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- Mata, S, et al.
(författare)
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Multiple sclerosis is associated with enhanced B cell responses to the ganglioside GD1a
- 1999
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Ingår i: Multiple sclerosis (Houndmills, Basingstoke, England). - : SAGE Publications. - 1352-4585 .- 1477-0970. ; 5:6, s. 379-388
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Tidskriftsartikel (refereegranskat)abstract
- The occurrence and role of autoantibodies to gangliosides and other lipid-containing components of the central nervous system in Multiple Sclerosis (MS) are unsettled Using sensitive ELISAs, we measured IgG and JgM antibody titers and absorbances to the three major gangliosides GDIa, GD l b and GM, and to sulfatides, cardiolipin and myelin proteins in paired serum and cerebrospinal fluid (CSF) from patients with untreated MS, optic neuritis (ON), acute aseptic meningo-encephalitis (AM) and other neurological diseases (OND). Twenty-three per cent of 30 MS (P < 0.04) and 18% of 32 ON patient (P <0.05) presented elevated IgG antibody titers to GD) a in serum compared to 9% of patient with OND. Six (40%) of the patient with malignant MS had elevated serum IgG antibody titers to GD) a compared to one (6%) of th e patient with benign MS (P <0.04). In CSF, elevated IgG antibody titers to GDIa were measured in 13% of MS and 20% of ON patient compared to 4% of patient with OND (P < 0.03 and P < 0.02, respectively). The augmented IgG response to GD) a in serum also separated MS from Guillain-Barr6 syndrome. Compared to OND increased JgM absorbances to sulfatides and cardiolipin were observed in CSF of patient with MS, but also in AM. Elevated IgG antibody titers to myelin proteins were found more often in MS patient' serum and MS, ON and AM patient' CSF compared to OND. The data implicate that among the multitude of enhanced B-cell responses occurring in MS and ON, that directed to GD I a is common and more discriminative, and should be evaluated in future MS treatment studies.
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- Pinto do Ó, Perpétua, et al.
(författare)
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Expression of the LIM-homeobox gene LH2 generates immortalized Steel factor-dependent multipotent hematopoietic precursors
- 1998
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Ingår i: EMBO Journal. - : Oxford University Press. - 0261-4189 .- 1460-2075. ; 17:19, s. 5744-5756
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Tidskriftsartikel (refereegranskat)abstract
- The genes controlling self-renewal and differentiation in the hematopoietic system are largely unknown. The LIM-homeobox genes are known to be important for asymmetric cell divisions and differentiation of specific cell types and organs. One member of this family, LH2, is expressed in fetal liver at the time of active hematopoiesis. Therefore, we have assessed the function of LH2 during the formation and initial expansion of the hematopoietic system by differentiating LH2-transduced embryonic stem (ES) cells in vitro. This procedure generated multipotent hematopoietic precursor cell (HPC) lines that required Steel factor for growth. HPC lines have been maintained in an undifferentiated state in culture for >7 months. Other growth factors tested efficiently induce terminal differentiation of HPCs into various mature myeloid lineages. Steel factor is also required and acts synergistically with the other growth factors to generate multilineage colonies from the HPCs. These HPC lines express transcription factors that are consistent with an immature progenitor, and the pattern of cell surface marker expression is similar to that of early fetal multipotent hematopoietic progenitors. Collectively, these data suggest that the HPC lines represent an early fetal multipotent hematopoietic progenitor, and suggest a role for LH2 in the control of cell fate decision and/or proliferation in the hematopoietic system.
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