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1.	Dufva, Charlotte, et al. (författare) DNA profiles obtained from urine in snow 2019 Ingår i: Forensic Science International. - : ELSEVIER IRELAND LTD. - 1875-1768 .- 1875-175X. ; 7:1, s. 544-545 Tidskriftsartikel (refereegranskat)abstract Urine can be a potential important source of evidence when occurring at crime scenes. In case of outdoor scenes including snow a yellow colour could indicate the existence of human urine. In an effort to be able to investigate urine as a crime scene sample we have evaluated protocols for analysing DNA from urine in snow. Two different tests were performed with a smaller and a larger volume of urine dispensed on snow. The tubes were put into a freezer to mimic winter conditions. A Urine Preservative was added to some of the samples. DNA profiles were compared between samples extracted using a Urine DNA Isolation Kit and samples extracted with a Chelex-based method. In addition, a test was performed with the aim to mimic a potential crime scene. With a smaller volume of urine the best quality DNA profiles were obtained using the Urine DNA Isolation Kit without the Urine Preservative. When a larger volume of urine was handled and in the crime scene setup, there were no clear difference between the two extraction methods. Instead, the variation observed was between individuals.

Dufva, Charlotte, et al. (författare)
DNA profiles obtained from urine in snow
2019
Ingår i: Forensic Science International. - : ELSEVIER IRELAND LTD. - 1875-1768 .- 1875-175X. ; 7:1, s. 544-545
Tidskriftsartikel (refereegranskat)abstract
- Urine can be a potential important source of evidence when occurring at crime scenes. In case of outdoor scenes including snow a yellow colour could indicate the existence of human urine. In an effort to be able to investigate urine as a crime scene sample we have evaluated protocols for analysing DNA from urine in snow. Two different tests were performed with a smaller and a larger volume of urine dispensed on snow. The tubes were put into a freezer to mimic winter conditions. A Urine Preservative was added to some of the samples. DNA profiles were compared between samples extracted using a Urine DNA Isolation Kit and samples extracted with a Chelex-based method. In addition, a test was performed with the aim to mimic a potential crime scene. With a smaller volume of urine the best quality DNA profiles were obtained using the Urine DNA Isolation Kit without the Urine Preservative. When a larger volume of urine was handled and in the crime scene setup, there were no clear difference between the two extraction methods. Instead, the variation observed was between individuals.

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