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1.	Iranmanesh, Ida, et al. (författare) Acoustic micro-vortexing of fluids, beads and cells in disposible microfluidic chips 2015 Ingår i: MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences. - : Chemical and Biological Microsystems Society. - 9780979806483 ; , s. 1005-1007 Konferensbidrag (refereegranskat)abstract In this paper we demonstrate a multi-functional platform using ultrasound for vortexing of 20-μl volumes of different samples in polymer-based disposable chips. The method enables different vortexing functions such as mixing laminar flows, resuspension of a micro-pellet of magnetic beads as well as cell lysis for DNA extraction. The device consists of an inexpensive low-frequency, high power, horn-shaped langevin transducer which is typically used for cell disruption in larger volumes. By controlling the operating time of this device (from fractions of a second up to a minute) different functions can be achieved. In addition, to avoid the high-power-induced heating, a simple cooling system is used as a chip holder consisting of a PC fan-cooled aluminum heat sink. To demonstrate a sample preparation application, we perform on-chip cell lysis and DNA extraction.

Iranmanesh, Ida, et al. (författare)
Acoustic micro-vortexing of fluids, beads and cells in disposible microfluidic chips
2015
Ingår i: MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences. - : Chemical and Biological Microsystems Society. - 9780979806483 ; , s. 1005-1007
Konferensbidrag (refereegranskat)abstract
- In this paper we demonstrate a multi-functional platform using ultrasound for vortexing of 20-μl volumes of different samples in polymer-based disposable chips. The method enables different vortexing functions such as mixing laminar flows, resuspension of a micro-pellet of magnetic beads as well as cell lysis for DNA extraction. The device consists of an inexpensive low-frequency, high power, horn-shaped langevin transducer which is typically used for cell disruption in larger volumes. By controlling the operating time of this device (from fractions of a second up to a minute) different functions can be achieved. In addition, to avoid the high-power-induced heating, a simple cooling system is used as a chip holder consisting of a PC fan-cooled aluminum heat sink. To demonstrate a sample preparation application, we perform on-chip cell lysis and DNA extraction.

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