| 11. |
- Akaberi, Dario, et al.
(författare)
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Targeting the NS2B-NS3 protease of tick-borne encephalitis virus with pan-flaviviral protease inhibitors
- 2021
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Ingår i: Antiviral Research. - : Elsevier. - 0166-3542 .- 1872-9096. ; 190
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Tidskriftsartikel (refereegranskat)abstract
- Tick-borne encephalitis (TBE) is a severe neurological disorder caused by tick-borne encephalitis virus (TBEV), a member of the Flavivirus genus. Currently, two vaccines are available in Europe against TBEV. However, TBE cases have been rising in Sweden for the past twenty years, and thousands of cases are reported in Europe, emphasizing the need for antiviral treatments against this virus. The NS2B-NS3 protease is essential for flaviviral life cycle and has been studied as a target for the design of inhibitors against several well-known flaviviruses, but not TBEV. In the present study, Compound 86, a known tripeptidic inhibitor of dengue (DENV), West Nile (WNV) and Zika (ZIKV) proteases, was predicted to be active against TBEV protease using a combination of in silico techniques. Further, Compound 86 was found to inhibit recombinant TBEV protease with an IC50 = 0.92 mu M in the in vitro enzymatic assay. Additionally, two more peptidic analogues were synthetized and they displayed inhibitory activities against both TBEV and ZIKV proteases. In particular, Compound 104 inhibited ZIKV protease with an IC50 = 0.25 mu M. These compounds represent the first reported inhibitors of TBEV protease to date and provides valuable information for the further development of TBEV as well as pan-flavivirus protease inhibitors.
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| 12. |
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| 13. |
- Bengtsson, Anna, 1973-, et al.
(författare)
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The beneficial effect over 3 years by pictorial information to patients and their physician about subclinical atherosclerosis and cardiovascular risk : results from the VIPVIZA randomized clinical trial
- 2021
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Ingår i: American Journal of Preventive Cardiology. - : Elsevier. - 2666-6677. ; 7
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Tidskriftsartikel (refereegranskat)abstract
- Objective: Non-adherence to guidelines and preventive measures is a major challenge, particularly so to ob- tain long-term adherence to lifestyle changes and recommended medication. The objective was to investigate if pictorial information regarding subclinical carotid atherosclerosis provided to individuals and physicians gave sustained effects on cardiovascular risk beyond the previously reported effect after 1 year and up to 3 years. Methods: A Prospective Randomized Open Blinded End-point (PROBE) trial. Within a CVD prevention program in Västerbotten County, Sweden, 3532 healthy individuals aged 40, 50 or 60 years were enrolled and 1:1 ran- domized to intervention ( n = 1749; pictorial information with additional prevention materials to participants and physicians) or control group ( n = 1783; no pictorial information to participants and physicians). Preventive measures were managed within primary care. Participants were investigated at baseline during 2013–2016 and at follow-up after 1 and 3 years. Results: A beneficial effect on cardiovascular risk was observed at 3-year follow-up; Framingham Risk Score (FRS) was 13.38 for the intervention group and 14.08 for the control group ( p = 0.047) and SCORE was 1.69 vs. 1.82 ( p = 0.022). The effect observed at 1-year was sustained over 3 years after adjustment for sex and education and more pronounced among participants with a severe atherosclerotic picture at baseline.Conclusions: This study provides evidence of sustained beneficial effects on the adherence to prevention guidelines over 3 years of pictorial information about subclinical carotid atherosclerosis, resulting in lower cardiovascular risk regardless of sex and educational level. Direct visualization of the underlying still subclinical atherosclerotic disease, rather than just indirect information about risk factors and statistical risk of future myocardial infarction, stroke and death, is one way to tackle the problem of non-adherence to prevention of cardiovascular diseases.
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| 14. |
- Bergman, Anna Carin, et al.
(författare)
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dUTPase from the Retrovirus Equine Infectious Anemia Virus: High-Level Expression in Escherichia coli and Purification
- 1995
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Ingår i: Protein Expression and Purification. - : Elsevier BV. - 1046-5928. ; 6:3, s. 379-387
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Tidskriftsartikel (refereegranskat)abstract
- Deoxyuridine 5′-triphosphate nucleotidohydrolase (dUTPase, EC 3.6.1.23) catalyzes the hydrolysis of dUTP to dUMP and pyrophosphate, and plays important roles in nucleotide metabolism and DNA replication. The dUTPase gene of the retrovirus equine infectious anemia virus (EIAV) was cloned and overexpressed in Escherichia coli using the T7 RNA polymerase expression system. The recombinant vector (pET-3a/EDU), constructed by mutagenic PCR, was transformed into E. coli BL21(DE3) pLysS cells, resulting in expression of EIAV dUTPase at about 40% of the extracted protein, This level of overproduction is very high compared to previous reports on heterologous expression of dUTPases in E. coli. A one-step purification procedure using phosphocellulose chromatography results in a homogeneous preparation of the enzyme in a yield of 45 mg liter−1 of bacterial culture. The purified EIAV dUTPase, run on a sodium dodecyl sulfate-polyacrylamide gel electrophoresis, shows an apparent molecular mass of 15.1 kDa in accordance with the gene structure. The isoelectric point (pI) was determined to 5.6. Gel filtration under nondenaturating conditions gives a retention volume corresponding to a molecular mass of 40.8 kDa, suggesting a trimeric organization of the enzyme. The amino acid composition and amino-terminal sequence of the recombinant dUTPase are in agreement with predictions from the DNA sequence.
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| 15. |
- Bern, Sofia, et al.
(författare)
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Mobilitet och tillgänglighet – framtidens resande
- 2016
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- Trafikverket har gett TRUM (Transportforskningsenheten vid Umeå Universitet) i uppdrag att utreda framtidens mobilitet innefattande frågor såsom privatpersoners attityder och normer till resande med bil och däribland självkörande bilar, betalningsvilja, syn på ägande inställning till individuellt resande gentemot kollektivt resande, samt intressenters förväntningar på ett framtida tillgängligt transportsystem. Därutöver önskar Trafikverket ökad förståelse kring arbetssätt och metoder för att möta teknikutveckling och framtida mobilitet. Syftet med föreliggande rapport är att svara på dessa frågeställningar utifrån litteraturstudier och intervjuer.Det huvudsakliga underlaget för rapporten är en genomgång av litteratur relaterad till transporter, mobilitet och historiska såväl som framtida trender. Fokus har varit på persontransporter kopplat till människors beteende, samt vad privatpersoner, framtidsspanare och företrädare för bil- och teknikbranschen tror kommer att hända med personresande i framtiden. Utöver litteraturstudien genomfördes sex intervjuer under januari och februari 2016. Intervjuerna gjordes med syfte att öka förståelsen för hur människor resonerar kring mobilitet och transporter i dagsläget och hur de tror att vi kommer att resa i framtiden. Utifrån litteraturgenomgången och intervjuerna kan konstateras att attityder till elbilar generellt sett är positiva. Hinder för ett bredare genombrott utgörs av uppfattade prisskillnader, räckviddsångest och uppfattad brist på laddinfrastruktur. Som drivkrafter kan nämnas ökat miljömedvetande och alltmer positiva attityder bland flera olika aktörer, till exempel teknikutvecklare. När det gäller självkörande bilar är attityderna mer varierande. Bland annat lyfts säkerhetsfaktorer, tillgänglighet och det faktum att flera bilmodeller redan idag utrustas med olika typer av autonomifunktioner. Enligt olika bedömningar kommer koncept såsom Mobility as a Service och liknande att leda till förändrade attityder och normer när det gäller både privat och kollektivt resande. Avslutningsvis lämnas förslag på hur dessa och andra förändringar kan följas och förstås utifrån ett planeringsperspektiv. Olika metoder och perspektiv är viktiga för att skapa en bild över framtida utveckling redan idag, framförallt kopplat till de miljöutmaningar samhället står inför.
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| 16. |
- Berner, Logan T., et al.
(författare)
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The Arctic plant aboveground biomass synthesis dataset
- 2024
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Ingår i: Scientific Data. - : Springer Nature. - 2052-4463. ; 11:1
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Tidskriftsartikel (refereegranskat)abstract
- Plant biomass is a fundamental ecosystem attribute that is sensitive to rapid climatic changes occurring in the Arctic. Nevertheless, measuring plant biomass in the Arctic is logistically challenging and resource intensive. Lack of accessible field data hinders efforts to understand the amount, composition, distribution, and changes in plant biomass in these northern ecosystems. Here, we present The Arctic plant aboveground biomass synthesis dataset, which includes field measurements of lichen, bryophyte, herb, shrub, and/or tree aboveground biomass (g m−2) on 2,327 sample plots from 636 field sites in seven countries. We created the synthesis dataset by assembling and harmonizing 32 individual datasets. Aboveground biomass was primarily quantified by harvesting sample plots during mid- to late-summer, though tree and often tall shrub biomass were quantified using surveys and allometric models. Each biomass measurement is associated with metadata including sample date, location, method, data source, and other information. This unique dataset can be leveraged to monitor, map, and model plant biomass across the rapidly warming Arctic.
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| 17. |
- Byström, Johan, et al.
(författare)
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Using generalized Fibonacci sequences for solving the one-dimensional LQR problem and its discrete-time Riccati equation
- 2010
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Ingår i: Modeling, Identification and Control. - : Norwegian Society of Automatic Control. - 0332-7353 .- 1890-1328. ; 31:1, s. 1-18
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Tidskriftsartikel (refereegranskat)abstract
- In this article we develop a method of solving general one-dimensional Linear Quadratic Regulator (LQR) problems in optimal control theory, using a generalized form of Fibonacci numbers. We find the solution R (k) of the corresponding discrete-time Riccati equation in terms of ratios of generalized Fibonacci numbers. An explicit Binet type formula for R (k) is also found, removing the need for recursively finding the solution at a given timestep. Moreover, we show that it is also possible to express the feedback gain, the penalty functional and the controller state in terms of these ratios. A generalized golden ratio appears in the corresponding in finite horizon problem. Finally, we show the use of the method in a few examples.
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| 18. |
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| 19. |
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| 20. |
- Diaz de Grenu, Borja, et al.
(författare)
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Fluorescent Discrimination between Traces of Chemical Warfare Agents and Their Mimics
- 2014
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Ingår i: Journal of the American Chemical Society. - : American Chemical Society (ACS). - 0002-7863 .- 1520-5126. ; 136:11, s. 4125-4128
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Tidskriftsartikel (refereegranskat)abstract
- An array of fluorogenic probes is able to discriminate between nerve agents, sarin, soman, tabun, VX and their mimics, in water or organic solvent, by qualitative fluorescence patterns and quantitative multivariate analysis, thus making the system suitable for the in-the-field detection of traces of chemical warfare agents as well as to differentiate between the real nerve agents and other related compounds.
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