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- Andersson, Fredrik, 1974, et al.
(författare)
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Computer Based Requirements and Concept Modelling - Information Gathering and Classification
- 2000
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Ingår i: 12th International Conference on Design Theory and Methodology. 2000 ASME International Design Engineering Technical Conferences.
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Konferensbidrag (refereegranskat)abstract
- This paper proposes a requirement and concept model based on a functional decomposition of mechanical systems. It is an object-oriented approach to integrate the representation of the design artefact and the design activity, through the decisions made during the design evolution. The requirements co-evolve simultaneously with the formation of the conceptual layout, through the opportunity to alter between function and physical/abstract solutions. This approach structures the design requirements and concepts in such a way that it supports the ability to document their sources, to allow for validation and verifications of both requirements and design solutions. First, the proposed model is presented from a theoretical viewpoint. Secondly, a methodology for modelling requirements and concepts in an object-oriented fashion is discussed. Finally, the model is implemented in METIS software and tested in a case study of an electric window winder on a truck door.
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| 2. |
- Ekström, Simon, et al.
(författare)
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Integrated microanalytical technology enabling rapid and automated protein identification
- 2000
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Ingår i: Analytical Chemistry. - : American Chemical Society (ACS). - 0003-2700 .- 1520-6882. ; 72:2, s. 286-293
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Tidskriftsartikel (refereegranskat)abstract
- Protein identification through peptide mass mapping by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has become a standard technique, used in many laboratories around the world. The traditional methodology often includes long incubations (6-24 h) and extensive manual steps. In an effort to address this, an integrated microanalytical platform has been developed for automated identification of proteins. The silicon micromachined analytical tools, i.e., the microchip immobilized enzyme reactor (μ-chip IMER), the piezoelectric microdispenser, and the high-density nanovial target plates, are the cornerstones in the system. The μ-chip IMER provides on-line enzymatic digestion of protein samples (1 μL) within 1-3 min, and the microdispenser enables subsequent on- line picoliter sample preparation in a high-density format. Interfaced to automated MALDI-TOF MS, these tools compose a highly efficient platform that can analyze 100 protein samples in 3.5 h. Kinetic studies on the microreactors are reported as well as the operation of this microanalytical platform for protein identification, wherein lysozyme, myoglobin, ribonuclease A, and cytochrome c have been identified with a high sequence coverage (50-100%).
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| 3. |
- Miliotis, Tasso, et al.
(författare)
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Protein identification platform utilizing micro dispensing technology interfaced to matrix-assisted laser desorption ionization time-of-flight mass spectrometry
- 2000
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Ingår i: Journal of Chromatography A. - 0021-9673. ; 886:1-2, s. 99-110
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Tidskriftsartikel (refereegranskat)abstract
- An integrated protein microcharacterization/identification platform has been developed. The system has been designed to allow a high flexibility in order to tackle challenging analytical problems. The platform comprises a cooled microautosampler, an integrated system for microcolumn HPLC, and a capillary reversed-phase column that is interfaced to matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) system via a low internal volume flow-through microdispenser. The chromatographic separation is continuously transferred onto a MALDI target plate as discrete spots as the dispenser ejects bursts of droplets of the column effluent in a precise array pattern. A refrigerated microfraction collector was coupled to the outlet of the flow-through microdispenser enabling enrichment and re-analysis of interesting fractions. The use of target plates pre-coated with matrix simplified and increased the robustness of the system. By including a separation step prior to the MALDI-TOF-MS analysis and hereby minimizing suppression effects allowed us to obtain higher sequence coverage of proteins compared to conventional MALDI sample preparation methodology. Additionally, synthetic peptides corresponding to autophosphorylated forms of the tryptic fragment 485-496 (ALGADDSYYTAR) of tyrosine kinase ZAP-70 were identified at sensitivities reaching 150 amol. Copyright (C) 2000 Elsevier Science B.V.
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