms and LMS from various universities. The results of the survey indicate that more than 95Â % of the participants use at least one of the social tools in their daily life activities, and almost 84Â % of them have used these tools in connection with the eLearning systems. It is also interesting to note that 92Â % of the participants intend to use social tools for study purposes. The results indicate that there is a need to integrate more of these social media tools into eLearning systems.
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| 5. |
- Mulvaney, Eamon P., et al.
(författare)
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Expression of the TPa and TPβ isoforms of the thromboxane prostanoid receptor (TP) in prostate cancer : Clinical significance and diagnostic potential
- 2016
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Ingår i: Oncotarget. - : Impact Journals, LLC. - 1949-2553. ; 7:45, s. 73171-73187
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Tidskriftsartikel (refereegranskat)abstract
- The prostanoid thromboxane (TX)A2 plays a central role in haemostasis and is increasingly implicated in cancer progression. TXA2 signals through two T Prostanoid receptor (TP) isoforms termed TPa and TPβ, with both encoded by the TBXA2R gene. Despite exhibiting several functional and regulatory differences, the role of the individual TP isoforms in neoplastic diseases is largely unknown. This study evaluated expression of the TPa and TPβ isoforms in tumour microarrays of the benign prostate and different pathological (Gleason) grades of prostate cancer (PCa). Expression of TPβ was significantly increased in PCa relative to benign tissue and strongly correlated with increasing Gleason grade. Furthermore, higher TPβ expression was associated with increased risk of biochemical recurrence (BCR) and significantly shorter disease-free survival time in patients post-surgery. While TPa was more variably expressed than TPβ in PCa, increased/high TPa expression within the tumour also trended toward increased BCR and shorter diseasefree survival time. Comparative genomic CpG DNA methylation analysis revealed substantial differences in the extent of methylation of the promoter regions of the TBXA2R that specifically regulate expression of TPa and TPβ, respectively, both in benign prostate and in clinically-derived tissue representative of precursor lesions and progressive stages of PCa. Collectively, TPa and TPβ expression is differentially regulated both in the benign and tumourigenic prostate, and coincides with clinical pathology and altered CpG methylation of the TBXA2R gene. Analysis of TPβ, or a combination of TPa/TPβ, expression levels may have significant clinical potential as a diagnostic biomarker and predictor of PCa disease recurrence.
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| 6. |
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| 7. |
- Thordeman, Bengt
(författare)
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Skogstapetens datering
- 1948
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Ingår i: Fornvännen. - 0015-7813 .- 1404-9430. ; , s. 123-132
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)
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