| 11. |
- Nath, Anjali K., et al.
(författare)
-
Proteomic-based detection of a protein cluster dysregulated during cardiovascular development identifies biomarkers of congenital heart defects
- 2009
-
Ingår i: PLOS ONE. - : PLOS. - 1932-6203. ; 4:1
-
Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND: Cardiovascular development is vital for embryonic survival and growth. Early gestation embryo loss or malformation has been linked to yolk sac vasculopathy and congenital heart defects (CHDs). However, the molecular pathways that underlie these structural defects in humans remain largely unknown hindering the development of molecular-based diagnostic tools and novel therapies.METHODOLOGY/PRINCIPAL FINDINGS: Murine embryos were exposed to high glucose, a condition known to induce cardiovascular defects in both animal models and humans. We further employed a mass spectrometry-based proteomics approach to identify proteins differentially expressed in embryos with defects from those with normal cardiovascular development. The proteins detected by mass spectrometry (WNT16, ST14, Pcsk1, Jumonji, Morca2a, TRPC5, and others) were validated by Western blotting and immunoflorescent staining of the yolk sac and heart. The proteins within the proteomic dataset clustered to adhesion/migration, differentiation, transport, and insulin signaling pathways. A functional role for several proteins (WNT16, ADAM15 and NOGO-A/B) was demonstrated in an ex vivo model of heart development. Additionally, a successful application of a cluster of protein biomarkers (WNT16, ST14 and Pcsk1) as a prenatal screen for CHDs was confirmed in a study of human amniotic fluid (AF) samples from women carrying normal fetuses and those with CHDs.CONCLUSIONS/SIGNIFICANCE: The novel finding that WNT16, ST14 and Pcsk1 protein levels increase in fetuses with CHDs suggests that these proteins may play a role in the etiology of human CHDs. The information gained through this bed-side to bench translational approach contributes to a more complete understanding of the protein pathways dysregulated during cardiovascular development and provides novel avenues for diagnostic and therapeutic interventions, beneficial to fetuses at risk for CHDs.
|
|
| 12. |
- Snyder, Kristen, 1964-, et al.
(författare)
-
Going hybrid on a dime : Lessons from schooling during the pandemic and implications for sustainable quality in education
- 2021
-
Ingår i: ICERI2021 Proceedings. - : IATED. - 9788409345496 ; , s. 4503-4512
-
Konferensbidrag (refereegranskat)abstract
- It is speculated that the Covid- 19 Pandemic has changed the future of work emphasizing hybrid solutions and networking. Understanding how distance education or hybrid models can be sustainable at the K-12 level is therefore of relevance to better prepare youth for a future world of work and sustainable development. The purpose of this paper is to present findings from phase two of the qualitative case study to examine more in-depth the ways in which a private school adapted it’s educational programming using technology-supported systems to innovate and maintain attractive quality education. Data were gathered from interviews, focus groups and video footage. The Digital Culture model was used as an analytical framework to examine the data from four dimension: pedagogy, communication, organizational systems, and technology.
|
|
| 13. |
- Snyder, Kristen, 1964-, et al.
(författare)
-
“Going hybrid on a dime” : Insights for transformation in education toward sustainable quality development
- 2023
-
Ingår i: Seminar.net. - : OsloMet - Oslo Metropolitan University. - 1504-4831. ; 19:1
-
Tidskriftsartikel (refereegranskat)abstract
- This study contributes to research on Quality in Education and examines what possibilities now exist for schools to reinvent and transform using technology-based systems as part of the equation. It is speculated that the pandemic has changed the future of work emphasizing hybrid solutions and networking. The purpose of this article is to present findings from phase two of the qualitative case study to examine what happened to a private school when it went “hybrid on a dime” to maintain attractive quality education.
|
|
| 14. |
|
|
| 15. |
|
|
| 16. |
|
|
| 17. |
|
|
| 18. |
|
|
| 19. |
|
|
| 20. |
|
|
