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  • Larsson, Thomas, 1947, et al. (author)
  • Use of an affinity proteomics approach for the identification of low-abundant bacterial adhesins as applied on the Lewis(b)-binding adhesin of Helicobacter pylori.
  • 2000
  • In: FEBS letters. - 0014-5793. ; 469:2-3, s. 155-8
  • Journal article (peer-reviewed)abstract
    • Microbial attachment to host cell surfaces is considered to be the first essential step for colonization and infection. In most known cases, attachment is mediated by a specific protein-carbohydrate interaction. We have used a carbohydrate-containing crosslinking probe to select bacterial surface adhesins for trypsin digestion, MALDI-TOF mass spectrometry and identification against genome sequence. The present paper describes this functional proteomics approach for identification of the recently cloned low-abundant Lewis(b)-binding adhesin of Helicobacter pylori. Protein identification was obtained through the enrichment of approximately 300 fmol of adhesin from solubilized cells.
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Type of publication
journal article (1)
Type of content
peer-reviewed (1)
Author/Editor
Larsson, Thomas, 194 ... (1)
Bergström, Jörgen, 1 ... (1)
Karlsson, Karl-Ander ... (1)
Nilsson, Carol L, 19 ... (1)
University
University of Gothenburg (1)
Language
English (1)
Research subject (UKÄ/SCB)
Medical and Health Sciences (1)
Year

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