| 1. |
|
|
| 2. |
|
|
| 3. |
|
|
| 4. |
|
|
| 5. |
|
|
| 6. |
|
|
| 7. |
- Lernmark, Å, et al.
(författare)
-
Heterogeneity of islet pathology in two infants with recent onset diabetes mellitus
- 1995
-
Ingår i: Virchows Archiv. - 0945-6317. ; 425:6, s. 631-640
-
Tidskriftsartikel (refereegranskat)abstract
- The mechanisms by which the beta cells of pancreatic islets are destroyed in insulin-dependent diabetes mellitus (IDDM) are poorly understood. In this report the pancreatic histo- and immunopathology of two children, both HLA-DR 3/4, DQ 2/8 positive and who both died from cerebral oedema within a day of clinical diagnosis of IDDM, were investigated. Patient 1, a 14-month-old girl, had a 4-week history of polydipsia and polyuria. Patient 2, a 3-year-old boy, had 2 days of illness. Both patients had a similarly severe loss of insulin cells but differed markedly as to the extent of lymphocytic islet infiltration (insulitis). Apart from insulitis, marked islet macrophage infiltration was demonstrated in both patients with the HAM-56 monoclonal antibody. Neither patient showed aberrant expression of HLA class II antigens on insulin-immunoreactive cells, but allele-specific HLA-DQ8 expression was evident on endothelial cells. Glutamic acid decarboxylase immunoreactivity was detected in both insulin- and glucagon-immunoreactive cells. It is concluded that the heterogeneity of islet pathology, especially insulitis, may reflect different dynamics and extent rather than different pathomechanisms of immune destruction of islets in IDDM.
|
|
| 8. |
- Petersen-Mahrt, S.K., et al.
(författare)
-
Effects of UV‐B radiation and nitrogen starvation on enzyme activities in isolated plasma membranes of Euglena gracilis
- 1995
-
Ingår i: Physiologia Plantarum. - : Wiley. - 0031-9317 .- 1399-3054. ; 95, s. 515-522
-
Tidskriftsartikel (refereegranskat)abstract
- Circadian rhythms are characteristic of many physiological and biochemical processes in the freshwater flagellate Euglena gracilis. Earlier, we found that the rhythms of photosynthesis, phototaxis and cell shape followed the same pattern in control organisms, but were differently affected by stress such as UV‐B irradiation and nitrogen deficiency. Here we extend our studies to use isolated plasma membranes to characterize the rhythms of some plasma membrane‐bound enzymes. Also, we wanted to see whether stress‐induced changes of these rhythms could be detected at the subcellular level and possibly be coupled to the changes seen in photosynthesis, phototaxis and cell shape. The isolation of plasma membranes using aqueous polymer two‐phase partitioning was successful, as judged by the large enrichment of the plasma membrane‐marker 5′‐nucleotidase, and the difference in the polypeptide pattern compared with the microsomal fraction from which it was prepared.
|
|
| 9. |
- Frostegård, A, et al.
(författare)
-
Dynamics of a microbial community associated with manure hot spots as revealed by phospholipid fatty acid analyses
- 1997
-
Ingår i: Applied and Environmental Microbiology. - 0099-2240. ; 63:6, s. 2224-2231
-
Tidskriftsartikel (refereegranskat)abstract
- Microbial community dynamics associated with manure hot spots were studied by using a model system consisting of a gel-stabilized mixture of soil and manure, placed between layers of soil, during a 3-week incubation period. The microbial biomass, measured as the total amount of phospholipid fatty acids (PLFA), had doubled within a 2-mm distance from the soil-manure interface after 3 days. Principal-component analyses demonstrated that this increase was accompanied by reproducible changes in the composition of PLFA, indicating changes in the microbial community structure. The effect of the manure was strongest in the 2-mm-thick soil layer closest to the interface, in which the PLFA composition was statistically significantly different (P < 0.05) from that of the unaffected soil layers throughout the incubation period. An effect was also observed in the soil layer 2 to 4 mm from the interface. The changes in microbial biomass and community structure were mainly attributed to the diffusion of dissolved organic carbon from the manure. During the initial period of microbial growth, PLFA, which were already more abundant in the manure than in the soil, increased in the manure core and in the 2-mm soil layer closest to the interface. After day 3, the PLFA composition of these layers gradually became more similar to that of the soil. The dynamics of individual PLFA suggested that both taxonomic and physiological changes occurred during growth. Examples of the latter were decreases in the ratios of 16:1 omega 7t to 16:1 omega 7c and of cyclopropyl fatty acids to their respective precursors, indicating a more active bacterial community. An inverse relationship between bacterial PLFA and the eucaryotic 20:4 PLFA (arachidonic acid) suggested that grazing was important.
|
|
| 10. |
- Karadima, G, et al.
(författare)
-
Nondisjunction studies in trisomy 8.
- 1997
-
Ingår i: CYTOGENETICS AND CELL GENETICS. - 0301-0171. ; 77:1-2, s. P105-P105
-
Konferensbidrag (övrigt vetenskapligt/konstnärligt)
|
|
