| 1. |
- Cao, RH, et al.
(författare)
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Suppression of angiogenesis and tumor growth by the inhibitor K1-5 generated by plasmin-mediated proteolysis
- 1999
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Ingår i: Proceedings of the National Academy of Sciences of the United States of America. - : Proceedings of the National Academy of Sciences. - 0027-8424. ; 96:10, s. 5728-5733
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Tidskriftsartikel (refereegranskat)abstract
- Proteolytic enzymes are involved in generation of a number of endogenous angiogenesis inhibitors. Previously, we reported that angiostatin, a potent angiogenesis inhibitor, is a proteolytic fragment containing the first four kringle modules of plasminogen. In this report, we demonstrate that urokinase-activated plasmin can process plasminogen to release an angiogenesis inhibitor, K1–5 (protease-activated kringles 1–5). K1–5 inhibits endothelial-cell proliferation with a half-maximal concentration of approximately 50 pM. This inhibitory effect is endothelial-cell-specific and appears to be at least approximately 50-fold greater than that of angiostatin. A synergistic efficacy of endothelial inhibition was observed when angiostatin and kringle 5 (K5) were coincubated with capillary endothelial cells. The synergistic effect is comparable to that produced by K1–5 alone. Systemic treatment of mice with K1–5 at a low dose significantly blocked the fibroblast growth factor-induced corneal neovascularization, whereas angiostatin had no effect at the same dose. K1–5 also suppressed angiogenesis in chicken embryos. Systemic administration of K1–5 at a low dose at which angiostatin was ineffective significantly suppressed the growth of a murine T241 fibrosarcoma in mice. The antitumor effect correlates with the reduced neovascularization. These findings suggest that the plasmin-mediated proteolysis may be involved in the negative switch of angiogenesis.
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| 2. |
- Christensen, P. W., et al.
(författare)
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Formulation and comparison of algorithms for frictional contact problems
- 1998
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Ingår i: International Journal for Numerical Methods in Engineering. - : John Wiley & Sons. - 0029-5981 .- 1097-0207. ; 42:1, s. 145-173
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Tidskriftsartikel (refereegranskat)abstract
- This paper presents two algorithms for solving the discrete, quasi-static, small-displacement, linear elastic, contact problem with Coulomb friction. The algorithms are adoptions of a Newton method for solving B-differentiable equations and an interior point method for solving smooth, constrained equations. For the application of the former method, the contact problem is formulated as a system of B-differentiable equations involving the projection operator onto sets with simple structure; for the application of the latter method, the contact problem is formulated as a system of smooth equations involving complementarity conditions and with the non-negativity of variables treated as constraints. The two algorithms are numerically tested for two-dimensional problems containing up to 100 contact nodes and up to 100 time increments. Results show that at the present stage of development, the Newton method is superior both in robustness and speed. Additional comparison is made with a commercial finite element code.
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| 3. |
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| 5. |
- Shi, L X, et al.
(författare)
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Characterisation of the PsbX protein from Photosystem II and light regulation of its gene expression in higher plants
- 1999
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Ingår i: Plant Molecular Biology. - 0167-4412 .- 1573-5028. ; 40:4, s. 737-744
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Tidskriftsartikel (refereegranskat)abstract
- The location and expression of the previously uncharacterised photosystem II subunit PsbX have been analysed in higher plants. We show that this protein is a component of photosystem II (PSII) core particles but absent from light-harvesting complexes or PSII reaction centres. PsbX is, however, localised to the near vicinity of the reaction centre because it can be cross-linked to cytochrome b559, which is known to be associated with the D1/D2 dimer. We also show that the expression of this protein is tightly regulated by light, since neither protein nor mRNA is found in dark-grown plants.
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