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| 13. |
- Byström, Jonas, et al.
(author)
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Eosinophil cationic protein is stored in, but not produced by, peripheral blood neutrophils
- 2002
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In: Clinical and Experimental Allergy. - : Wiley. - 0954-7894 .- 1365-2222. ; 32:7, s. 1082-1091
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Journal article (peer-reviewed)abstract
- BACKGROUND: Eosinophil cationic protein (ECP) is an eosinophil-derived protein, which has been shown to be present in circulating neutrophils. OBJECTIVE: To establish whether ECP is produced or internalized by peripheral blood neutrophils. METHODS: This was done using microscopy, flow cytometry, fractionation of cells and RT-PCR techniques. RESULTS: No ECP mRNA was detected after extensive cell purification to eliminate all traces of contaminating eosinophils. Examination of immunostained neutrophils by light, confocal, electron microscopy together with cell fraction experiments, established that ECP is present intracellularly and is mostly associated to cell granules. Uptake studies by flow cytometry and by using both cold and radiolabelled ECP showed that it is internalized by neutrophils and stored in some proportion in their primary granules. Upon stimulation with serum-treated Sephadex particles, the internalized ECP was partially released from cells. CONCLUSION: ECP is not produced but can be internalized by circulating neutrophils, which take it from the environment and partially store it in their primary granules.
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| 14. |
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| 15. |
- deWeert, Michael J., et al.
(author)
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Analysis of spatial variability in hyperspectral imagery of the uterine cervix in vivo
- 2003
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In: Proceedings of SPIE. - : SPIE. ; 4959, s. 67-76
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Journal article (peer-reviewed)abstract
- The use of fluorescence and reflectance spectroscopy in the analysis of cervical histopathology is a growing field of research. The majority of this research is performed with point-like probes. Typically, clinicians select probe sites visually, collecting a handful of spectral samples. An exception to this methodology is the Hyperspectral Diagnostic Imaging (HSDI®) instrument developed by Science and Technology International. This non-invasive device collects contiguous hyperspectral images across the entire cervical portio. The high spatial and spectral resolution of the HSDI instruments make them uniquely well suited for addressing the issues of coupled spatial and spectral variability of tissues in vivo. Analysis of HSDI data indicates that tissue spectra vary from point to point, even within histopathologically homogeneous regions. This spectral variability exhibits both random and patterned components, implying that point monitoring may be susceptible to significant sources of noise and clutter inherent in the tissue. We have analyzed HSDI images from clinical CIN (cervical intraepithelial neoplasia) patients to quantify the spatial variability of fluorescence and reflectance spectra. This analysis shows the spatial structure of images to be fractal in nature, in both intensity and spectrum. These fractal tissue textures will limit the performance of any point-monitoring technology.
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| 16. |
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| 17. |
- Håkansson, J., et al.
(author)
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Permability of felts
- 2000
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In: Proceedings of the 3rd Nordic Meeting on Materials and Mechanics. - Aalborg : Inst of mechanical engineering, Aalborg university. - 8789206312 ; , s. 351-359
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Conference paper (peer-reviewed)
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| 18. |
- Håkansson, J., et al.
(author)
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Permeability modelling of fibre networks
- 2002
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In: Annual transactions of the Nordic Rheology Society. - : Nordic Rheology Society.
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Conference paper (other academic/artistic)
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| 19. |
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| 20. |
- Håkansson, Mikael, 1957, et al.
(author)
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Chiral cis-octahedral Grignard reagents
- 2003
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In: Journal of Organometallic Chemistry. ; 681:10, s. 215-224
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Journal article (peer-reviewed)abstract
- Three chiral cis-octahedral Grignard reagents have been synthesized and structurally characterized by X-ray diffraction methods. Crystals of cis-[(n-Pr)MgBr(dme)2] (1), cis-[(i-Pr)MgBr(dme)2] (2) and cis-[(allyl)MgBr(dme)2] (3) were prepared from neat 1,2-dimethoxyethane (DME) and are all racemic. Synthesis and structural characterization of trans-[MgBr2(tmeda)2] (4) and cis-[MgBr2(dme)2] (5) indicated that bidentate tertiary amino ligands may be less well suited for the preparation of cis-octahedral Grignard reagents. On the other hand, the crystal structures of cis-[MgBr2(trigly)] (6) and [Mg2(μ-Br)2(trigly)2][Mg2(μ-Me)2Br4] (7) {trigly=triglyme)}, suggest that the triglyme ligand may be ideally suited for this purpose.
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