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- Olofsson, Karl, et al.
(author)
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High-content nucleus based 3D image cytometery of whole multicellular tumour spheroids
- 2018
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In: 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018. - : Chemical and Biological Microsystems Society. ; , s. 1433-1434
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Conference paper (peer-reviewed)abstract
- We have previously presented an ultrasonic standing wave (USW) based multicellular tumor spheroid (MCTS) culture platform where 100 MCST can be formed, processed and imaged while being retained in micro-wells [1]. This platform was used to show MCTS characterization based on on-chip microscopy and off-chip flow cytometry [2]. Here, we present a high-content 3D image cytometry method of whole MCTS based on DNA staining where spatial and functional information can be assessed.
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