| 11. |
|
|
| 12. |
- Enache, Andreea, et al.
(författare)
-
Demand for in-app purchases in mobile apps – A difference-in-difference approach Journal Pre-proof Demand for in-app purchases in mobile apps – A difference-in-difference approach
- 2023
-
Ingår i: International Journal of Industrial Organization. - : Elsevier B.V.. - 1873-7986 .- 0167-7187. ; 88
-
Tidskriftsartikel (refereegranskat)abstract
- Using five "freemium" mobile app games on six European markets we examine the effect of price changes on conversion rate, number of users and viewing of rewarded videos. Our difference-in-difference estimation relies on games being available on both the Apple and Google platforms with price changes on only one platform. Our main identification comes from exogenous adjustments of Apples prices in 2021. Own-price elasticities of conversion are in the -1 to -4 range. Watching of rewarded videos decreases as in-app prices decrease with an average elasticity of around.5, but overall play is not affected by changes in in-app prices.
|
|
| 13. |
- Forkel, Marianne, et al.
(författare)
-
Composition and functionality of the intrahepatic innate lymphoid cell-compartment in human nonfibrotic and fibrotic livers
- 2017
-
Ingår i: European Journal of Immunology. - : Wiley. - 0014-2980 .- 1521-4141. ; 47:8, s. 1280-1294
-
Tidskriftsartikel (refereegranskat)abstract
- Human innate lymphoid cells have been described to exist in different organs, with functional deregulation of these cells contributing to several disease states. Here, we performed the first detailed characterization of the phenotype, tissue-residency properties, and functionality of ILC1s, ILC2s, and ILC3s in the human adult and fetal liver. In addition, we investigated changes in the ILC compartment in liver fibrosis. A unique composition of tissue-resident ILCs was observed in nonfibrotic livers as compared with that in mucosal tissues, with NKp44− ILC3s accounting for the majority of total intrahepatic ILCs. The frequency of ILC2s, representing a small fraction of ILCs in nonfibrotic livers, increased in liver fibrosis and correlated directly with the severity of the disease. Notably, intrahepatic ILC2s secreted the profibrotic cytokine IL-13 when exposed to IL-33 and thymic stromal lymphopoetin (TSLP); these cytokines were produced by hepatocytes, hepatic stellate cells (HSCs), and Kupffer cells in response to TLR-3 stimulation. In summary, the present results provide the first detailed characterization of intrahepatic ILCs in human adult and fetal liver. The results indicate a role for ILC2s in human liver fibrosis, implying that targeting ILC2s might be a novel therapeutic strategy for its treatment.
|
|
| 14. |
|
|
| 15. |
- Friberg, Andrew S., et al.
(författare)
-
Human islet separation utilizing a closed automated purification system
- 2008
-
Ingår i: Cell Transplantation. - : SAGE Publications. - 0963-6897 .- 1555-3892. ; 17:12, s. 1305-1313
-
Tidskriftsartikel (refereegranskat)abstract
- A central step within the human islet isolation process is the separation of islets from contaminating exocrine tissue utilizing linear, continuous density gradients manufactured by means of manually controlled standard gradient makers (SGM). The present study was performed to develop a closed, automated purification system (APS) that customizes density gradient profiles aiming to standardize and optimize human islet purification. Digested human pancreata were pooled, split evenly, and incubated in UW solution according to our standard protocol (n = 11). Continuous density gradient centrifugation was performed in parallel in two refrigerated COBE 2991 cell separators loaded with light (1.076 g/ml) and heavy (1.097 g/ml) Ficoll utilizing either an SGM or two computer-controlled pumps connected to Ficoll-containing bags. Quality control included islet equivalent (IE) yield, purity, in vitro function, and islet cytokine expression. Gradient profiles demonstrated that the APS readily customizes linear and nonlinear gradients. In comparison to the SGM, the APS recovered a higher percentage of the expected volume of continuous gradients (90.0 +/- 1.1% vs. 98.2 +/- 2.0%, p < 0.05). Islet yield (120,468 +/- 15,970 vs. 114,570 +/- 15,313 IE, NS) and purity (51.7 +/- 4.8% vs. 54.4 +/- 4.9%, NS) were nearly identical utilizing the SGM or APS. Decreased MCP-1, IL-6, and IL-8 expression indicated that APS-purified islets were possibly exposed to less proinflammatory stress. Compared to standard procedures, similar success and gentle continuous density gradient separation of human islets is feasible utilizing the APS. The APS facilitates the standardization of this complex procedure according to cGMP standards.
|
|
| 16. |
- Friberg Hed, Niklas, et al.
(författare)
-
Arginase release in hemolytic uremic syndrome affects the vasculature
- 2023
-
Konferensbidrag (övrigt vetenskapligt/konstnärligt)abstract
- Hemolysis is a cardinal feature of hemolytic uremic syndrome (HUS) and during hemolysis excess arginase 1 is released from erythrocytes. Increased arginase activity leads to reduced arginine, as it is converted to urea and ornithine, and thereby reduced nitric oxide bioavailability, with secondary vascular injury. In this study we investigated arginase release in HUS patients and laboratory models. Two separate cohorts of patients (n=47 in total) with HUS associated with Shiga toxin-producing enterohemorrhagic E. coli (EHEC) and pediatric controls (n=35) were investigated. HUS patients had excessively high arginase 1 levels and activity (conversion to urea and ornithine) in plasma/serum during the acute phase, compared to remission and controls. Arginase 1 levels correlated with lactate dehydrogenase activity, indicating hemolysis, as well as the need for dialysis treatment. Patients also exhibited high levels of plasma alpha-1-microglobulin, a heme scavenger. Two mouse models were used, mice were challenged intragastrically with E. coli O157:H7 or injected intraperitoneally with Shiga toxin 2. Both models exhibited significantly elevated plasma arginase 1 levels and activity. Arginase 1 levels correlated with lactate dehydrogenase activity and alpha-1-microglobulin in the plasma of EHEC-infected mice. In vitro perfusion of Shiga toxin 2- and E. coli O157-lipopolysaccharide-stimulated human blood cells combined with ADAMTS13-deficient plasma over glomerular endothelial cells induced hemolysis and the release of bioactive arginase 1. The high levels of arginase released during HUS could thereby contribute to microvascular injury during HUS.
|
|
| 17. |
|
|
| 18. |
- Friberg, Magnus
(författare)
-
Tectonics of the middle Ural
- 2000
-
Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- This thesis concerns the crustal structure and tectonic evolution of the Middle Urals. Theresults are based on near-vertical reflection seismic profiling, geological fieldobservations and single zircon geochronology,The Ural Mountains delimit. the eastern margin of Baltica. Prior to PalaeozoicUralian orogeny, this margin was bounded by an ocean. The closing of this ocean wassimultaneous with the formation of a series of intra-oceanic island-arcs during theSilurian and Devonian. In the Middle Urals, these arcs collided with Baltica during theLate Carboniferous and Early Permian. New isotope-age data show that high-grademetaigneous complexes in the hinterland are not microcontinents, as previouslythought, but rather the roots of the island-arcs. The reflection seismic data reveal that thearcs are separated from the continent and each other by major thrusts, that are E-dippingin the west and W-dipping in the east. The suture between Baltica and the accretedterranes is the Main Uralian Fault Zone (MUFZ), a complex E-dipping deformationzone that records both early suturing and later extension. Normal displacements are alsoobserved on faults in the hinterland, some normal movement probably occurring at thesame time as the collision, and some related to Triassic opening of the West SiberianBasin (WSB). The deposits of the WSB now cover large parts of the eastern MiddleUrals. The seismic data show prominent, sub-horizontal reflections from the lower crust(38 to 43 km depth) under the WSB and the Middle Urals, east of the MUFZ. The Moho is, in most places, interpreted to be at the base of this reflective band. However, directly east of the MUFZ and for 50 km eastwards, the Moho, as identified from other geophysical methods, is at c. 50 km depth, i.e. below the base of the sub-horizontal reflectivity. The features observed in the lower crust, based on their truncation of the Palaeozoic structures and by comparison with seismic data from other areas, are interpreted to have formed from crustal stretching and to be related to the opening of the WSB.
|
|
| 19. |
- Friberg, Niklas, et al.
(författare)
-
Red blood cell-derived arginase release in hemolytic uremic syndrome
- 2024
-
Ingår i: Journal of Translational Medicine. - 1479-5876. ; 22:1
-
Tidskriftsartikel (refereegranskat)abstract
- BackgroundHemolysis is a cardinal feature of hemolytic uremic syndrome (HUS) and during hemolysis excess arginase 1 is released from red blood cells. Increased arginase activity leads to reduced L-arginine, as it is converted to urea and L-ornithine, and thereby reduced nitric oxide bioavailability, with secondary vascular injury. The objective of this study was to investigate arginase release in HUS patients and laboratory models and correlate arginase levels to hemolysis and kidney injury.MethodsTwo separate cohorts of patients (n = 47 in total) with HUS associated with Shiga toxin-producing enterohemorrhagic E. coli (EHEC) and pediatric controls (n = 35) were investigated. Two mouse models were used, in which mice were either challenged intragastrically with E. coli O157:H7 or injected intraperitoneally with Shiga toxin 2. An in vitro model of thrombotic microangiopathy was developed in which Shiga toxin 2- and E. coli O157 lipopolysaccharide-stimulated human blood cells combined with ADAMTS13-deficient plasma were perfused over glomerular endothelial cells. Two group statistical comparisons were performed using the Mann–Whitney test, multiple groups were compared using the Kruskal–Wallis test followed by Dunn’s procedure, the Wilcoxon signed rank test was used for paired data, or linear regression for continuous variables.ResultsHUS patients had excessively high plasma arginase 1 levels and activity (conversion of L-arginine to urea and L-ornithine) during the acute phase, compared to remission and controls. Arginase 1 levels correlated with lactate dehydrogenase activity, indicating hemolysis, as well as the need for dialysis treatment. Patients also exhibited high levels of plasma alpha-1-microglobulin, a heme scavenger. Both mouse models exhibited significantly elevated plasma arginase 1 levels and activity. Plasma arginase 1 levels correlated with lactate dehydrogenase activity, alpha-1-microglobulin and urea levels, the latter indicative of kidney dysfunction. In the in vitro model of thrombotic microangiopathy, bioactive arginase 1 was released and levels correlated to the degree of hemolysis.ConclusionsElevated red blood cell-derived arginase was demonstrated in HUS patients and in relevant in vivo and in vitro models. The excessively high arginase levels correlated to the degree of hemolysis and kidney dysfunction. Thus, arginase inhibition should be investigated in HUS.
|
|
| 20. |
- Friberg, Stina, et al.
(författare)
-
Grundutbildade sjuksköterskors upplevelse att börja arbeta på en barnavdelning
- 2016
-
Ingår i: Nordisk sygeplejeforskning. - : Scandinavian University Press / Universitetsforlaget AS. - 1892-2678 .- 1892-2686. ; 6:1, s. 20-33
-
Tidskriftsartikel (refereegranskat)abstract
- Basic trained nurses experience to start working on a children’s wardThe aim of the study was to describe nurses trained to a basic level experience of starting work on a children's ward. A qualitative study has been conducted with a purposively drawn sample of informants. Semi-structured interviews were conducted with eight basic trained nurses. Data were analyzed using qualitative content analysis. The results showed that respondents felt that the pediatrics course is not preparing for the professional work with children. Most felt that it was through real situations in the workplace that they gained necessary knowledge. Furthermore, it was found that only a few were satisfied with the induction. Spending time with many different supervisors was an important factor for experiencing dissatisfaction with the induction. Good support was received from employees; however the support of the organization was poor. Conclusion: Today's undergraduate education in nursing does not include enough pediatrics to prepare students for professional work with children and adolescents. Actions at individual and workplace levels are needed to increase knowledge of the basic trained nurse. Continuity and support during the induction is important for newly employed nurses to feel competent in their work.
|
|
