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Sökning: (WFRF:(Guseva Anna)) > (2020)

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1.
  • Beal, Jacob, et al. (författare)
  • Robust estimation of bacterial cell count from optical density
  • 2020
  • Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
  • Tidskriftsartikel (refereegranskat)abstract
    • Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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2.
  • Nauman, Farrukh, 1985, et al. (författare)
  • Energy transfers in magnetohydrodynamic shear turbulence
  • 2020
  • Ingår i: Journal of Physics: Conference Series. - : IOP Publishing. - 1742-6588 .- 1742-6596. ; 1522:1
  • Konferensbidrag (refereegranskat)abstract
    • Astrophysical flows span a broad range of scales and thus require sub-grid modeling. We compute the energy fluxes in the Fourier and physical space for the turbulent magnetized shearing box simulations with a weak net vertical magnetic flux. The energy fluxes are dominated by the injection term from the mid-to-large scale range due to the Reynolds and Maxwell stresses in both cases while the small scales are dominated by dissipation. Since the nature of turbulent cascades requires a clear separation of scales, our work is unable to address these questions conclusively. But because our work is the first of its nature in trying to connect the Fourier and physical space energy flux behavior, we are able to make connections between the two profiles.
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