Genomic organization of profilin-III and evidence for a transcript expressed exclusively in testis.

• Profilins are small, widely expressed actin binding proteins, thought to be key regulators of actin dynamics in living cells. So far, three profilin-genes have been described: profilin-I (PFN1), profilin-II (PFN2) with two splice variants and the recently identified profilin-III (PFN3). Here we describe the genomic organization of the genes encoding human and mouse profilin-III. Both are single exon genes and lie in close vicinity to the renal sodium-phosphate transport gene 2 (SLC34A1, NPT2) which is highly expressed in kidney. Northern hybridization to rat tissues has previously demonstrated expression of an approximately 4.5 kb long profilin-III mRNA transcript in kidney and a mRNA transcript of approximately 1 kb in length in testis. Here we show that mouse profilin-III expression is restricted to testis and that the 4.2 kb profilin-III mRNA in kidney is the result of a slc34a1 transcript which includes the antisense profilin-III open reading frame in its 3prime prime or minute-untranslated region. Finally, we demonstrate by in situ hybridization that profilin-III mRNA is localized to cells in the late stage of spermatogenesis.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Cancer och onkologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Cancer and Oncology (hsv//eng)

Nyckelord

In Situ Hybridization

Human

Gene Expression

Kidney : metabolism

Mice

Male

Mice Inbred Strains

Microfilament Proteins : genetics

Molecular Sequence Data

Protein Isoforms : genetics

RNA Messenger : genetics : metabolism

Rats

Reverse Transcriptase Polymerase Chain Reaction

Sequence Alignment

Sequence Homology Amino Acid

Sequence Analysis DNA

Transcription Genetic

Testis : metabolism

DNA : chemistry : genetics

DNA Complementary : chemistry : genetics

Blotting Northern

Base Sequence

Animal

Amino Acid Sequence

Publikations- och innehållstyp

art (ämneskategori)

ref (ämneskategori)