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- He, Mingyue, et al.
(författare)
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Printing protein arrays from DNA arrays
- 2008
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Ingår i: Nature Methods. - 1548-7091 .- 1548-7105. ; 5:2, s. 175-177
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Tidskriftsartikel (refereegranskat)abstract
- We describe a method, DNA array to protein array (DAPA), which allows the 'printing' of replicate protein arrays directly from a DNA array template using cell-free protein synthesis. At least 20 copies of a protein array can be obtained from a single DNA array. DAPA eliminates the need for separate protein expression, purification and spotting, and also overcomes the problem of long-term functional storage of surface-bound proteins.
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