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| 2. |
- Abrahamson, Magnus
(författare)
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Cystatins
- 1994
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Ingår i: Methods in Enzymology. - : Elsevier. - 0076-6879. ; 244, s. 685-700
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Tidskriftsartikel (refereegranskat)
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| 3. |
- Abrahamson, Magnus, et al.
(författare)
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Increased body temperature accelerates aggregation of the (Leu-68–>Gln) mutant cystatin C, the amyloid-forming protein in hereditary cystatin C amyloid angiopathy
- 1994
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Ingår i: Proceedings of the National Academy of Sciences. - 1091-6490. ; 91:4, s. 1416-1420
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Tidskriftsartikel (refereegranskat)abstract
- Hereditary cystatin C amyloid angiopathy is a dominantly inherited disorder, characterized by dementia, paralysis, and death from cerebral hemorrhage in early adult life. A variant of the cysteine proteinase inhibitor, cystatin C, is deposited as amyloid in the tissues of the patients and their spinal-fluid level of cystatin C is abnormally low. The disease-associated Leu-68-->Gln mutant (L68Q) cystatin C has been produced in an Escherichia coli expression system and isolated by use of denaturing buffers, immunosorption, and gel filtration. Parallel physicochemical and functional investigations of L68Q-cystatin C and wild-type cystatin C revealed that both proteins effectively inhibit the cysteine proteinase cathepsin B (equilibrium constants for dissociation, 0.4 and 0.5 nM, respectively) but differ considerably in their tendency to dimerize and form aggregates. While wild-type cystatin C is monomeric and functionally active even after prolonged storage at elevated temperatures, L68Q-cystatin C starts to dimerize and lose biological activity immediately after it is transferred to a nondenaturing buffer. The dimerization of L68Q-cystatin C is highly temperature-dependent, with a rise in incubation temperature from 37 to 40 degrees C resulting in a 150% increase in dimerization rate. The aggregation at physiological concentrations is likewise increased at 40 compared to 37 degrees C, by approximately 60%. These properties of L68Q-cystatin C have bearing upon our understanding of the pathophysiological process of hereditary cystatin C amyloid angiopathy. They might also be of clinical relevance, since medical intervention to abort febrile periods of carriers of the disease trait may reduce the in vivo formation of L68Q-cystatin C aggregates.
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| 5. |
- Abrahamsson, Sven Olof, et al.
(författare)
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Variations in cellular proliferation and matrix synthesis in intrasynovial and extrasynovial tendons : An in vitro study in dogs
- 1994
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Ingår i: The Journal of Hand Surgery. - : Elsevier BV. - 0363-5023. ; 19:2, s. 259-265
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Tidskriftsartikel (refereegranskat)abstract
- Intrasynovial and extrasynovial flexor tendon grafts recently have been shown to have dissimilar patterns of cellular survival and host integration within the digital sheath. In an effort to determine if fundamental differences exist between these two types of tendons, we investigated the biochemical composition and cellular activity of intrasynovial and extrasynovial tendon segments of 12 adult mongrel dogs in short-term explant culture in MCDB 105 and in DMEM media. Proteoglycan, collagen and noncollagen protein synthesis and content and DNA synthesis were determined following culture in both media. Intrasynovial tendon segments cultured in MCDB 105 medium synthesized significantly less collagen, noncollagen protein, and DNA and had similar amounts of proteoglycans compared to extrasynovial tendons. Comparison of intrasynovial and extrasynovial tendon segment responses in DMEM medium showed that intrasynovial tendons synthesized more proteoglycan, protein, and DNA than they did in MCDB 105. Extrasynovial tendons had similar rates of matrix component and DNA synthesis in both media. Findings that the synthesis of matrix components and DNA between intrasynovial flexor and extrasynovial peroneal tendon segments differ significantly indicate that intrasynovial flexor tendons may be specially adapted to the nutritional milieu provided by an intrasynovial environment. These data are supported by the selective successful stimulation of fibrocartilaginous segments within intrasynovial flexor tendons in media favored for the culture of cartilaginous tissue.
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| 7. |
- Adalberth, Karin
(författare)
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Energi för att bygga bruka riva småhus, Bo92 : Den nya trästaden
- 1994
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- I denna rapport redovisas energiberäkningar för tre småhus på Bo 92 i Örebro. Syftet med rapporten är att beräkna och analysera det totala energibehovet och mängd byggnadsmaterial från 'vaggan till graven' för småhus. Med det totala energibehovet menas summan av energibehovet under produktion (materialtillverkning, materialtransporter och uppförande av byggnaden), förvaltning (brukande av byggnaden) samt destruktion (rivning och borttransport).
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| 8. |
- Adalberth, Karin
(författare)
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Energianvändning i småhus, Bo92. D. 1, Beräkningar : Den nya trästaden
- 1994
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- I denna rapport redovisas teoretiska beräkningar av energibehov till uppvärmning, tappvarmvatten och hushållselektricitet, energitransporter genom småhusens grunder och energiflöden genom köldbryggor. Beräkningarna omfattar 26 småhus på Bo 92 i Örebro med olika typer av grundläggning, värmeisolering, fönster, värme- och ventilationssystem. Beräkningarna har utförts med datorprogrammen ENORM, CRAWL, SLAB och HConP.
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| 9. |
- Adlercreutz, Patrick
(författare)
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Enzyme-catalysed lipid modification
- 1994
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Ingår i: Biotechnology and Genetic Engineering Reviews. - : Informa UK Limited. - 0264-8725 .- 2046-5556. ; 12:1, s. 231-254
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Forskningsöversikt (refereegranskat)
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| 10. |
- Adlercreutz, Patrick, et al.
(författare)
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Synthetic polymers as solubilizing vehicles for enzymes in water-poor media
- 1994
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Ingår i: Bioorganic and Medicinal Chemistry. - : Elsevier BV. - 0968-0896. ; 2:6, s. 529-533
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Forskningsöversikt (refereegranskat)abstract
- A recent method for exposing enzymes to organic solvents is reviewed. By complex formation between the enzyme and polymers that per se are soluble in organic solvents it is possible to disperse the enzyme in the organic medium in such a way that an optically transparent (in the visible region) solution is obtained. After reaction, the separation of the enzyme from the organic medium can be obtained simply by addition of water. The enzyme can be recovered from the water phase. Physicochemical studies have revealed that the enzyme is more stable in the complex-bound form.
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