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  • Sundström, Heléne, et al. (author)
  • Segregation to non-dividing cells in recombinant Escherichia coli fed-batch fermentation processes
  • 2004
  • In: Biotechnology Letters. - 0141-5492 .- 1573-6776. ; 26:19, s. 1533-1539
  • Journal article (peer-reviewed)abstract
    • In Escherichia coli fermentation processes, a drastic drop in viable cell count as measured by the number of colony forming units per ml (c.f.u. ml(-1)) is often observed. This phenomenon was investigated in a process for the production of the recombinant fusion protein, promegapoietin (PMP). After induction, the number of c.f.u. ml(-1) dropped to approximately 10% of its maximum though the biomass concentration continued to increase. Flow cytometric analysis of viability and intracellular concentration of PMP showed that almost all cells were alive and contributed to the production. Thus, the drop in the number of c.f.u. ml(-1) probably reflects a loss of cell division capability rather than cell death.
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Type of publication
journal article (1)
Type of content
peer-reviewed (1)
Author/Editor
Enfors, Sven-Olof (1)
Ledung, Erika (1)
Norrman, Bo (1)
Sundström, Heléne (1)
Wållberg, Fredrik (1)
Hewitt, Christopher ... (1)
University
Royal Institute of Technology (1)
Mälardalen University (1)
Language
English (1)
Research subject (UKÄ/SCB)
Natural sciences (1)
Engineering and Technology (1)
Year
2004 (1)

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