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- Clo, E., et al.
(författare)
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Characterization of the Viral O-Glycopeptidome: a Novel Tool of Relevance for Vaccine Design and Serodiagnosis
- 2012
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Ingår i: Journal of Virology. - : American Society for Microbiology. - 0022-538X .- 1098-5514. ; 86:11, s. 6268-6278
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Tidskriftsartikel (refereegranskat)abstract
- Viral envelope proteins mediate interactions with host cells, leading to internalization and intracellular propagation. Envelope proteins are glycosylated and are known to serve important functions in masking host immunity to viral glycoproteins. However, the viral infectious cycle in cells may also lead to aberrant glycosylation that may elicit immunity. Our knowledge of immunity to aberrant viral glycans and glycoproteins is limited, potentially due to technical limitations in identifying immunogenic glycans and glycopeptide epitopes. This work describes three different complementary methods for high-throughput screening and identification of potential immunodominant O-glycopeptide epitopes on viral envelope glycoproteins: (i) on-chip enzymatic glycosylation of scan peptides, (ii) chemical glycopeptide microarray synthesis, and (iii) a one-bead-one-compound random glycopeptide library. We used herpes simplex virus type 2 (HSV-2) as a model system and identified a simple O-glycopeptide pan-epitope, (501)PPA(GalNAc)TAPG(507), on the mature gG-2 glycoprotein that was broadly recognized by IgG antibodies in HSV-2-infected individuals but not in HSV-1-infected or noninfected individuals. Serum reactivity to the extended sialyl-T glycoform was tolerated, suggesting that self glycans can participate in immune responses. The methods presented provide new insight into viral immunity and new targets for immunodiagnostic and therapeutic measures.
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- Schlatter, Nicola, 1985-, et al.
(författare)
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Radar interferometer calibration of the EISCAT Svalbard Radar and a additional receiver station
- 2013
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Ingår i: Journal of Atmospheric and Solar-Terrestrial Physics. - : Elsevier BV. - 1364-6826 .- 1879-1824. ; 105-106, s. 287-292
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Tidskriftsartikel (refereegranskat)abstract
- The EISCAT Svalbard Radar has two parabolic dishes. In order to attempt to implement radar aperture synthesis imaging methods three smaller, passive receive array antennas were built. Several science goals for this new receiver system exist, the primary of which is to study so called naturally enhanced ion acoustic lines. In order to compare radar aperture synthesis imaging results with measurements from optical imagers, calibration of the radar interferometer system is necessary. In this work we present the phase calibration of the EISCAT Svalbard interferometer including one array antenna. The calibration was done using the coherent scatter from satellites passing through the radar beam. Optical signatures of the satellite transits provide accurate position for the satellites. By using transits of a number of satellites sufficient for mapping the radar beam, the interferometric cross-phase was fitted within the radar beam. The calibration technique presented in this work will be applied to all antenna pairs of the antenna configuration for future interferometry studies.
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