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- Vilanculos, Serafina, 1961, et al.
(author)
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Degradation of phytate in composite wheat/cassava/sorghum bread by activation of intrinsic cereal phytase
- 2021
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In: African Journal of Food Science. - 1996-0794. ; 15:1, s. 1-9
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Journal article (peer-reviewed)abstract
- The objective of this study was to degrade the phytic acid content in composite wheat/cassava/ sorghum bread by activating intrinsic cereal phytases during the baking process. The aim was to reach a phytate:iron molar ratio <1 to achieve an enhanced iron absorption in humans. Means to activate the phytase included dough preparation at different pH values and temperature as well as pre-soaking of the sorghum flour before baking. The phytic acid and mineral content was measured by high-performance ion chromatography. In the composite bread without pH adjustment of the dough, the phytate content was 1.58 μmol/g. After adjustment of the dough pH to 4.3, the phytate content in the composite bread decreased to 0.86 μmol/g. Soaking of the sorghum flour at 22°C for 3 h at pH 4.3 before baking, further decreased the phytate content to 0.58 μmol/g. Increasing the soaking temperature to 37°C and addition of 10% wheat flour resulted in a phytate content of 0.14 μmol/g in the composite bread, that is a reduction by 97% of the initial phytate content. The phytate:iron molar ratio was then 0.70 and the phytate:zinc molar ratio was 1.1 that is expected to have a positive effect on the absorption of both minerals in humans.
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