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- Birney, Ewan, et al.
(författare)
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Identification and analysis of functional elements in 1% of the human genome by the ENCODE pilot project
- 2007
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Ingår i: Nature. - : Springer Science and Business Media LLC. - 0028-0836 .- 1476-4687. ; 447:7146, s. 799-816
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Tidskriftsartikel (refereegranskat)abstract
- We report the generation and analysis of functional data from multiple, diverse experiments performed on a targeted 1% of the human genome as part of the pilot phase of the ENCODE Project. These data have been further integrated and augmented by a number of evolutionary and computational analyses. Together, our results advance the collective knowledge about human genome function in several major areas. First, our studies provide convincing evidence that the genome is pervasively transcribed, such that the majority of its bases can be found in primary transcripts, including non-protein-coding transcripts, and those that extensively overlap one another. Second, systematic examination of transcriptional regulation has yielded new understanding about transcription start sites, including their relationship to specific regulatory sequences and features of chromatin accessibility and histone modification. Third, a more sophisticated view of chromatin structure has emerged, including its inter-relationship with DNA replication and transcriptional regulation. Finally, integration of these new sources of information, in particular with respect to mammalian evolution based on inter- and intra-species sequence comparisons, has yielded new mechanistic and evolutionary insights concerning the functional landscape of the human genome. Together, these studies are defining a path for pursuit of a more comprehensive characterization of human genome function.
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- Myslivets, E., et al.
(författare)
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1.56-μs continuously tunable parametric delay line for a 40-Gb/s signal
- 2009
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Ingår i: Optics Express. - 1094-4087 .- 1094-4087. ; 17:14, s. 11958-11964
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Tidskriftsartikel (refereegranskat)abstract
- Experimental demonstration of an all-fiber, all-optical continuously tunable delay line is reported. The 1.56-mu s delay with a record 62,400 time-delay bit-rate product was characterized for a 40-Gbps data channel. The result was enabled by parametric dispersion compensation with cascaded triple-conversion in highly-nonlinear fiber capable of continuous tuning over 39.5 nm.
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| 6. |
- Dogiel, V. A., et al.
(författare)
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In-situ acceleration of subrelativistic electrons in the Coma halo and the halo’s influence on the Sunyaev-Zeldovich effect
- 2007
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Ingår i: Astronomy and Astrophysics. - : EDP Sciences. - 0004-6361 .- 1432-0746. ; 461:2, s. 433-443
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Tidskriftsartikel (refereegranskat)abstract
- Aims. The stochastic acceleration of subrelativistic electrons from a background plasma is studied in order to find a possible explanation of the hard X-ray emission detected from the Coma cluster. Methods. We calculate the necessary energy supply as a function of the plasma temperature and of the electron energy, and we show that, for the same value of the hard X-ray flux, the energy supply changes gradually from its high value for the case when emitting particle are non-thermal to lower values whenthe electrons are thermal. The kinetic equations we use include terms describing particle thermalization as well as momentum diffusion due to the Fermi IIacceleration. Results. We show that the temporal evolution of the particle distribution function has, at its final stationary stage, a rather specific form. This distribution function cannot be described by simple exponential or power-law expressions. A broad transfer region is formed by Coulomb collisions at energies betweenthe Maxwellian and power-law parts of the distribution functions. In this region the radiative lifetime of a single quasi-thermal electron differs greatly from thelifetime of the distribution function as a whole. For a plasma temperature of 8 keV, the particles emitting bremsstrahlung at 20-80 keV lie in this quasi-thermal regime. We show that the energy supply required by quasi-thermal electrons to produce the observed hard X-ray flux from Coma is one or two orders ofmagnitude smaller than the value derived from the assumption of a nonthermal origin of the emitting particles. This result may solve the problem of rapid cluster overheating by nonthermal electrons raised by Petrosian (2001): while Petrosian's estimates are correct for nonthermal particles, they are inapplicablein the quasi-thermal range. We finally analyze the change in Coma's Sunyaev-Zeldovich effect caused by the implied distortions of the Maxwellian spectrumof electrons, and we show that evidence for the acceleration of subrelativistic electrons can, in principle, be derived from detailed spectral measurements.
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| 7. |
- Koliwad, S. K., et al.
(författare)
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Angiopoietin-like 4 (ANGPTL4, fasting-induced adipose factor) is a direct glucocorticoid receptor target and participates in glucocorticoid-regulated triglyceride metabolism
- 2009
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Ingår i: J Biol Chem. - 1083-351X .- 1083-351X. ; 284:38, s. 25593-601
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Tidskriftsartikel (refereegranskat)abstract
- Glucocorticoids are important regulators of lipid homeostasis, and chronically elevated glucocorticoid levels induce hypertriglyceridemia, hepatic steatosis, and visceral obesity. The occupied glucocorticoid receptor (GR) is a transcription factor. However, those genes regulating lipid metabolism under GR control are not fully known. Angiopoietin-like 4 (ANGPTL4, fasting-induced adipose factor), a protein inhibitor of lipoprotein lipase, is synthesized and secreted during fasting, when circulating glucocorticoid levels are physiologically increased. We therefore tested whether the ANGPTL4 gene (Angptl4) is transcriptionally controlled by GR. We show that treatment with the synthetic glucocorticoid dexamethasone increased Angptl4 mRNA levels in primary hepatocytes and adipocytes (2-3-fold) and in the livers and white adipose tissue of mice (approximately 4-fold). We tested the mechanism of this increase in H4IIE hepatoma cells and found that dexamethasone treatment increased the transcriptional rate of Angptl4. Using bioinformatics and chromatin immunoprecipitation, we identified a GR binding site within the rat Angptl4 sequence. A reporter plasmid containing this site was markedly activated by dexamethasone, indicative of a functional glucocorticoid response element. Dexamethasone treatment also increased histone H4 acetylation and DNase I accessibility in genomic regions near this site, further supporting that it is a glucocorticoid response element. Glucocorticoids promote the flux of triglycerides from white adipose tissue to liver. We found that mice lacking ANGPTL4 (Angptl4(-/-)) had reductions in dexamethasone-induced hypertriglyceridemia and hepatic steatosis, suggesting that ANGPTL4 is required for this flux. Overall, we establish that ANGPTL4 is a direct GR target that participates in glucocorticoid-regulated triglyceride metabolism.
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