SwePub
Sök i SwePub databas

  Extended search

Träfflista för sökning "onr:"swepub:oai:lup.lub.lu.se:d967b92c-0b29-43bd-a682-71dc908898d9" "

Search: onr:"swepub:oai:lup.lub.lu.se:d967b92c-0b29-43bd-a682-71dc908898d9"

  • Result 1-1 of 1
Sort/group result
   
EnumerationReferenceCoverFind
1.
  • Larrick, James W, et al. (author)
  • Polymemse chain reaction using mixed primers. Cloning of human monoclonal antibody variable region genes from single hybridoma cells
  • 1989
  • In: Bio/Technology. - : Springer Science and Business Media LLC. - 0733-222X. ; 7:9, s. 934-938
  • Journal article (peer-reviewed)abstract
    • We describe a general approach to rapidly obtain the DNA sequence encoding the variable region of any immunoglobulin chain using the polymerase chain reaction and a mixture of upstream primers corresponding to the leader sequence, and one downstream primer designed from the conserved nucleotide sequence of the constant region. The approach was applied to five different hybridomas producing human monoclonal antibodies and variable regions for both bold gamma and mu heavy chain and kappa and lambda light chain genes were successfully cloned. cDNA encoding variable regions could be amplified from single hybridoma cells isolated by micromanipulation. This approach will permit analysis of B cell clonal ontogeny, antibody diversity and lymphoma cell progression and heterogeneity. It will also facilitate structural and functional studies of immunoglobulins as well as the rapid construction of chimeric antibodies.
  •  
Skapa referenser, mejla, bekava och länka
  • Result 1-1 of 1
Type of publication
journal article (1)
Type of content
peer-reviewed (1)
Author/Editor
Borrebaeck, Carl (1)
Abrahamson, Magnus (1)
Danielsson, Lena (1)
Larrick, James W (1)
Brenner, Carol A (1)
Wallace, Ellen F (1)
show more...
Fry, Kirk E (1)
show less...
University
Lund University (1)
Language
English (1)
Research subject (UKÄ/SCB)
Medical and Health Sciences (1)
Year
1989 (1)

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Close

Copy and save the link in order to return to this view