Search: (LAR1:uu) pers:(Larsson Rolf) srt2:(2005-2009) pers:(Cabric Sanja)
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Carbachol-induced i...
Carbachol-induced in vitro secretion of certain human submandibular proteins investigated by mass-spectrometry.
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Cabras, Tiziana (author)
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Castagnola, Massimo (author)
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Inzitari, Rosanna (author)
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- Ekström, Jörgen, 1944 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för farmakologi,Institute of Neuroscience and Physiology, Department of Pharmacology
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Isola, Michela (author)
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Riva, Alessandro (author)
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Messana, Irene (author)
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(creator_code:org_t)
- Elsevier BV, 2008
- 2008
- English.
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In: Archives of oral biology. - : Elsevier BV. - 1879-1506 .- 0003-9969. ; 53:11, s. 1077-83
- Related links:
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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Abstract
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- OBJECTIVE: To investigate protein content of saliva produced in vitro by samples of human submandibular gland following stimulation with the muscarinic agent carbachol. DESIGN: Tissue samples, obtained at surgery from seven patients and showing normal morphological appearance, were tested for 30 min: in absence of carbachol and atropine; in presence of carbachol (10 microM); in presence of carbachol (10 microM) and atropine (20 microM); or in presence of just atropine (20 microM). Medium was analysed by high-performance liquid chromatography-mass-spectrometry. Neither before nor during surgery were the patients exposed to drug treatments that were likely to influence the in vitro secretion. RESULTS: Proline-rich proteins (PRP)-1 and -3, peptide PC and PB, statherin, cystatins SN, S1 and S2 were invariably found in control gland tissue medium. Mean concentrations of these proteins/peptides in the medium were non-proportionally elevated following carbachol exposure to the gland tissues. Difference between basal release and carbachol-induced secretion achieved statistical significance as to all the proteins/peptides under study but for statherin. Atropine alone or atropine plus carbachol caused no significant changes compared to the basal release of proteins/peptides. CONCLUSIONS: In vitro studies on salivary glands make it possible to study protein secretion from individual glands and thus, to reveal the contribution of the various types of gland to protein/peptide content of whole saliva. The disproportional responses to carbachol may imply that the proteins/peptides are not confined to the same cells or to the same intracellular locations and are therefore not secreted as packages at parasympathetic cholinergic activity.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Farmakologi och toxikologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Pharmacology and Toxicology (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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