| 1. |
- Chattopadhyay, S., Pal, S., Pal, D., Sarkar, D., Chandra (Sanyal), S., DasGupta, C.
(författare)
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Protein folding in Escherichia coli: role of 23S ribosomal RNA
- 1999
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Ingår i: Biochim Biophys Acta. ; 1429:2, s. 293-8
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Tidskriftsartikel (refereegranskat)abstract
- Post-translational control of Escherichia coli ribosome on newly synthesised polypeptide leading to its active conformation (protein folding) has been shown in the case of the enzyme beta-galactosidase. As expected, antibiotics chloramphenicol and lincomy
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| 2. |
- Pal, S., Chandra (Sanyal), S., Chowdhury, S., Sarkar, D., Ghosh, A.N., DasGupta, C.
(författare)
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Complementary role of two fragments of domain V of 23 S ribosomal RNA in protein folding
- 1999
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Ingår i: J Biol Chem. ; 274:46, s. 32771-7
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Tidskriftsartikel (refereegranskat)abstract
- We have shown that the domain V of bacterial 23 S rRNA could fold denatured proteins to their active state. This segment of 23 S rRNA could further be split into two parts. One part containing mainly the central loop of domain V could bind denatured human
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| 3. |
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| 4. |
- Pal, D., Chattopadhyay, S., Chandra (Sanyal), S., Sarkar, D., Chakraborty, A., DasGupta, C.
(författare)
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Reactivation of denatured proteins by domain V of bacterial 23S rRNA
- 1997
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Ingår i: Nucleic Acids Res. ; 25:24, s. 5047-51
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Tidskriftsartikel (refereegranskat)abstract
- In vitro transcripts containing domain V of the 23S rRNA of Escherichia coli and Bacillus subtilis can reactivate denatured proteins almost as efficiently as the total 23S rRNA. Here we show that almost the full length of domain V is required for reactiva
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| 5. |
- Adams, JA, et al.
(författare)
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Cosmic microwave background anisotropy in the decaying neutrino cosmology
- 1998
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Ingår i: MONTHLY NOTICES OF THE ROYAL ASTRONOMICAL SOCIETY. - : BLACKWELL SCIENCE LTD. - 0035-8711. ; 301:1, s. 210-214
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- It is attractive to suppose for several astrophysical reasons that the Universe has close to the critical density in light (similar to 30 eV) neutrinos which decay radiatively with a lifetime of similar to 10(23) s. In such a cosmology the Universe is re-
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| 6. |
- Adams, JA, et al.
(författare)
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Multiple inflation
- 1997
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Ingår i: NUCLEAR PHYSICS B. - : ELSEVIER SCIENCE BV. - 0550-3213. ; 503:1-2, s. 405-425
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- Attempts at building a unified description of the strong, weak and electromagnetic interactions usually involve several stages of spontaneous symmetry breaking. We consider the effects of such symmetry breaking during an era of primordial inflation in sup
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| 7. |
- Adams, JA, et al.
(författare)
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Natural supergravity inflation
- 1997
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Ingår i: PHYSICS LETTERS B. - : ELSEVIER SCIENCE BV. - 0370-2693. ; 391:3-4, s. 271-280
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- We identify a new mechanism in supergravity theories which leads to successful inflation without any need for fine-tuning. The simplest model yields a spectrum of density fluctuations tilted away from scale-invariance and negligible gravitational waves. W
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| 8. |
- Coenen Schimke, J. M., et al.
(författare)
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A quantitative PCR measurement of messenger RNA expression of IGF-I, IGF-II and IGFBP-5 in human skeletal muscle
- 1999
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Ingår i: Growth Hormone & IGF Research. - : Elsevier. - 1096-6374 .- 1532-2238. ; 9:3, s. 179-186
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Tidskriftsartikel (refereegranskat)abstract
- Insulin-like growth factor-I and -II (IGF-I and IGF-II) and their binding proteins are important components in growth promotion and tissue maintenance. We determined the presence of IGF-I, -II, and binding protein 5 (IGFBP-5) gene expression in human skeletal muscle and that mRNA abundance is not altered by nutrients and insulin. In the first protocol, (control) subjects were given water. In the second protocol, half of these subjects drank Polycose (carbohydrate) and the remaining subjects drank equal calories as a mixed meal. Quadriceps muscle biopsies were taken at 10 h. A semi-quantitative polymerase chain reaction was designed to measure gene expression. IGF-I, IGF-II and IGFBP-5 mRNA are present in adult human skeletal muscle, but no significant changes between meal groups were observed for IGF-I, IGF-II or IGFBP-5 mRNA levels, indicating that the expression of these genes are not altered acutely by nutrients and insulin.
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| 9. |
- Sarkar, S, et al.
(författare)
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In the beginning (vol 158, pg 7, 1998)
- 1998
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Ingår i: NEW SCIENTIST. - : NEW SCIENTIST PUBL EXPEDITING INC. ; 158:2133
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Annan publikation (övrigt vetenskapligt/konstnärligt)
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| 10. |
- Tomac, S., et al.
(författare)
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Ionic effects on the stability and conformation of peptide nucleic acid complexes
- 1996
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Ingår i: Journal of the American Chemical Society. - : American Chemical Society (ACS). - 1520-5126 .- 0002-7863. ; 118:24, s. 5544-5552
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Tidskriftsartikel (refereegranskat)abstract
- Peptide nucleic acid (PNA) is a DNA analogue in which the negatively charged sugar phosphate backbone has been substituted by uncharged N-(2-aminoethyl)glycine units. The study of a PNA-DNA duplex and the corresponding DNA-DNA duplex gives a unique opportunity to compare two polyelectrolytes with virtually identical geometry but greatly different linear charge density. The results provide a basis for a study of the applicability of the Poisson-Boltzmann (PB) and counterion condensation (CC) theories. UV and circular dichroism spectroscopy as well as isothermal titration calorimetry (ITC) have been used to study the effect of different ions on the stability and conformation of PNA-DNA, PNA-PNA, and DNA-DNA duplexes having the same base sequences. Cations in general destabilize both antiparallel (N/3') and parallel (N/5') PNA-DNA duplexes whereas they stabilize the DNA-DNA duplex. Studies on the effect of monovalent salt such as NaCl on T-m were carried out over a wide range of salt concentrations (0.01 to 5 M). The decrease in the T-m of the N/3' PNA-DNA duplex with increasing ionic strength in the range of concentrations of 0.01 to 0.5 M, where electrostatic effects predominate, is explained in terms of counterion release upon duplex formation in contrast to the counterion association accompanying the formation of a DNA duplex. The uncharged PNA-PNA duplex shows no significant destabilization in this concentration range. The higher stability of the N/3' PNA-DNA compared to the DNA-DNA duplex (Delta Delta G similar to-7 kcal/mol) is ascribed to more favorable entropic contributions consistent with the counterion release that accompanies the PNA-DNA duplex formation. At high salt concentration (>1 M), where electrostatic contributions saturate, similar trends in the decrease in T-m, were observed for the three types of duplexes irrespective of their backbone charges. The destabilizing effects of a series of Na salts with various monovalent anions on N/3' PNA-DNA and PNA-PNA duplexes were found to follow the Hofmeister series, emphasizing the importance of the hydrophobic interaction between nucleobases for the stability of the PNA complexes in high salt concentration.
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