| 1. |
- Brodelius, Peter, et al.
(författare)
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A Procedure for the Determination of Optimal Chitosan Concentrations for Elicitation of Cultured Plant Cells.
- 1989
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Ingår i: Phytochemistry. - 0031-9422 .- 1873-3700. ; 28:10, s. 2651-2654
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Tidskriftsartikel (refereegranskat)abstract
- An experimental method for determination of the optimum chitosan concentration for elicitation of plant cell suspension cultures is presented. The procedure, which is based on measurements of the conductivity of the culture medium after addition of chitosan, has been applied to suspension cultures of Nicotiana tabacum and Eschscholtzia californica. Increased conductivity of the medium (due to permeabilization of the cells) results in decreased secondary product formation and cell growth. Maximum product formation is observed for cells elicited with the highest chitosan concentration which does not affect membrane permeability.
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| 2. |
- Brodelius, Peter, et al.
(författare)
-
Permeabilization of Cultivated Plant Cells by Electroporation for Release of Intracellularly Stored Secondary Products
- 1988
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Ingår i: Plant Cell Reports. - 0721-7714 .- 1432-203X. ; 7:3, s. 186-188
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Tidskriftsartikel (refereegranskat)abstract
- Plant cell suspension cultures producing secondary metabolites have been permeabilized for product release by electroporation. The two cell cultures studied, i.e. Thalictrum rugosum and Chenopodium rubrum, require about 5 and 10 kV cm–1, respectively, for complete permeabilization (release of all the intracellularly stored product). The number of electrical pulses and capacitance used had a relatively limited effect on product release while the viability of the cells was strongly influenced by the latter. Conditions for complete product release resulted in total loss of viability of the cells after treatment. The release of product from immobilized cells was also achieved by electroporation. Cells entrapped in alginate required less voltage for permeabilization than free or agarose entrapped cells.
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| 3. |
- Funk, C, et al.
(författare)
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Increased Secondary Product Formation in Plant Cell Suspension Cultures after Treatment with a Yeast Carbohydrate (Elicitor)
- 1987
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Ingår i: Phytochemistry. - 0031-9422 .- 1873-3700. ; 26, s. 401-405
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Tidskriftsartikel (refereegranskat)abstract
- A carbohydrate fraction isolated from yeast extract by ethanolic precipitation was used as an elicitor to induce secondary product formation in plant cell suspension cultures. The elicitor preparation is effective in inducing glyceollin isomer synthesis (up to 200 μg glyceollin per g dry wt) in cells of Glycine max and enhancing berberine biosynthesis (up to four-fold) in cells of Thalictrum rugosum. The response of the cell cultures to the elicitor treatment is dependent on the amount of carbohydrate per unit of biomass and on the physiological state of the cells. Cells are optimally induced in late exponential or early stationary growth phases.
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| 4. |
- Gügler, K, et al.
(författare)
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Elicitor-induced Tyrosine Decarboxylase in Berberine Synthesizing Suspension Cultures of Thalictrum rugosum
- 1988
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Ingår i: European Journal of Biochemistry. - : Wiley. - 0014-2956 .- 1432-1033. ; 170:3, s. 661-666
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Tidskriftsartikel (refereegranskat)abstract
- Tyrosine decarboxylase (EC 4.1.1.25) was induced in suspension cultures of Thalictrum rugosum by treatment with a yeast glucan elicitor. Maximum induction was observed at a carbohydrate concentration of 0.4 mg/g fresh weight of cells and maximum enzyme activity was reached 20 h after addition of elicitor. The enzyme was inducible in late exponential and early stationary growth phases. A good correlation between induced tyrosine decarboxylase activity and berberine biosynthesis has been established. It is suggested that tyrosine decarboxylase may be a key enzyme between primary and secondary metabolisms in the biosynthesis of norlaudanosoline-derived alkaloids.
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