SwePub
Sök i SwePub databas

  Utökad sökning

Träfflista för sökning "(WFRF:(Oliveira e Costa Sandra)) srt2:(2020)"

Sökning: (WFRF:(Oliveira e Costa Sandra)) > (2020)

  • Resultat 1-2 av 2
Sortera/gruppera träfflistan
   
NumreringReferensOmslagsbildHitta
1.
  • Beal, Jacob, et al. (författare)
  • Robust estimation of bacterial cell count from optical density
  • 2020
  • Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
  • Tidskriftsartikel (refereegranskat)abstract
    • Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
  •  
2.
  • Stjernberg, Mats, et al. (författare)
  • Overcoming barriers to social inclusion in Nordic cities through policy and planning
  • 2020
  • Rapport (övrigt vetenskapligt/konstnärligt)abstract
    • This report examines how Nordic governments and municipalities seek to overcome barriers to social inclusion and to counteract inequality and segregation through policy and urban planning. Overcoming barriers to social inclusion is understood as the desire to improve the terms on which different individuals and groups take part in society through urban policy and planning while counteracting the negative effects of inequality. Examples of policy and planning initiatives to create more inclusive cities and communities can be found in all the Nordic countries. However, inclusion is a multifaceted issue and the specific challenges, and approaches to dealing with these challenges, vary among the countries and cities. To capture this diversity, this report examines five different thematic and geographical cases detailing strategies for inclusion from different perspectives in varying contextual settings.
  •  
Skapa referenser, mejla, bekava och länka
  • Resultat 1-2 av 2

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Stäng

Kopiera och spara länken för att återkomma till aktuell vy