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- Tynngård, Nahreen, et al.
(author)
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Platelet adhesion changes during storage studied with a novel method using flow cytometry and protein-coated beads
- 2015
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In: Platelets. - : Taylor & Francis. - 0953-7104 .- 1369-1635. ; 26:2, s. 177-185
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Journal article (peer-reviewed)abstract
- The aim of the present study was to set up and evaluate a novel method for studies of platelet adhesion and activation in blood and platelet suspensions such as platelet concentrate (PC) samples using protein-coated polystyrene beads and flow cytometry. To demonstrate its usefulness, we studied PCs during storage. PCs were prepared by aphaeresis technique (n = 7). Metabolic variables and platelet function was measured on day 1, 5, 7 and 12 of storage. Spontaneous and TRAP-6-induced adhesion to fibrinogen-and collagen-coated beads was analyzed by flow cytometry. P-selectin and phosphatidyl serine (PS) expression was assessed on platelets bound to beads as well as on non-adherent platelets. Platelet adhesion to fibrinogen beads had increased by day 12 and adhesion to collagen beads at day 7 of storage (p<0.05). TRAP-6 stimulation significantly increased the platelet adhesion to fibrinogen beads (p<0.05) as well as the P-selectin and PS exposure on platelets bound to beads (p<0.01) during the first 7 days of storage, but by day 12, significant changes were no longer induced by TRAP-6 stimulation. We demonstrate that our adhesion assay using protein-coated polystyrene beads can be used to assess the adhesion properties of platelets during storage without the addition of red blood cells. Therefore it may offer a useful tool for future studies of platelet adhesive capacity in transfusion medicine and other settings.
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