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- Szymanski, J. J., et al.
(författare)
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MEGA : A search for the decay mu –> e gamma
- 1994
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Ingår i: Intersections between particle and nuclear physics. Proceedings, 5th Conference, St. Petersburg, USA, May 31-June 6, 1994. ; , s. 789-792
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Konferensbidrag (refereegranskat)
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- Birnir, Bryndis, et al.
(författare)
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Expression and characterization of the intestinal Na+/glucose cotransporter in COS-7 cells.
- 1990
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Ingår i: Biochimica et Biophysica Acta. - 0006-3002 .- 1878-2434. ; 1048:1, s. 100-4
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Tidskriftsartikel (refereegranskat)abstract
- Cells derived from the simian kidney, COS-7 cells, were transfected with a eucaryotic expression vector (pEUK-C1) containing the clone for the rabbit intestinal Na+/glucose cotransporter. Expression was monitored after transfection with lipofectin by measuring the initial rate of alpha-methylglucopyranoside (MeGlc) uptake. Cells transfected with vector containing the cDNA for the Na+/glucose cotransporter expressed Na(+)-dependent MeGlc transport. Neither control cells nor cells transfected with vector lacking cloned cDNA expressed the cotransporter. Na(+)-dependent MeGlc uptake into transfected cells was saturable (Km 150 microM), phlorizin-sensitive (Ki 11 microM), and inhibited by sugar analogs (D-glucose greater than MeGlc greater than D-galactose greater than 3-O-methyl-D-glucoside greater than D-allose much greater than L-glucose). Europium was able to mimic Na+ in driving MeGIC uptake. Finally, tunicamycin, an inhibitor of asparagine-linked glycosylation, inhibited the expression of Na(+)-dependent MeGlc transport 80%. We conclude that the rabbit intestinal Na+/glucose cotransporter expressed in COS-7 cell exhibits very similar kinetic properties to that in the native brush border and to that expressed in Xenopus oocytes. In addition, N-linked glycosylation appears to be important for functional expression of this membrane protein.
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4. |
- HUGHES, JR, et al.
(författare)
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LIFETIME MEASUREMENT IN EXCITED AND YRAST SUPERDEFORMED BANDS IN HG-194
- 1994
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Ingår i: Physical Review Letters. - 0031-9007 .- 1079-7114. ; 72:6, s. 824-827
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Tidskriftsartikel (refereegranskat)abstract
- Nuclear level lifetimes have been measured in two superdeformed bands in Hg-194 using the Doppler-shift attenuation method. Average transition quadrupole moments derived from the life-times of an excited and yrast superdeformed bands are Q(t) = 17.6(30) and 17.2(20) eb, respectively. The Doppler shifts of the excited band relative to the yrast band indicate a slight difference in quadrupole moments [+4(5)%], assuming similar side feeding. These results imply that the second well is stable and rigid with respect to the particle excitation responsible for this excited band.
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5. |
- Ryu, U, et al.
(författare)
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An efficient method of implementing the horizontal recurrence relation in the evaluation of electron repulsion integrals using Cartesian Gaussian functios
- 1991
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Ingår i: Chemical Physics Letters. - : Elsevier BV. - 0009-2614 .- 1873-4448. ; 185:5-6, s. 562-568
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Tidskriftsartikel (refereegranskat)abstract
- The tree search problem is considered for optimizing the horizontal recurrence relation step, which is a three-dimensional recurrence relation used in generating two-electron integrals. By eliminating redundant work, it is possible to achieve 13%, 25%, 38% and 44% savings in floating point operations and 21%, 34%, 46% and 53% reductions in memory requirements for the (ff\textbackslashff), (gg\textbackslashgg), (hh\textbackslashhh) and (ii\textbackslashii) cases, respectively. These savings and reductions will lead to quite efficient two-electron integral and derivative programs when high angular momentum functions are included.
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