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Träfflista för sökning "(WFRF:(Mattsson H.)) srt2:(1995-1999) srt2:(1996)"

Search: (WFRF:(Mattsson H.)) srt2:(1995-1999) > (1996)

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1.
  • Andersson, K, et al. (author)
  • Gastric acid secretion after depletion of enterochromaffin-like cell histamine. A study with a-fluoromethylhistidine in rats
  • 1996
  • In: Scandinavian Journal of Gastroenterology. - 1502-7708. ; 31:1, s. 24-30
  • Journal article (peer-reviewed)abstract
    • BACKGROUND: Histamine is thought to play a central role in the regulation of gastric acid secretion. In the rat oxyntic mucosa most of the histamine is synthesized and stored in enterochromaffin-like (ECL) cells, and the rest resides in mast cells. The present study examines the role of ECL-cell histamine in the control of acid secretion in the intact, conscious rat. METHODS: Rats were treated with alpha-fluoromethylhistidine (alpha-FMH) to inhibit histamine synthesis. alpha-FMH was given by continuous subcutaneous infusion (3 mg/kg/h) for up to 9 days. An additional oral dose of alpha-FMH (50 mg/kg) was given 2 h before each acid secretion test. Acid secretion was studied in pylorus-ligated rats and in chronic gastric fistula rats stimulated with histamine, gastrin-17, or insulin after 2-6 days of alpha-FMH infusion. RESULTS: Treatment with alpha-FMH lowered oxyntic mucosal histamine synthesis by 80%. From previous observations this is thought to reflect depletion of histamine from the ECL cells. The remaining 20% resides in mucosal and submucosal mast cells, which seem to be resistant to alpha-FMH. Basal acid secretion was inhibited by more than 60% after alpha-FMH treatment and by more than 80% by ranitidine. Histamine-stimulated secretion was unaffected by alpha-FMH and abolished by the histamine H2-receptor antagonist ranitidine. The acid response to gastrin-17 was almost abolished in histamine-depleted rats and abolished by ranitidine. Vagally induced acid secretion (provoked by the injection of insulin or by pylorus ligation) was unaffected by alpha-FMH treatment but abolished by ranitidine and by the muscarinic M1-receptor antagonist pirenzepine. CONCLUSION: The results suggest that gastrin stimulates acid secretion by releasing histamine from ECL cells. Vagally induced acid secretion is also dependent on a histaminergic pathway but not on ECL-cell histamine.
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2.
  • Johansson, Jonas, et al. (author)
  • Remote fluorescence measurements of vegetation spectrally resolved and by multi-colour fluorescence imaging
  • 1996
  • In: Journal of Plant Physiology. - 0176-1617. ; 148:5, s. 632-637
  • Journal article (peer-reviewed)abstract
    • A remote sensing system for combined fluorescence spectral recordings and multi-colour fluorescence imaging of vegetation will be presented. The system configuration will be explained and data from several field campaigns will be shown. The excitation light pulses for remote fluorescence detection are produced by a frequency tripled Nd:YAG laser emitting at 355 nm and shifted to 397 nm in a deuterium containing Raman cell. The receiving optics includes a Newtonian telescope and is shared by an optical fibre bundle connected to an OMA system and by a secondary Cassegrainian split-mirror telescope for fluorescence imaging at four wavelength bands simultaneously. The multi colour fluorescence images are computer processed to form images of a ratio at two wavelengths or if necessary a more complex mathematical function including the four wavelength bands is used. Examples of remote multi-colour imaging of vegetation from the past field campaigns will be given. Remote fluorescence spectra from several plant species were recorded at an excitation wavelength of 397 nm. Furthermore, the system was found capable of producing high-quality single-shot spectra at a range of 125 m. Lastly, a recently developed push-broom scanning technique for fluorescence imaging will be discussed and some preliminary data will be shown.
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  • Result 1-3 of 3

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