1. |
- Meijers, Rob, et al.
(författare)
-
On the Enzymatic Activation of NADH
- 2001
-
Ingår i: Journal of Biological Chemistry. - 1083-351X. ; 276:12, s. 9316-9321
-
Tidskriftsartikel (refereegranskat)abstract
- Atomic (1 Å) resolution x-ray structures of horse liver alcohol dehydrogenase in complex with NADH revealed the formation of an adduct in the active site between a metal-bound water and NADH. Furthermore, a pronounced distortion of the pyridine ring of NADH was observed. A series of quantum chemical calculations on the water-nicotinamide adduct showed that the puckering of the pyridine ring in the crystal structures can only be reproduced when the water is considered a hydroxide ion. These observations provide fundamental insight into the enzymatic activation of NADH for hydride transfer.
|
|