| 1. |
- Apweiler, Rolf, et al.
(författare)
-
Approaching clinical proteomics : current state and future fields of application in cellular proteomics
- 2009
-
Ingår i: Cytometry. Part A : the journal of the International Society for Analytical Cytology. - : Wiley. - 1552-4922. ; 75A:10, s. 816-832
-
Forskningsöversikt (refereegranskat)abstract
- Recent developments in proteomics technology offer new opportunities for clinical applications in hospital or specialized laboratories including the identification of novel biomarkers, monitoring of disease, detecting adverse effects of drugs, and environmental hazards. Advanced spectrometry technologies and the development of new protein array formats have brought these analyses to a standard, which now has the potential to be used in clinical diagnostics. Besides standardization of methodologies and distribution of proteomic data into public databases, the nature of the human body fluid proteome with its high dynamic range in protein concentrations, its quantitation problems, and its extreme complexity present enormous challenges. Molecular cell biology (cytomics) with its link to proteomics is a new fast moving scientific field, which addresses functional cell analysis and bioinformatic approaches to search for novel cellular proteomic biomarkers or their release products into body fluids that provide better insight into the enormous biocomplexity of disease processes and are suitable for patient stratification, therapeutic monitoring, and prediction of prognosis. Experience from studies of in vitro diagnostics and especially in clinical chemistry showed that the majority of errors occurs in the preanalytical phase and the setup of the diagnostic strategy. This is also true for clinical proteomics where similar preanalytical variables such as inter- and intra-assay variability due to biological variations or proteolytical activities in the sample will most likely also influence the results of proteomics studies. However, before complex proteomic analysis can be introduced at a broader level into the clinic, standardization of the preanalytical phase including patient preparation, sample collection, sample preparation, sample storage, measurement, and data analysis is another issue which has to be improved. In this report, we discuss the recent advances and applications that fulfill the criteria for clinical proteomics with the focus on cellular proteomics (cytoproteomics) as related to preanalytical and analytical standardization and to quality control measures required for effective implementation of these technologies and analytes into routine laboratory testing to generate novel actionable health information. It will then be crucial to design and carry out clinical studies that can eventually identify novel clinical diagnostic strategies based on these techniques and validate their impact on clinical decision making.
|
|
| 2. |
- Apweiler, Rolf, et al.
(författare)
-
Approaching clinical proteomics : current state and future fields of application in fluid proteomics
- 2009
-
Ingår i: Clinical Chemistry and Laboratory Medicine. - 1434-6621 .- 1437-4331. ; 47:6, s. 724-744
-
Forskningsöversikt (refereegranskat)abstract
- The field of clinical proteomics offers opportunities to identify new disease biomarkers in body fluids, cells and tissues. These biomarkers can be used in clinical applications for diagnosis, stratification of patients for specific treatment, or therapy monitoring. New protein array formats and improved spectrometry technologies have brought these analyses to a level with potential for use in clinical diagnostics. The nature of the human body fluid proteome with its large dynamic range of protein concentrations presents problems with quantitation. The extreme complexity of the proteome in body fluids presents enormous challenges and requires the establishment of standard operating procedures for handling of specimens, increasing sensitivity for detection and bioinformatical tools for distribution of proteomic data into the public domain. From studies of in vitro diagnostics, especially in clinical chemistry, it is evident that most errors occur in the preanalytical phase and during implementation of the diagnostic strategy. This is also true for clinical proteomics, and especially for fluid proteomics because of the multiple pretreatment processes. These processes include depletion of high-abundance proteins from plasma or enrichment processes for urine where biological variation or differences in proteolytic activities in the sample along with preanalytical variables such as inter- and intra-assay variability will likely influence the results of proteomics studies. However, before proteomic analysis can be introduced at a broader level into the clinical setting, standardization of the preanalytical phase including patient preparation, sample collection, sample preparation, sample storage, measurement and data analysis needs to be improved. In this review, we discuss the recent technological advances and applications that fulfil the criteria for clinical proteomics, with the focus on fluid proteomics. These advances relate to preanalytical factors, analytical standardization and quality-control measures required for effective implementation into routine laboratory testing in order to generate clinically useful information. With new disease biomarker candidates, it will be crucial to design and perform clinical studies that can identify novel diagnostic strategies based on these techniques, and to validate their impact on clinical decision-making.
|
|
| 3. |
|
|
| 4. |
- Kessar, Alexandros, et al.
(författare)
-
Flow Measurements for Low Engine order Excitations in a High Pressure Turbine stage
- 2005
-
Konferensbidrag (refereegranskat)abstract
- This paper demonstrates and evaluates unique flow measurement results obtained in a high pressure test turbine, in order to analyze the effect of Low Engine Order (LEO) excitations. A stator was modified to induce LEO flow variations by either imposing a throat width variation or a blockage of the trailing edge cooling flow from some of the vanes. Laser Two Focus (L2F) and pressure probe measurements were performed at subsonic and transonic flow conditions, without and with the rotor installed and operated. Time resolved velocity data was obtained in front and inside of rotor passages covering the Low Engine Order variation period. The presented results evaluation focuses only on L2F measurements and show that the LEO variation of flow velocity and turbulence intensity is most visible in the gap between stator and rotor and at the first measurement location inside the rotor passage. The overall trend is that stator exit flow Mach numbers are higher behind the passages with smaller pitch and vice versa.
|
|
| 5. |
- Rapp, Markus, et al.
(författare)
-
Observations of positively charged nanoparticles in the nighttime polar mesosphere
- 2005
-
Ingår i: Geophysical Research Letters. - 0094-8276 .- 1944-8007. ; 32, s. L23821-
-
Tidskriftsartikel (refereegranskat)abstract
- We present results of in situ measurements of charged nanoparticles, electrons, and positive ions obtained during a sounding rocket flight in October 2004 from Kiruna, Sweden, under nighttime conditions. The particle measurement reveals positive charge signatures in the altitude range between 80 and 90 km corresponding to peak charge number densities of ∼100 e/cm3 at around 86 km. Aerodynamical analysis of the sampling efficiency of our instrument reveals that the particles must have been larger than 2 nm assuming spherical particles with a density of 3 g/cm3. The plasma environment of the observed particles is dominated by negative and positive ions, with only few free electrons. A calculation of the mean particle charge expected for particles in a plasma consisting of electrons and positive and negative ions shows that the presence of sufficiently heavy and numerous negative ions (i.e., m n > 300 amu and λ ≥ 50) can explain the observed positive particle charge.
|
|
| 6. |
- Richards, Stephen, et al.
(författare)
-
The genome of the model beetle and pest Tribolium castaneum.
- 2008
-
Ingår i: Nature. - 1476-4687. ; 452:7190, s. 949-55
-
Tidskriftsartikel (refereegranskat)abstract
- Tribolium castaneum is a representative of earth’s most numerous eukaryotic order, a powerful model organism for the study of generalized insect development, and also an important pest of stored agricultural products. We describe its genome sequence here. This omnivorous beetle has evolved an ability to interact with a diverse chemical environment as evidenced by large expansions in odorant and gustatory receptors, as well as p450 and other detoxification enzymes. Developmental patterns in Tribolium are more representative of other arthropods than those found in Drosophila, a fact represented in gene content and function. For one, Tribolium has retained more ancestral genes involved in cell-cell communication than Drosophila, and some are expressed in the growth zone crucial for axial elongation in short germ development. Systemic RNAi in T. castaneum appears to use mechanisms distinct from those found in C. elegans, but nevertheless offers similar power for the elucidation of gene function and identification of targets for selective insect control.
|
|
| 7. |
- Strelnikova, Irina, et al.
(författare)
-
Measurements of meteor smoke particles during the ECOMA-2006 campaign. 2 : Results
- 2009
-
Ingår i: Journal of Atmospheric and Solar-Terrestrial Physics. - : Elsevier BV. - 1364-6826 .- 1879-1824. ; 71:3-4, s. 486-496
-
Tidskriftsartikel (refereegranskat)abstract
- The first sounding rocket of the European ECOMA-project (ECOMA, Existence and Charge state Of Meteoric smoke particles in the middle Atmosphere) was launched on 8 September 2006. Measurements with a new particle detector described in the companion paper by Rapp and Strelnikova [2008. Measurements of meteor smoke particles during the ECOMA-2006 campaign: 1. Particle detection by active photoionization. Journal of Atmospheric and Solar-Terrestrial Physics, this issue, doi:10.1016/j.jastp.2008.06.002] clearly showed meteor smoke particle (MSP) signatures in both data channels. The data channels measure particles directly impacting on the detector electrode and photoelectrons from the particles actively created using ionization by the UV-photons of a xenon-flashlamp. Measured photoelectron currents resemble model expectations of the shape of the MSP layer almost perfectly, whereas derived number densities in the altitude range 60–90 km are larger than model results by about a factor of 5. Given the large uncertainties inherent to both model and the analysis of our measurements (e.g., the composition of the particles is not known and must be assumed) we consider this a satisfactory agreement and proof that MSPs do extend throughout the entire mesosphere as predicted by models. The measurements of direct particle impacts revealed a confined layer of negative charge between 80 and 90 km. This limited altitude range, however, is quantitatively shown to be the consequence of the aerodynamics of the rocket flight and does not have any geophysical origin. Measured charge signatures are consistent with expectations of particle charging given our own measurements of the background ionization. Unfortunately, however, a contamination of these measurements from triboelectric charging cannot be excluded at this stage
|
|
| 8. |
- Taussig, Michael J., et al.
(författare)
-
ProteomeBinders : planning a European resource of affinity reagents for analysis of the human proteome
- 2007
-
Ingår i: Nature Methods. - : Springer Science and Business Media LLC. - 1548-7091 .- 1548-7105. ; 4:1, s. 13-17
-
Tidskriftsartikel (refereegranskat)abstract
- ProteomeBinders is a new European consortium aiming to establish a comprehensive resource of well-characterized affinity reagents, including but not limited to antibodies, for analysis of the human proteome. Given the huge diversity of the proteome, the scale of the project is potentially immense but nevertheless feasible in the context of a pan-European or even worldwide coordination.
|
|
