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  • Rising, A., et al. (author)
  • Major ampullate spidroins from Euprosthenops australis : multiplicity at protein, mRNA and gene levels
  • 2007
  • In: Insect molecular biology (Print). - : Wiley. - 0962-1075 .- 1365-2583. ; 16:5, s. 551-561
  • Journal article (peer-reviewed)abstract
    • Spider dragline silk possesses extraordinary mechanical properties. It consists of large fibrous proteins called spidroins that display modular structures. It is known to consist of two proteins: the major ampullate spidroin (MaSp) 1 and MaSp2. This study analyses MaSp sequences from the nursery-web spider Euprosthenops australis. We have identified a previously uncharacterized MaSp2 sequence and a new MaSp-like spidroin, which display distinct homogenous submotifs within their respective Gly-rich repeats. Furthermore, a group of MaSp1 cDNA clones show unexpected heterogeneity. Genomic PCR identified several MaSp1 gene variants within individual spiders, which suggests the presence of a gene cluster in E. australis. Finally, the evolution of spidroin genes is discussed in relation to phylogenetic analysis of nonrepetitive C-terminal domains from diverse species.
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Type of publication
journal article (1)
Type of content
peer-reviewed (1)
Author/Editor
Johansson, J (1)
Larson, Greger (1)
Bongcam Rudloff, Eri ... (1)
Rising, A (1)
Hjälm, G (1)
Engström, W (1)
University
Uppsala University (1)
Karolinska Institutet (1)
Language
English (1)
Research subject (UKÄ/SCB)
Natural sciences (1)
Year

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