| 1. |
- Hu, LP, et al.
(författare)
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Regulation of lipolytic activity by long-chain acyl-coenzyme A in islets and adipocytes
- 2005
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Ingår i: American Journal of Physiology: Endocrinology and Metabolism. - : American Physiological Society. - 1522-1555 .- 0193-1849. ; 289:6, s. 1085-1092
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Tidskriftsartikel (refereegranskat)abstract
- Intracellular lipolysis is a major pathway of lipid metabolism that has roles, not only in the provision of free fatty acids as energy substrate, but also in intracellular signal transduction. The latter is likely to be particularly important in the regulation of insulin secretion from islet beta-cells. The mechanisms by which lipolysis is regulated in different tissues is, therefore, of considerable interest. Here, the effects of long-chain acyl-CoA esters (LC-CoA) on lipase activity in islets and adipocytes were compared. Palmitoyl-CoA (Pal-CoA, 1-10 mu M) stimulated lipase activity in islets from both normal and hormone-sensitive lipase (HSL)-null mice and in phosphatase-treated islets, indicating that the stimulatory effect was neither on HSL nor phosphorylation dependent. In contrast, we reproduced the previously published observations showing inhibition of HSL activity by LC-CoA in adipocytes. The inhibitory effect of LC-CoA on adipocyte HSL was dependent on phosphorylation and enhanced by acyl-CoA-binding protein (ACBP). In contrast, the stimulatory effect on islet lipase activity was blocked by ACBP, presumably due to binding and sequestration of LC-CoA. These data suggest the following intertissue relationship between islets and adipocytes with respect to fatty acid metabolism, LC-CoA signaling, and lipolysis. Elevated LC-CoA in islets stimulates lipolysis to generate a signal to increase insulin secretion, whereas elevated LC-CoA in adipocytes inhibits lipolysis. Together, these opposite actions of LC-CoA lower circulating fat by inhibiting its release from adipocytes and promoting fat storage via insulin action.
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| 2. |
- Li, N., et al.
(författare)
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Detection wavelengths and photocurrents of very long wavelength quantum-well infrared photodetectors
- 2005
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Ingår i: Infrared Physics and Technology. - : Elsevier BV. - 1350-4495. ; 47:1-2, s. 29-36
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Tidskriftsartikel (refereegranskat)abstract
- Based on detailed studies of the energy band structure and the optical transitions in very long wavelength (>14 μm) GaAs/AlGaAs quantum-well (QW) infrared photodetectors (QWIPs), we have built a practical QWIP model. We study the factors that determine photogenerated carriers and response wavelengths of photocurrents of very long wavelength QWIPs. The material structures of QWIPs are first characterized by the photoluminescence measurements (PL) at room temperature and 77 K respectively. We have found and confirmed a distinctive difference between photocurrent of QWIPs with only one confined state in the quantum well (QW) and those binding two confined states, which resulted in different dependence of detection wavelength on the quantum well width. Also, we have investigated the dependence of response wavelength on several other parameters for very long wavelength QWIPs, such as barrier width and Al mole fraction. By calculating the density of photogenerated carriers in the continuum above the energy barriers using the PL calibrated QWIP structures, we have demonstrated that due to the high sample quality, the photocarriers can be either in miniband states (Bloch states in the multiple quantum wells), or they transport from one quantum well to the next in the form of propagating waves. We have further calculated the densities of photocarriers in the QWIPs reported in the literature. It is shown that the Bloch wave boundary conditions are appropriate for QWIPs with narrow QWs, whereas propagating wave boundary conditions are appropriate for wide QWs.
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| 3. |
- Yan, H, et al.
(författare)
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Superior neovascularization and muscle regeneration in ischemic skeletal muscles following VEGF gene transfer by rAAV1 pseudotyped vectors
- 2005
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Ingår i: Biochemical and Biophysical Research Communications. - : Elsevier BV. - 1090-2104 .- 0006-291X. ; 336:1, s. 287-298
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Tidskriftsartikel (refereegranskat)abstract
- Recombinant adeno-associated virus serotype 2 (rAAV2) vector has been widely employed for gene therapy. Recent progress suggests that the new serotypes of AAV showed a better performance than did AAV2 in normal tissues. Here, we evaluate the potential role of human vascular endothelial growth factor (VEGF) gene transfer using rAAV vector pseudotyped with serotype I capsid proteins (rAAV1) in the treatment of muscle ischemia. In ischemic skeletal muscles, the rAAV1-LacZ vector allowed higher level, broader distribution, and long-lasting gene expression compared with the rAAV2-LacZ vector. Muscle VEGF165 production following the rAAV1-VEGF165 vector injection was 5-10 times higher than that following the rAAV2-VEGF165 vector injection. VEGF165 production mediated by the rAAV1-VEGF165 vector stimulated a large set of neovascularization with relatively mature vascular structures and enhanced muscle regeneration in the ischemic skeletal muscles. Thus, the rAAV1-NEGF165 vector mediated gene transfer may be a therapeutic approach to peripheral vascular diseases.
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