341. |
- Palm-Apergi, C, et al.
(författare)
-
Protein Delivery by PTDs/CPPs
- 2022
-
Ingår i: Methods in molecular biology (Clifton, N.J.). - New York, NY : Springer US. - 1940-6029. ; 2383, s. 257-264
-
Tidskriftsartikel (refereegranskat)
|
|
342. |
|
|
343. |
- Pasetto, A, et al.
(författare)
-
T-Cell Repertoire Characterization
- 2022
-
Ingår i: Methods in molecular biology (Clifton, N.J.). - New York, NY : Springer US. - 1940-6029. ; 2574, s. 209-219
-
Tidskriftsartikel (refereegranskat)
|
|
344. |
|
|
345. |
- Pejler, Gunnar, et al.
(författare)
-
Serglycin- the master of the mast cell
- 2012
-
Ingår i: Methods in Molecular Biology. - Totowa, NJ : Humana Press. - 1064-3745 .- 1940-6029. ; 836, s. 201-217
-
Tidskriftsartikel (refereegranskat)
|
|
346. |
|
|
347. |
|
|
348. |
|
|
349. |
- Persson, Andrea, et al.
(författare)
-
Production and HPLC-Based Disaccharide Analysis of Xyloside-Primed Glycosaminoglycans
- 2022
-
Ingår i: Glycosaminoglycans. Methods in Molecular Biology, vol 2303. Balagurunathan K., Nakato H., Desai U., Saijoh Y. (eds). - New York, NY : Springer. - 1064-3745 .- 1940-6029. - 9781071613986 ; , s. 173-182
-
Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
- Although glycosaminoglycans (GAGs) are known to be involved in a variety of physiological and pathological processes, knowledge about their expression by cells or tissues, the GAGome, is limited. Xylosides can be used to induce the formation of GAGs without the presence of a proteoglycan core protein. The administration of xylosides to living cells tends to result in a considerable amplification in GAG production, and the xylosides can, therefore, be used as analytical tools to study the GAG produced by a certain cell type. One of the most common ways to analyze the GAGs structurally is by disaccharide analysis, which involves depolymerization of the GAGs into disaccharides, fluorescent labeling of the disaccharides with 2-aminoacridone, and quantification using high-pressure liquid chromatography (HPLC). Here, we describe the procedure of producing xyloside-primed GAGs and how to study them structurally by disaccharide analysis. © 2022, Springer Science+Business Media, LLC, part of Springer Nature.
|
|
350. |
- Persson, Helena, et al.
(författare)
-
Antibody validation by immunoprecipitation followed by mass spectrometry analysis
- 2017
-
Ingår i: Methods in Molecular Biology. - New York, NY : Humana Press Inc.. ; 1575, s. 175-187
-
Konferensbidrag (refereegranskat)abstract
- We describe a mass spectrometry-based approach for validation of antibody specificity. This method allows validation of antibodies or antibody fragments, against their endogenous targets. It can assess if the antibody is able to bind to its native antigen in cell lysates among thousands of other proteins, DNA, RNA, and other cellular components. In addition, it identifies other proteins the antibody is able to immunoprecipitate allowing for the assessment of antibody specificity and selectivity. This method is easily scalable, adaptable to different cell lines and conditions and has been shown to be reproducible between multiple laboratories.
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