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- Jarvius, Jonas, et al.
(författare)
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Digital Quantification using Amplified Single-Molecule Detection
- 2006
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Ingår i: Nature Methods. - 1548-7091 .- 1548-7105. ; 3:9, s. 725-727
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Tidskriftsartikel (refereegranskat)abstract
- We describe a scheme for biomolecule enumeration by converting nanometer-scale specific molecular recognition events mediated by rolling-circle amplification to fluorescent micrometer-sized DNA molecules amenable to discrete optical detection. Our amplified single-molecule detection (SMD) approach preserves the discrete nature of the molecular population, allowing multiplex detection and highly precise quantification of molecules over a dynamic range of seven orders of magnitude. We apply the method for sensitive detection and quantification of the bacterial pathogen Vibrio cholerae.
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