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  • Nordengrahn, Ann, et al. (author)
  • Evaluation of a novel proximity ligation assay for the sensitive and rapid detection of foot-and-mouth disease virus
  • 2008
  • In: Veterinary Microbiology. - : Elsevier BV. - 0378-1135 .- 1873-2542. ; 127:3-4, s. 227-236
  • Journal article (peer-reviewed)abstract
    • A novel proximity ligation assay (PLA) using a pan-serotype reactive monoclonal antibody was developed and evaluated for the detection of foot-and-mouth disease virus (FMDV) in clinical samples collected from field cases of disease. The FMDV-specific PLA was found to be 100 times more sensitive for virus detection than the commonly used antigen capture-ELISA (AgELISA). As few as five TCID50 were detected in individual assays, which was comparable with the analytical sensitivity of real-time RT-PCR. Although this assay was capable of detecting diverse isolates from all seven FMDV serotypes, the diagnostic sensitivity of the PLA assay was lower than real-time RT-PCR mainly due to a failure to detect some SAT 1, SAT 2 and SAT 3 FMDV strains. In conclusion, this new PLA format has high analytical sensitivity for the detection of FMDV in clinical samples and may prove valuable as a rapid and simple tool for use in FMD diagnosis.
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Type of publication
journal article (1)
Type of content
peer-reviewed (1)
Author/Editor
Landegren, Ulf (1)
Merza, Malik (1)
Gustafsdottir, Sigru ... (1)
Nordengrahn, Ann (1)
Ebert, Katja (1)
Reid, Scott M. (1)
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King, Donald P. (1)
Ferris, Nigel P. (1)
Brocchi, Emiliana (1)
Grazioli, Santina (1)
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University
Uppsala University (1)
Language
English (1)
Research subject (UKÄ/SCB)
Agricultural Sciences (1)
Year
2008 (1)

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