| 1. |
- Karlsson, Caroline, et al.
(författare)
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5-Hydroxytryptamine contracts human uterine artery smooth muscle predominantly via 5-HT2 receptors
- 1997
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Ingår i: Human Reproduction. - 0268-1161. ; 12:2, s. 361-367
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Tidskriftsartikel (refereegranskat)abstract
- Serotonergic receptors were classified in the isolated human uterine artery with intact endothelium, using agonists and antagonists for 5-hydroxytryptamine (5-HT) receptors. The efficacy for different agonists rated: alpha-methyl-5-HT (5-HT2) = 5-HT (non-selective) = 2-methyl-5-HT (5-HT3) >> sumatriptan (5-HT1), and the potency as: sumatriptan = 5-HT > 5-HT > alpha-methyl-5-HT > 2-methyl-5-HT. The contractile effects of 5-HT and alpha-methyl-5-HT were antagonized by the 5-HT2 receptor antagonist ketanserin and the non-selective antagonist methiothepin. The efficacy of sumatriptan was comparatively low. No interaction was encountered between 2-methyl-5-HT and MDL72222, suggesting an absence of 5-HT3 receptors. The results indicate that the contractile serotonergic receptor population in the human uterine artery mainly comprises 5-HT2 receptors, although a minor contribution of contractile 5-HT1 receptors cannot be excluded.
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| 2. |
- Karlsson, Caroline, et al.
(författare)
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Characterization of 5-hydroxytryptamine receptors mediating circular smooth muscle contraction in the human umbilical artery
- 1999
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Ingår i: Gynecologic and Obstetric Investigation. - : S. Karger AG. - 1423-002X .- 0378-7346. ; 47:2, s. 102-107
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Tidskriftsartikel (refereegranskat)abstract
- The study was performed to characterize pharmacologically the contractile 5-hydroxytryptamine (5-HT) receptors in the circular smooth muscle of the isolated human umbilical artery. Effects of agonists and antagonists for different 5-HT receptor subtypes were studied in intact endothelium vessel segments. All agonists induced concentration-dependent circular smooth muscle contractions. The potency was in declining order 5-HT > alpha-methyl-5-HT > sumatriptan >/= 2-methyl-5-HT. The effects of 5-HT and alpha-methyl-5-HT were antagonized by ketanserin, as well as methiothepin. The contractile effect of sumatriptan was antagonized by methiothepin but not by ketanserin. The 5-HT3 receptor antagonist, MDL 72222, did not affect the contraction by any of the agonists, including 2-methyl-5-HT. It is concluded that the 5-HT-induced contraction in the circular smooth muscle of the human umbilical artery seems to be mediated by a mixed population of 5-HT1-like receptors and 5-HT2 receptors.
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| 3. |
- Karlsson, Caroline
(författare)
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Direct and endothelium-linked serotonergic control of vascular tone in human uterine and umbilical arteries
- 1998
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- The serotonergic vascular control was investigated by in vitro pharmacology in the human uterine and umbilical arteries. The localisation of immunoreactive (IR) neuropeptide Y (NPY) and the influence of NPY on the adrenergic vascular effects in the human uterine artery were also studied. NPY-IR nerve fibres were observed predominantly at the media-adventitial border of the uterine artery. About 50% of the NPY-IR nerve fibres also contained tyrosine hydroxylase-IR. NPY potentiated the noradrenergic contraction in the uterine artery but had no effect per se. The receptors mediating the serotonergic contractile effect in the human uterine artery were of the 5-hydroxytryptamine (5-HT)2-subtype with a minor contribution of 5-HT1 receptors. The endothelium of the uterine artery secreted a vasodilating substance in response to 5-HT. This substance seemed to be a prostanoid, probably prostacylin. The endothelium-linked effect appeared to be mediated by 5-HT1 receptors. In the human umbilical artery 5-HT mediated a contraction via a mixed population of 5-HT1 and 5-HT2 receptors. The endothelium secreted a vasoconstrictive substance in response to 5-HT. This endothelium-linked effect also appeared to be mediated by receptors of the 5-HT1 subtype. It was not possible to detect the substance released by the endothelium of the umbilical artery in response to 5-HT, although endothelin could not be excluded.
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