| 11. |
- Brakebusch, Cord, et al.
(författare)
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Brevican-deficient mice display impaired hippocampal CA1 long-term potentiation but show no obvious deficits in learning and memory
- 2002
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Ingår i: Molecular and Cellular Biology. - 0270-7306. ; 22:21, s. 7417-7427
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Tidskriftsartikel (refereegranskat)abstract
- Brevican is a brain-specific proteoglycan which is found in specialized extracellular matrix structures called perineuronal nets. Brevican increases the invasiveness of glioma cells in vivo and has been suggested to play a role in central nervous system fiber tract development. To study the role of brevican in the development and function of the brain, we generated mice lacking a functional brevican gene. These mice are viable and fertile and have a normal life span. Brain anatomy was normal, although alterations in the expression of neurocan were detected. Perineuronal nets formed but appeared to be less prominent in mutant than in wild-type mice. Brevican-deficient mice showed significant deficits in the maintenance of hippocampal long-term potentiation (LTP). However, no obvious impairment of excitatory and inhibitory synaptic transmission was found, suggesting a complex cause for the LTP defect. Detailed behavioral analysis revealed no statistically significant deficits in learning and memory. These data indicate that brevican is not crucial for brain development but has restricted structural and functional roles.
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| 12. |
- Carling, Tobias, et al.
(författare)
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Familial hypercalcemia and hypercalciuria caused by a novel mutation in the cytoplasmic tail of the calcium receptor
- 2000
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Ingår i: Journal of Clinical Endocrinology and Metabolism. - 0021-972X .- 1945-7197. ; 85:5, s. 2042-7
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Tidskriftsartikel (refereegranskat)abstract
- Familial hyperparathyroidism (HPT), characterized by hypercalcemia and hypercalciuria, and familial benign hypocalciuric hypercalcemia (FHH) are the most common causes of hereditary hypercalcemia. The calcium-sensing receptor (CaR) regulates PTH secretion and renal calcium excretion. Heterozygous inactivating mutations of the gene cause FHH, whereas CaR gene mutations have not been demonstrated in HPT. In a kindred with 20 affected individuals, the hypercalcemic disorder segregated with inappropriately higher serum PTH and magnesium levels and urinary calcium levels than in unaffected members. Subtotal parathyroidectomy revealed parathyroid gland hyperplasia/adenoma and corrected the biochemical signs of the disorder in seven of nine individuals. Linkage analysis mapped the condition to markers flanking the CaR gene on chromosome 3q. Sequence analysis revealed a mutation changing phenylalanine to leucine at codon 881 of the CaR gene, representing the first identified point mutation located within the cytoplasmic tail of the CaR. A construct of the mutant receptor (F881L) was expressed in human embryonic kidney cells (HEK 293), and demonstrated a right-shifted dose-response relationship between the extracellular and intracellular calcium concentrations. The hypercalcemic disorder of the present family is caused by an inactivating point mutation in the cytoplasmic tail of the CaR and displays clinical characteristics atypical of FHH and primary HPT.
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| 13. |
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| 16. |
- Halthur, Tobias, et al.
(författare)
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Stability of Polypeptide Multilayers As Studied by in Situ Ellipsometry : Effects of Drying and Post-Buildup Changes in Temperature and pH
- 2004
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Ingår i: Journal of the American Chemical Society. - : American Chemical Society (ACS). - 0002-7863 .- 1520-5126. ; 126, s. 17009-17015
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Tidskriftsartikel (refereegranskat)abstract
- Polyelectrolyte multilayers (PEM) of poly(L-glutamic acid) (PGA) and poly(L-lysine) (PLL) with an initial layer of polyethyleneimine (PEI) were built on silica and titanium surfaces using the layer-by-layer (LbL) technique. The stability of the film during drying/rewetting, temperature cycles, and pH shifts was studied in situ by means of ellipsometry. The film thickness was found to decrease significantly (approximately 70%) upon drying, but the original film thickness was regained upon rewetting, and the buildup could be continued. The thickness in the dry state was found to be extremely sensitive to ambient humidity, needing several hours to equilibrate. Changes in temperature and pH were also found to influence the multilayer thickness, leading to swelling and deswelling of as much as 8% and 10-20% respectively. The film does not necessarily regain its original thickness as the pH is shifted back, but instead shows clear signs of hysteresis.
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| 17. |
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| 18. |
- Larsson, Tobias Erik Martin, 1976-
(författare)
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The Role of Fibroblast Growth Factor 23 in Phosphate Homeostasis
- 2004
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- The regulation of serum phosphate (Pi) concentrations is a complex process and our current models are far from complete. Due to major advancements in biotechnology and the development of more powerful research tools, recent advances in the field of genetics has led to the identification of several candidates for the long sought-after phosphatonin(s), or Pi regulating hormones. One of these candidates is fibroblast growth factor 23 (FGF-23) and this thesis is based upon studies of the role of FGF-23 in Pi homeostasis. We demonstrate that FGF-23 is a secreted protein which is highly expressed in tumors giving rise to oncogenic hypophosphatemic osteomalacia (OOM). Furthermore, we have developed a two-site enzyme-linked immunosorbent assay for the detection of circulating FGF-23 and established that FGF-23 is present in the circulation of healthy individuals. Also, FGF-23 serum levels are elevated in patients with disturbances in Pi homeostasis such as OOM, X-linked hypophosphatemic rickets (XLH) and chronic kidney disease and are likely to play an important role in the pathogenesis of these disorders. A transgenic mouse model that express human FGF-23 under the control of the α1(I) collagen promoter exhibit similar clinical and biochemical characteristics as do patients with OOM, XLH and autosomal dominant hypophosphatemic rickets indicating that FGF-23 is an important determinant of Pi homeostasis, vitamin D metabolism and bone mineralization.
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| 19. |
- Larsson, Tord, et al.
(författare)
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Industrialiserat byggande : en granskning av några olika metoder
- 2004
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- Det finns idag (2004) ett flertal exempel på prefabbyggen med lätta stommar. Byggelementen är antingen volymelement eller planelement eller kombinationer som på byggplatsen monteras ihop tillsammans med den för husets funktion viktiga infrastrukturen såsom grund, trapphus, installationsstammar och apparatrum. Tre olika koncept har undersökts nämligen Move In®, Bo Klok och B4. Move In är ett volymelementkoncept med moduler som innefattar stjärnhus, punkthus, lamellhus och vinkelhus. Bo Klok är ett volymelementkoncept med moduler och byggs som vinkelhus medan B4 är planelementkoncept för olika typer av flerbostadshus. Det är möjligt att bygga upp till fyra våningar med Move In och B4, medan Bo Klok konceptet är ett två våningskoncept. Vid byggprojekt med byggelement med lätta stommar är det viktigt att så tidigt som möjligt starta processen att planera för prefabbygge. Denna start skall ske redan i programstadiet. Både projekterings- och produktionsskedet är annorlunda än vid traditionella platsbyggda projekt. Särskilt elementens storlekt vid transporten styr storleksmått på planlösningen i husen. Samordning mellan de delar som kommer att bli platsbyggda infrastrukturen är viktig. I rapporten redovisas synpunkter från arkitekter, byggtekniker, statiker och installationstekniker, från modulproduktionen och från förvaltare. Vidare redovisas för ett tiotal materialval och Move Ins motiv till varför de valt just detta material. För samma material ger sedan någon tillverkare och förvaltare sin syn på dessa material. Detta gäller för fönster, våtrumsskivor, träullskivor, klinker, linoleummatta, utomhus - och inomhusfärg, trästomme, grundläggning och köksluckor. Motivet till Move Ins val är att lösningarna som givet dessa materialval ofta är grundade på underhålls- och miljöaspekter. Rapporten är till viss del en sammanfattning och en analys av två examensarbeten, referens (Nygren, 2003)[15] och (Ellervik och Brorsson, 2004) [17].
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