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  • Aguda, Adeleke Halilu, et al. (author)
  • Expression, crystallization and preliminary crystallographic data analysis of filamin A repeats 14-16
  • 2007
  • In: Acta Crystallographica. Section F. - 1744-3091 .- 1744-3091. ; 63:4, s. 291-293
  • Journal article (peer-reviewed)abstract
    • Human filamin A is a 280 kDa protein involved in actin-filament cross-linking. It is structurally divided into an actin-binding headpiece (ABD) and a rod domain containing 24 immunoglobulin-like (Ig) repeats. A fragment of human filamin A (Ig repeats 14-16) was cloned and expressed in Escherichia coli and the purified protein was crystallized in 1.6 M ammonium sulfate, 2% PEG 1000 and 100 mM HEPES pH 7.5. The crystals diffracted to 1.95 A and belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 50.63, b = 52.10, c = 98.46 A, alpha = beta = gamma = 90 degrees.
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Type of publication
journal article (1)
Type of content
peer-reviewed (1)
Author/Editor
Aguda, Adeleke Halil ... (1)
Sakwe, Amos Malle (1)
Rask, Lars (1)
Robinson, Robert C. (1)
University
Uppsala University (1)
Language
English (1)
Year

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