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- Chumnarnsilpa, Sakesit, 1967-, et al.
(författare)
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Calcium ion exchange in crystalline gelsolin
- 2006
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Ingår i: Journal of Molecular Biology. - England : Academic Press. - 0022-2836 .- 1089-8638. ; 357:3, s. 773-782
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Tidskriftsartikel (refereegranskat)abstract
- Gelsolin is a calcium and pH-sensitive modulator of actin filament length. Here, we use X-ray crystallography to examine the extraction and exchange of calcium ions from their binding sites in different crystalline forms of the activated N and C-terminal halves of gelsolin, G1-G3 and G4-G6, respectively. We demonstrate that the combination of calcium and low pH activating conditions do not induce conformational changes in G4-G6 beyond those elicited by calcium alone. EGTA is able to remove calcium ions bound to the type I and type II metal ion-binding sites in G4-G6. Constrained by crystal contacts and stabilized by interdomain interaction surfaces, the gross structure of calcium-depleted G4-G6 remains that of the activated form. However, high-resolution details of changes in the ion-binding sites may represent the initial steps toward restoration of the arrangement of domains found in the calcium-free inactive form of gelsolin in solution. Furthermore, bathing crystals with the trivalent calcium ion mimic, Tb3+, results in anomalous scattering data that permit unequivocal localization of terbium ions in each of the proposed type I and type II ion-binding sites of both halves of gelsolin. In contrast to predictions based on solution studies, we find that no calcium ion is immune to exchange.
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| 2. |
- Holmberg, Bo, et al.
(författare)
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Adab and Arabic Literature
- 2006
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Ingår i: Literary History: Towards a Global Perspective. Volume 1: Notions of Literature Across Times and Cultures.
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Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
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| 3. |
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| 4. |
- Lindberg, Anne, et al.
(författare)
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Prevalence and underdiagnosis of COPD by disease severity and the attributable fraction of smoking Report from the Obstructive Lung Disease in Northern Sweden Studies.
- 2006
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Ingår i: Respiratory Medicine. - : Elsevier BV. - 0954-6111 .- 1532-3064. ; 100:2, s. 264-272
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Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND: There is a lack of epidemiological data on COPD by disease severity. We have estimated the prevalence and underdiagnosis of COPD by disease severity defined by the British Thoracic Society (BTS) and Global Initiative for Chronic Obstructive Lung Disease (GOLD) guidelines. The impact of smoking was evaluated by the population attributable fraction of smoking in COPD. METHODS: A random sample of 1500 responders of the third postal survey performed in 1996 of the Obstructive Lung Disease in Northern Sweden (OLIN) Studies' first cohort (6610 subjects recruited in 1985) were invited to structured interview and spirometry. One thousand two hundred and thirty-seven subjects (82%) performed spirometry. RESULTS: The prevalence of mild BTS-COPD was 5.3%, moderate 2.2%, and severe 0.6% (GOLD-COPD: mild 8.2%, moderate 5.3%, severe 0.7%, and very severe 0.1%). All subjects with severe COPD were symptomatic, corresponding figures among mild COPD were 88% and 70% (BTS and GOLD), Subjects with severe BTS-COPD reported a physician-diagnosis consistent with COPD in 50% of cases, in mild BTS-COPD 19%, while in mild GOLD-COPD only 5% of cases. The major risk factors, age and smoking, had a synergistic effect on the COPD-prevalence. The Odds Ratio (OR) for having COPD among smokers aged 76-77 years was 59 and 34 (BTS and GOLD) when non-smokers aged 46-47 was used as reference population. CONCLUSIONS: Most subjects with COPD have a mild disease. The underdiagnosis is related to disease-severity. Though being symptomatic, only a half of the subjects with severe COPD are properly labelled. Smoking and increasing age were the major risk factors and acted synergistic.
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| 5. |
- Lindberg, Anne, et al.
(författare)
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Seven-year cumulative incidence of COPD in an age-stratified general population sample.
- 2006
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Ingår i: Chest. - : Elsevier BV. - 0012-3692 .- 1931-3543. ; 129:4, s. 879-885
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Tidskriftsartikel (refereegranskat)abstract
- AIM: To estimate the cumulative incidence of COPD and risk factors related to the development of COPD, including evaluation of the relationship between Global Initiative for Chronic Obstructive Lung Disease (GOLD) stage 0 (ie, respiratory symptoms and normal lung function) and the development of COPD, in an age-stratified general population sample of middle-aged and elderly individuals. METHOD: The third survey of the Obstructive Lung Disease in Northern Sweden studies cohort I (three age strata born in 1919 to 1920, 1934 to 1935, and 1949 to 1950) was performed in 1996, and 5,189 subjects (88%) responded to the postal questionnaire. Of the responders, a random sample (1,500 subjects) was invited to an examination in 1996 and in 2003. A total of 963 subjects performed spirometry on both occasions. COPD was defined according to the spirometric criteria of the GOLD. Two levels of disease severity, grade I and higher (GOLD criteria, FEV(1)/FVC ratio of < 0.70) and also grade II and higher (GOLD II criteria, FEV(1)/FVC ratio of < 0.70 and FEV(1) <80% predicted). RESULTS: The 7-year cumulative incidence of COPD was 11.0% and 4.9%, respectively, according to GOLD and GOLD II, and was significantly related to smoking (smokers, 18.8% and 10.6%, respectively; ex-smokers, 10.5% and 5.2%, respectively; non-smokers, 7.6% and 1.6%, respectively). Incident COPD according to GOLD, but not according to GOLD II, was significantly associated with increasing age. Most respiratory symptoms at study entry were markers of increased risk for incident COPD when analyzed in a multivariate model adjusting for confounders. CONCLUSION: The GOLD criteria yielded a higher cumulative incidence (11.0%) compared to the GOLD II (4.9%). Smoking, but not gender, was associated with incident COPD. Most respiratory symptoms at the beginning of the observation period marked an increased risk for developing COPD, thus the classification GOLD stage 0 seems relevant among middle-aged and elderly persons.
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| 8. |
- Lindberg, Bo, 1946
(författare)
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Den lärda kulturen
- 2006
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Ingår i: Signums svenska kulturhistoria: Frihetstiden. - 9187896788 ; , s. 97-142
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Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
- Artikeln skildrar den lärda kulturen viduniveristet och akademier i frihetstidens Sverige. Tyngdpunkten ligger på universiteten och vardagsvillkoren där: undervisnig, förfsörjning, disputationer, publicering m.m.
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| 9. |
- Lindberg, Bo, et al.
(författare)
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Modeling of Cherenkov light emission from BWR nuclear fuel with missing or substituted rods
- 2006
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Ingår i: IAEA Symposium on International Safeguards: Addressing Verification Challenges.
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Konferensbidrag (populärvet., debatt m.m.)abstract
- Computer simulations of Cherenkov glow from spent nuclear fuel were carried out. Spent nuclear fuel in storage ponds are verified with the help of the Cherenkov viewing device (CVD) and the Digital Cherenkov viewing device (DCVD). The instruments image the Cherenkov glow generated by gamma ray emissions from spent fuel into the water. An attempt to build a realistic digital model of the DCVD image containing partial-length, missing, and substituted rods was made to see if the effects of the deviations from normal can be predicted. It was concluded that partial-length or missing rods in the model was in good agreement with measured data, but replaced rods in the model showed a weaker attenuation of the Cherenkov glow than the observed DCVD images.
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