| 1. |
- Håkansson, H., et al.
(författare)
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In vivo and in vitro toxicity of fractionated fish lipids, with particular regard to their content of chlorinated organic compounds
- 1991
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Ingår i: Pharmacology and Toxicology. - : Wiley. - 0901-9928 .- 1600-0773. ; 69:6, s. 459-471
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Tidskriftsartikel (refereegranskat)abstract
- Six different lipid matrices (the intact lipid (IL), four lipid fractions with different polarity, and the free fatty acids (FFAs) obtained by hydrolysis of the triacylglycerol (TAG) containing fraction) were obtained from salmon (Salmo salar) and eel (Anguilla anguilla), each collected at a contaminated and a comparatively uncontaminated catch site along the coast of Scandinavia. The lipid matrices were studied in toxicological test systems representing various biological functions of different organ systems from several species and trophic levels. The results were evaluated with particular respect to the concentrations of extractable organically bound chlorine (EOCl) in the matrices tested. In some test systems, the specimens with a higher EOCl concentration appeared to be more toxic. For example, the TAG containing fraction (F2) from Idefjord eel, having a higher EOCl content than F2 from Oslofjord eel, reduced the number and hatchability of eggs laid by zebrafish. Both IL and F2 of Idefjord eel increased mortality and reduced the oxygen/nitrogen-ratio in blue mussels. Non-polar compounds (F1) from Bothnian Sea salmon induced 7-ethoxyresurofin O-deethylase (EROD) activity in rainbow trout hepatocytes, whereas F1 from Senja salmon did not. F1 from Bothnian Sea salmon also reduced the number of T-cells in foetal mouse thymus anlagen in vitro compared with the cell number in anlagen exposed to F1 from Senja salmon. A positive correlation between EOCl concentration and test response was found for EROD activity in rainbow trout hepatocytes and for ATP-leakage in Erlich ascites tumour cells when testing the phospolipid containing fraction (F4). However, in most test systems the fish oils, irrespective of EOCl content, were of low toxicity, and the observed effects need to be verified in future studies.
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| 2. |
- Balbin, Milagros, et al.
(författare)
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A sequence variation in the human cystatin D gene resulting in an amino acid (Cys/Arg) polymorphism at the protein level
- 1993
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Ingår i: Human Genetics. - 1432-1203. ; 90:6, s. 668-669
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Tidskriftsartikel (refereegranskat)abstract
- A polymorphism in the coding region of the human cystatin D gene has been detected by direct sequencing of amplified DNA from different individuals. The variation, resulting from a T/C transition in exon 1 of the gene, causes an amino acid variation, Cys/Arg, at the protein level. An allele-specific oligonucleotide hybridization assay was developed and used to demonstrate this polymorphism in the population. The deduced frequencies were 0.55 and 0.45 for the Cys and Arg variant-encoding alleles, respectively.
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| 3. |
- Freije, José P, et al.
(författare)
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Human cystatin D: cDNA cloning, characterization of the E. coli expressed inhibitor, and identification of the native protein in saliva
- 1993
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Ingår i: Journal of Biological Chemistry. - 1083-351X. ; 268:21, s. 15737-15744
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Tidskriftsartikel (refereegranskat)abstract
- A cDNA coding for cystatin D, a human member of the cystatin protein family, has been cloned after specific amplification of reverse- transcribed parotid gland RNA. After replacing the segment encoding the putative 20-residue signal peptide with one encoding the Escherichia coli OmpA leader sequence, the cDNA was expressed in E. coli. The isolated recombinant protein exhibited Ki values of 1.2 nM and > 1 microM for papain and cathepsin B, respectively. An antiserum raised against recombinant cystatin D recognized a protein in human saliva with electrophoretical mobility identical to that of the recombinant protein. Immunoenzymatic analysis revealed that this cysteine proteinase inhibitor is present in human saliva and tears at concentrations of 3.8 and 0.5 mg/liter, respectively, while it was not detected in seminal plasma, blood plasma, milk, or cerebrospinal fluid. Cystatin D purified from human saliva by immunosorption displayed a heterogeneous N-terminal end, with sequences starting at residues 5, 7, 9, and 11 of the predicted N-terminal portion of the mature protein. On the basis of structural and functional properties, cystatin D represents a novel cysteine proteinase inhibitor possibly playing a protective role against proteinases present in the oral cavity.
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| 4. |
- Freije, José P, et al.
(författare)
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Structure and expression of the gene encoding cystatin D, a novel human cysteine proteinase inhibitor
- 1991
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Ingår i: Journal of Biological Chemistry. - 1083-351X. ; 266:30, s. 20538-20543
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Tidskriftsartikel (refereegranskat)abstract
- A new member of the human cystatin multigene family has been cloned from a genomic library using a cystatin C cDNA probe. The complete nucleotide sequence of a 4.3-kilobase DNA segment, containing a complete gene with structure very similar to those of known Family 2 cystatin genes, was determined. The novel gene, called CST4, is composed of three exons and two introns. It contains the coding information for a protein of 142 amino acid residues, which has been tentatively called cystatin D. The deduced amino acid sequence includes a putative signal peptide and presents 51-55% identical residues with the sequences of either cystatin C or the secretory gland cystatins S, SN, or SA. The cystatin D sequence contains all regions of relevance for cysteine proteinase inhibitory activity and also the 4 cysteine residues that form disulfide bridges in the other members of cystatin Family 2. Northern blot analysis revealed that the cystatin D gene is expressed in parotid gland but not in seminal vesicle, prostate, epididymis, testis, ovary, placenta, thyroid, gastric corpus, small intestine, liver, or gall-bladder tissue. This tissue-restricted expression is in marked contrast with the wider distribution of all the other Family 2 cystatins, since cystatin C is expressed in all these tissues and the secretory gland cystatins are present in saliva, seminal plasma, and tears. Cystatin D, being the first described member of a third subfamily within the cystatin Family 2, thus appears to have a distinct function in the body in contrast to other cystatins.
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| 5. |
- Holm, Magnus, et al.
(författare)
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A Computer Algorithm for Determining Local Activation Times in Electrograms Obtained during Atrial Fibrillation
- 1993
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Ingår i: Proc. IEEE Computers in Cardiology, London, 5-8 Sep 1993. ; , s. 855-858
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Konferensbidrag (refereegranskat)abstract
- A critical factor in analyzing the propagation of activation waves in the myocardium is the definition of local activation times (LAT) in the electrogram. The authors have formulated and tested a definition of LAT in bipolar electrograms applicable to atrial electrograms containing deflections with wide variation in complexity, e.g. those obtained during rapid, irregular atrial rhythm, i.e. atrial fibrillation. The definition, implemented as an algorithm in a computer program, consists of two parts: the first part identifies local activations in the electrogram and the second calculates the corresponding LAT using criteria that define the LAT as the median activation time of cells located between the bipolar electrodes.
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| 6. |
- Holm, Magnus, et al.
(författare)
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Determination of Preferable Wavelet Propagation Directions in Atrial Fibrillation
- 1993
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Konferensbidrag (refereegranskat)abstract
- M. Holm, P. Blomström, J. Brandt, R. Johansson, C. Lührs, and S. B. Olsson. Determination of preferable wavelet propagation directions in atrial fibrillation. In Erik K. Fernström Symp. Atrial Fibrillation, Lund, Sweden, June 1993.
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| 7. |
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| 8. |
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| 9. |
- Jansson, Magnus, et al.
(författare)
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Efficient Implementation of a Submodel for Composite Materials to be Combined with the FDTD-Algorithm
- 1994
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Ingår i: IEEE Transactions on Magnetics. - : IEEE Magnetics Society. - 0018-9464 .- 1941-0069. ; 30:5, s. 3188-3191
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Tidskriftsartikel (refereegranskat)abstract
- A submodel to be used for thin sheets of semiconducting materials in combination with the finite difference time domain algorithm for solving Maxwell's equations is derived. Emphasis is concentrated on accomplishing an efficient and robust algorithm. Stability properties of the combined model are also investigated
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| 10. |
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