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> (2002) >
Delay between fusio...
Delay between fusion pore opening and peptide release from large dense-core vesicles in neuroendocrine cells.
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- Barg, Sebastian (author)
- Uppsala universitet,Lund University,Lunds universitet,Islet cell physiology,Forskargrupper vid Lunds universitet,Lund University Research Groups,Institutionen för medicinsk cellbiologi,Barg
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- Olofsson, Charlotta (author)
- Lund University,Lunds universitet,Medicinska fakulteten,Faculty of Medicine
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Schriever-Abeln, Jenny (author)
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- Wendt, Anna (author)
- Lund University,Lunds universitet,Diabetes - öcellsexocytos,Forskargrupper vid Lunds universitet,Diabetes - Islet Cell Exocytosis,Lund University Research Groups
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- Gebre-Medhin, Samuel (author)
- Lund University,Lunds universitet,Avdelningen för klinisk genetik,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Clinical Genetics,Department of Laboratory Medicine,Faculty of Medicine
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- Renström, Erik (author)
- Lund University,Lunds universitet,Institutionen för kliniska vetenskaper, Malmö,Medicinska fakulteten,Department of Clinical Sciences, Malmö,Faculty of Medicine
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- Rorsman, Patrik (author)
- Lund University,Lunds universitet,Islet cell physiology,Forskargrupper vid Lunds universitet,Lund University Research Groups
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(creator_code:org_t)
- 2002
- 2002
- English.
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In: Neuron. - 0896-6273 .- 1097-4199. ; 33:2, s. 287-299
- Related links:
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http://www.ncbi.nlm....
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http://dx.doi.org/10...
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Subject headings
Close
- Peptidergic neurotransmission is slow compared to that mediated by classical neurotransmitters. We have studied exocytotic membrane fusion and cargo release by simultaneous capacitance measurements and confocal imaging of single secretory vesicles in neuroendocrine cells. Depletion of the readily releasable pool (RRP) correlated with exocytosis of 10%-20% of the docked vesicles. Some remaining vesicles became releasable after recovery of RRP. Expansion of the fusion pore, seen as an increase in luminal pH, occurred after approximately 0.3 s, and peptide release was delayed by another 1-10 s. We conclude that (1) RRP refilling involves chemical modification of vesicles already in place, (2) the release of large neuropeptides via the fusion pore is negligible and only proceeds after complete fusion, and (3) sluggish peptidergic transmission reflects the time course of vesicle emptying.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
Keyword
- Peptides/*metabolism
- PC12 Cells
- Neurosecretory Systems/cytology/*metabolism
- Membrane Fusion/*physiology
- Luminescent Proteins
- Kinetics
- Indicators and Reagents
- Hydrogen-Ion Concentration
- Exocytosis/*physiology
- Fluorescence
- Rats
- Secretory Vesicles/*metabolism
- Support
- Non-U.S. Gov't
- Electric Stimulation
- Cell Line
- Animal
Publication and Content Type
- art (subject category)
- ref (subject category)
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