Procedures for Protoplast Isolation from Red Seaweeds.Demonstration on Gracilaria sordida and Gracilaria tenuistipitata.

• This procedure consists of four main steps. The precultivation of the plant material, the pretreatment of thetissue, the incubation of the tissue in an enzyme solution, and finally the purification of the protoplasts. Allsteps have influence on the final yield and viability of the protoplasts. To prevent contamination all work shouldbe done in a laminar flow hood. Sterilize all tools, andfilter all solutions through a 0,22 jJ.m sterile filter beforeuse. Use standard axenic procedures.By this method a protoplast yield of 1x1a6 to 1x107 per gram fresh weight has been obtained. These protoplastswere viable and divided up to three times while in culture. (Bjork et al. 1990)

Nyckelord

protoplast

algae

seaweed

gracilaria

Plant Physiology

växtfysiologi

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