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| 17. |
- Friberg, M, et al.
(författare)
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Europrobe seismic reflection profiling across the eastern Middle Urals and West Siberian Basin
- 2001
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Ingår i: Terra Nova. - : Blackwell Publishing. - 0954-4879 .- 1365-3121. ; 12:6, s. 252-257
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Tidskriftsartikel (refereegranskat)abstract
- New deep seismic reflection data provide images of the crust and uppermost mantle underlying the eastern Middle Urals and adjacent West Siberian Basin. Distinct truncations of reflections delineate the late-orogenic strike-slip Sisert Fault extending vertically to ∼28 km depth, and two gently E-dipping reflection zones, traceable to 15–18 km depth, probably represent normal faults associated with the opening of the West Siberian Basin. A possible remnant Palaeozoic subduction zone in the lower crust under the West Siberian Basin is visible as a gently SW-dipping zone of pronounced reflectivity truncated by the Moho. Continuity of shallow to intermediate-depth reflections suggest that Palaeozoic accreted island-arc terranes and overlying molasse sequences exposed in the hinterland of the Urals form the basement for Triassic and younger deposits in the West Siberian Basin. A highly reflective lower crust overlies a transparent mantle at about 43 km depth along the entire 100 km long seismic reflection section, suggesting that the lower crust and Moho below the eastern Middle Urals and West Siberian Basin have the same origin.
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| 18. |
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| 19. |
- Johar, Dina, et al.
(författare)
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Inflammatory response, reactive oxygen species, programmed (necrotic-like and apoptotic) cell death and cancer
- 2004
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Ingår i: Roczniki Akademii Medycznej w Bialymstoku (1995). ; 49, s. 31-39
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Forskningsöversikt (refereegranskat)abstract
- In this short review we attempt to establish and/or strengthen connections between clinical, inflammatory manifestation of cancer, inflammatory processes driven by lipoxy-metabolites and their contribution to immortalized phenotype and apoptosis inhibition. Particularly the resemblance between symptoms of inflammation and signs associated with cancer chemotherapy and/or cytokine therapy is illustrated. In this context the role of apoptosis and necrosis in inflammation as well as the role of RedOx processes and lipid-oxidizing enzymes particularly cyclooxygenase-2 (COX-2) and also to lesser extend the 5-lipooxygenase (5-LOX) is highlighted. The multitude of biological effects of reactive oxygen species is shortly summarized and some aspects of it are being discussed in greater detail. Apoptotic cell death is discussed in the context of the "resolve-phase" of an inflammatory response. The disturbance of apoptosis is mainly deliberated in the framework of insufficient removal of immuno-effector cells that may cause autoimmunity. The role of COX-2 in apoptosis resistance is being highlighted mainly in the context of malignant transformation. The mechanism of cell death (apoptotic or necrotic) and its influence on the immune system and potential benefits of necrotic cell death induction during cancer chemotherapy is indicated.
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| 20. |
- Persson, R, et al.
(författare)
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dUTPase from Escherichia coli; high-level expression and one-step purification
- 2002
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Ingår i: Preparative Biochemistry & Biotechnology. - 1532-2297. ; 32:2, s. 157-172
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Tidskriftsartikel (refereegranskat)abstract
- The dut gene, which encodes Eseherichia coli deoxyuridine 5'-triphosphate nucleotidohydrolase (dUTPase), has been recloned to increase overexpression of the enzyme and to enable simplification of the purification protocol into a one-step procedure. The gene was cloned into the vector pET-3a and expressed in E. coli BL21(DE3) pLysS under the control of a bacteriophage T7 promotor. Induction results in production of dUTPase corresponding to 60% of the extracted protein. Phosphocellulose chromatography at low pH was utilised for one-step purification, resulting in a homogenous preparation of the recombinant protein with a highly specific activity. The yield of purified enzyme is 500mg per litre of bacterial culture, a significant increase compared to previously employed methods.
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