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Träfflista för sökning "((WFRF:(Murphy J)) srt2:(2000-2004)) srt2:(2003)"

Sökning: ((WFRF:(Murphy J)) srt2:(2000-2004)) > (2003)

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  • Balan, S, et al. (författare)
  • Metal chelate affinity precipitation of RNA and purification of plasmid DNA
  • 2003
  • Ingår i: Biotechnology Letters. - 1573-6776. ; 25:13, s. 1111-1116
  • Tidskriftsartikel (refereegranskat)abstract
    • The affinity of metal chelates for amino acids, such as histidine, is widely used in purifying proteins, most notably through six-histidine `tails'. We have found that metal affinity interactions can also be applied to separation of single-stranded nucleic acids through interactions involving exposed purines. Here we describe a metal affinity precipitation method to resolve RNA from linear and plasmid DNA. A copper-charged copolymer of N-isopropyl acrylamide (NIPAM) and vinyl imidazole (VI) is used to purify plasmid from an alkaline lysate of E. coli. The NIPAM units confer reversible solubility on the copolymer while the imidazole chelates metal ions in a manner accessible to interaction with soluble ligands. RNA was separated from the plasmid by precipitation along with the polymer in the presence of 800 mM NaCl. Bound RNA could be recovered by elution with imidazole and separated from copolymer by a second precipitation step. RNA binding showed a strong dependence on temperature and on the type of buffer used.
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  • Thomson, Douglas W., et al. (författare)
  • Efficient Route to the Pineal Hormone Melatonin by Radical-Based Indole Synthesis
  • 2003
  • Ingår i: Synthetic Communications. - 0039-7911 .- 1532-2432. ; 33:20, s. 3631-3641
  • Tidskriftsartikel (refereegranskat)abstract
    • The hormone melatonin, which is known to have a range of important biological effects, has been prepared in a high-yielding route that features formation of the indole nucleus by radical cyclization. Mediation of the radical cyclization by tristrimethylsilylsilane (TTMSS) is more efficient than by N-ethylpiperidine hypophosphite.
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  • Williams, N M, et al. (författare)
  • A systematic genomewide linkage study in 353 sib pairs with schizophrenia.
  • 2003
  • Ingår i: American Journal of Human Genetics. - : Elsevier BV. - 0002-9297 .- 1537-6605. ; 73:6, s. 1355-1367
  • Tidskriftsartikel (refereegranskat)abstract
    • We undertook a genomewide linkage study in a total of 353 affected sib pairs (ASPs) with schizophrenia. Our sample consisted of 179 ASPs from the United Kingdom, 134 from Sweden, and 40 from the United States. We typed 372 microsatellite markers at approximately 10-cM intervals. Our strongest finding was a LOD score of 3.87 on chromosome 10q25.3-q26.3, with positive results being contributed by all three samples and a LOD-1 interval of 15 cM. This finding achieved genomewide significance (P<.05), on the basis of simulation studies. We also found two regions, 17p11.2-q25.1 (maximum LOD score [MLS] = 3.35) and 22q11 (MLS = 2.29), in which the evidence for linkage was highly suggestive. Linkage to all of these regions has been supported by other studies. Moreover, we found strong evidence for linkage (genomewide P<.02) to 17p11.2-q25.1 in a single pedigree with schizophrenia. In our view, the evidence is now sufficiently compelling to undertake detailed mapping studies of these three regions.
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  • Resultat 1-6 av 6

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